New antigen display system based on insect viral capsid and construction method of new antigen display system

Abstract

The invention discloses a new antigen display system based on insect viral capsid and a construction method of the new antigen display system. The steps of the system construction method are as follows: (1) determining an insertion site of a heterologous protein; (2) successfully constructing a library of WhNV recombinant viroid particle VLP fused with enhanced green fluorescent protein EGFP; (3)constructing a library of Wuhan Noda Village recombinant virus particles fused with H5 antigen protein of human avian influenza virus; and (4) establishing a virus display system of Wuhan Noda Village. The system comprises: (1) WhNV capsid of Wuhan Noda Village virus, (2) a fusion protein as the heterologous protein inserted into the WhNV capsid protein, and (3) linkers, small amino acid sequencesrespectively added to the N-terminal and C-terminal of the fusion protein as linkers to connect with the WhNV capsid. According to the present invention, the construction method is simple and easy tooperate, and the WhNV heterologous antigen display system successfully established by the method lays a good foundation for the establishment of an efficient, stable and highly versatile platform forthe development of influenza VLP vaccine, and then for the establishment of platforms for the development of multiple vaccines and drugs.

Description

technical field [0001] The invention belongs to the technical field of vaccine preparation, and in particular relates to a method for constructing a display system for efficiently displaying human avian influenza virus antigens on the surface of WhNV capsid VLPs, and also relates to a method for efficiently displaying human avian influenza virus on the surface of WhNV capsid VLPs Antigen display system, which will lay the foundation for the research and development of avian influenza vaccines, and the establishment of a stable and efficient WhNV heterologous polypeptide and protein display system will greatly promote the research and development of other new vaccines and antidotes, which has considerable application prospects. Greatly promote the development of biomedicine. Background technique [0002] The surface display technology of using viral capsid VLPs to display heterologous polypeptides and protein antigens on its surface has become the current international biolog...

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Problems solved by technology

In this regard, human papillomavirus type 16 (HPV16) VLPs, Johnson Grass Mosaic virus (JGMV) VLPs, HBV core protein VLPs, etc., have been used for surface display of polypeptide antigens and vaccine development [Sadeyen JR et al. 2003; Saini M et al. 2003; Storni T et al. 2004], in 2008, Canadian scientists used the VLPs of papaya mosaic virus (PapMV) to successfully display the 2-24 amino acid peptide (M2e ), and proved that it has a good immune protection against the lethal challenge of virulent strains of mice, and may become a new method for the development of broad-spectrum influenza virus vaccines [Denis J et al. 2008]. There are limitations. In most cases, only a few to dozens of amino acid peptides of heterologous antigens can be displayed, thus affecting its complete immunogenicity and lacking in producing lasting immune effects [López- Macías C et al. 2012]

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