Method for identifying hybrid germplasm of actinidia based on genome heterozygosity

A kiwifruit and heterozygosity technology, applied in the field of plant genetic resource evaluation and biology, can solve problems such as bias, incomplete marker coverage, errors, etc., and achieve the effect of saving time and reducing unit sequencing cost.

Inactive Publication Date: 2015-05-20
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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Problems solved by technology

However, due to the limitations of the markers used in traditional heterozygosity assessment, the comparison of interspecies results is unreliable, especially when hybrid introgression only produces genetic exchange in a part of the genome region of the species, and the incomplete coverage of markers can lead to bias and even errors. the result of

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  • Method for identifying hybrid germplasm of actinidia based on genome heterozygosity
  • Method for identifying hybrid germplasm of actinidia based on genome heterozygosity
  • Method for identifying hybrid germplasm of actinidia based on genome heterozygosity

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Embodiment Construction

[0026] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0027] 1. Method

[0028] 1. Step ①

[0029] Such as figure 1 , Genome low-depth sequencing-1

[0030] Selection of ancestral basic species of kiwifruit and sequencing strategies suitable for hybrid germplasm identification.

[0031] A. Screening 10 basic ancestral species of kiwifruit

[0032] According to the existing Actinidia species group, these basic species evolved independently, representing more than 90% of the morphological and geographical distribution variation of the plants of the genus, while the genome was basically not eroded by exogenous genetic material;

[0033] B. Perform 5-fold genome low-depth sequencing on these 10 ancestral basic species and germplasm to be identified

[0034] According to the genome size of kiwifruit about 700M, the amount of data obtained is about 3.5G, which can meet the identification requirements of this embodiment.

[0035] The specif...

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Abstract

The invention discloses a method for identifying hybrid germplasm of actinidia based on genome heterozygosity, relating to the technical fields of plant genetic resources and biology. The method comprises the following steps: (1) performing low-depth genome sequencing on a sample; (2) comparing reference genomes of sequencing data and mining single-base mutation sites; (3) calculating the heterozygosity of genome level of the sample based on the single-base mutation sites; (4) constructing a basic species heterozygosity distribution rule database of a main ancestor of the actinidia; and (5) performing heterozygosity comparison on a to-be-detected sample and the database so as to identify the hybrid germplasm. Compared with the traditional molecular marker technology, the method disclosed by the invention has the advantages that the identification accuracy is greatly improved; and meanwhile, compared with the traditional morphological identification method, the method disclosed by the invention is high-efficiency and rapid, and the identification time and cost can be saved. The method disclosed by the invention has wide applicability on different sequencing platforms, and comprehensive evaluation and application of the germplasm resources of actinidia can be formed.

Description

technical field [0001] The invention relates to the fields of plant genetic resource evaluation and biotechnology, in particular to a method for identifying kiwifruit hybrid germplasm based on genome heterozygosity. It specifically involves low-depth genome sequencing of Actinidia plant germplasm and genome heterozygosity calculation based on sequencing data, basic database construction of genome heterozygosity of main ancestral species groups, comparative analysis of germplasm to be identified and final hybridization of germplasm identification identification. Background technique [0002] Actinidia ( Actinidia Lindl.) Plants have 54 species and about 75 different taxa. Among the various genetic germplasm resources of kiwifruit collected and preserved in nature and artificially, there are a large number of hybrid germplasm resources due to its botanical characteristics of dioecious perennial. Due to the integration of different genetic resources and genomes, hybridizati...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6869C12Q2537/165
Inventor 刘义飞李大卫黄宏文
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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