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Method for creating cymbidium resource for high-efficiency generation of 2n male gametes

A male gamete, high-efficiency technology, applied in the field of plant genetics and breeding, can solve the problems of 2n male gamete competitiveness decline, low induction rate, unstable efficiency, etc., and achieve the effect of improving core competitiveness, promoting development, and high vitality

Active Publication Date: 2014-04-09
英德君泓兰花股份有限公司
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AI Technical Summary

Benefits of technology

In this new way of breeding plants called symbiales, there has been developed techniques like crossing them together from different directions - which makes it easier than traditional ways such as creating seeds by hand-planters. These flowers will also grow well even if they were damaged during their growth process due to environmental factors like drought conditions.

Problems solved by technology

This patents discusses different techniques for producing high quality orchid seed from plants called Actigradea sppachioides L.) due to its importance in industry culture. However, current approaches involve manual laborious processes like crossing generations between parental lines, resulting in lower yields compared to natural ones. Additionally, existing plant selection strategies aim towards generating specific types of fertilizers based solely upon their ability to reproduce certain traits found within this genus.

Method used

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  • Method for creating cymbidium resource for high-efficiency generation of 2n male gametes
  • Method for creating cymbidium resource for high-efficiency generation of 2n male gametes
  • Method for creating cymbidium resource for high-efficiency generation of 2n male gametes

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Embodiment 1

[0037] Example 1 The creation of high-efficiency 2n male gamete resources of Jinzui × rabbit ear orchid hybrids

[0038] S1. Distant hybrid combination configuration: during the flowering stage of the golden mouth orchid, choose a healthy and disease-free plant as the female parent, take the pollen block of the rabbit ear orchid to pollinate it, mark the hybrid combination name and hybridization time on the plastic card with a pencil, and hang it on the plastic card. on the mother plant. Cultivate the female plant under suitable conditions to obtain fruit.

[0039] S2. Hybrid population construction

[0040] S21. Disinfection of fruit pods and germination of seeds: Remove the stalks of the freshly harvested fruits with a scalpel, dip in an appropriate amount of detergent to clean them, and rinse them with tap water for 2 to 3 minutes. % alcohol soaked for 30s, transferred to 0.1% mercuric chloride for disinfection for 15min, rinsed with sterile water for 5-8 times ...

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Abstract

The invention relates to the field of plant genetic breeding technology, and particularly discloses a method for creating a cymbidium resource for high-efficiency generation of 2n male gametes. The method includes parent matching, distant hybridization combination, hybrid colony construction, 2n male gamete identification, screening of the resource for high-efficiency generation of the 2n male gametes and the like. With utilization of the distant hybridization method, the cymbidium resource for high-efficiency generation of the 2n male gametes is obtained, and lays the foundation for high-efficiency creation of sexual polyploids by utilizing a sexual hybridization method, effective promotion of cymbidium polyploidy breeding, and cultivation of a breakthrough new cymbidium species in the absence of polyploids.

Description

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Claims

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Application Information

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Owner 英德君泓兰花股份有限公司
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