The invention discloses highland barley flavored beef jerky with barley fragrance and a preparation method of the highland barley flavored beef jerky. The highland barley flavored beef jerky is prepared from the following raw materials in parts by weight: 16-18 parts of highland barley, 240-250 parts of beef, 18-20 parts of barley 
seedling leaves, 6-8 parts of common turnips, 8-10 parts of 
semen cassiae, 2-3 parts of villous 
amomum fruits, 1-2 parts of radix angelicae, 1-2 parts of fennel fruits, 1-2 parts of nardostachyos root and 
rhizome, 6-8 parts of table salt, 4-6 parts of mango cores, 2-3 parts of Chinese peashrub roots, 2-4 parts of pomegranate seeds, 2-4 parts of pineapple peel, 4-5 parts of yellow ginger 
powder, 6-8 parts of tender 
shoot of 
Koelreuteria paniculata, an appropriate amount of 
pomegranate seed oil and 20-30 parts of haw 
wine. The highland barley flavored beef jerky with barley fragrance, prepared by the preparation method disclosed by the invention, is fragrant, crisp and chewy, integrates the fragrance and the 
flavor of the highland barley and the fragrance and the 
flavor of barley, can clear the intestines, ease 
constipation, lose weight, strengthen bodies, and enhance the 
immunity of organisms, and is unique in 
flavor; according to the formula of the highland barley flavored beef jerky with barley fragrance, varied 
traditional Chinese medicine ingredients are also added, so that health-care functions are improved, and the highland barley flavored beef jerky with barley fragrance has the efficacies of invigorating the 
stomach, promoting 
digestion, clearing the 
lung, promoting 
blood circulation, resisting oxidation, clearing away heart fire, reducing pathogenic fire and the like; the beef wrapped with barley straws is roasted, so that the barley fragrance is generated, the highland barley flavored beef jerky is rich in flavor, and the highland barley flavored beef jerky with barley fragrance is a novel flavored jerky product with the advantages of being environmentally-friendly, health-care, comprehensive in 
nutrition, leisure, convenient and the like.