97 results about "Cis-regulatory element" patented technology

Disclosed are nucleic acid vectors which comprise: (a) a transfer nucleotide sequence comprising (i) a plant active promoter, operably linked to (ii) a recombinant tobacco rattle virus (TRV) cDNA (preferably derived from TRV RNA2) which includes at least cis acting elements permitting replication of the cDNA; a subgenomic promoter operably linked to a sequence encoding a TRV coat protein; and a heterologous nucleotide sequence which is foreign to the virus;(b) border sequences which permit the transfer of the transfer nucleotide sequence into a plant genome. Such vectors may be used as expression vectors or for achieving viral induced gene silencing (VIGS) of a target gene, wherein the heterologous nucleotide sequence is a targeting sequence which corresponding to that gene. Example vectors include pTV00 and vectors which are derived from PTV00 and have the characteristics thereof. Also disclosed are associated processes, methods, viruses or viral particle, kits, host cells and plant tissues.

Isolated polynucleotide sequences exhibiting endothelial cell specific promoter activity, novel cis regulatory elements and methods of use thereof enabling treatment of diseases characterized by aberrant neovascularization or cell growth are disclosed.

Compositions, methods and kits are provided that are useful, for example, for determining activities of multiple cis-regulatory sequences, such as promoters and enhancers, and/or multiple trans-acting factors, such as transcription factors, in a cell. In particular, in certain embodiments, compositions are provided comprising a population of polynucleotide reporter transcription units (RTUs) in which each RTU comprises a reporter sequence, a processing tag located in the reporter sequence; and a cis-regulatory element operably linked to the reporter sequence, wherein the reporter sequences between any two RTUs in the population, outside of the processing tags, are substantially identical and wherein the positions of the processing tags within the reporter sequences distinguish between any two RTUs differing, for example, in their cis-regulatory elements. The compositions, methods and kits can further be used, for example, to identify a cell type or disease state, for example, in a biological organism.

A method for using the promotor LEAP of rice's drought inducting gene to improve the resistance of plant to drought features that the promotor LEAP of rice OsLEAl gene is cloned. Its nucleotide sequence is shown by SEQ ID No.1 and is used to code a drought induced expression protein. Two cis-acting elements ABRE contained in its promotor region can be specifically response to drought and ABA. Its inductive expression carrier can stongly induce the expression of target gene when the drought stress is applied to a plant. The method for culturing the drought-resistant plant is also disclosed.

The invention discloses a promotor CPIP from Oryza Sativa L in the plant genetic engineering domain, which is characterized by the following: the promotor CPIP possesses SEQ ID NO: 1 with nucleic acid sequence, which controls two alfapsin inhibition sub-genes simultaneously; the alfapsin inhibition sub-gene is controlled by promotor CPIP in backward-forward direction, which makes answering reaction with arid, salt-forced and abscisic acid(ABA); the promotor region contains a plurality of hostile environment answering cis-form appliance elements; the promotor CPIP controls GUS report gene in backward-forward direction to converse rice; the GUS gene is induced and expressed strongly in the condition of arid and salt-forced.

The invention relates to an induction-enhanced tissue specific promoter and application thereof, belonging to the technical field of plant genetic engineering. A promoter (BPHP, i.e. Bph14 promoter) for Oryza Sativa L. genes resistant to brown plant hoppers is separated from Oryza Sativa L. and is cloned. The promoter has a nucleotide sequence shown as SEQ ID No. 1, can control Oryza Sativa L. genes resistant to the brown plant hoppers to be specifically expressed in vascular bundle tissues and can be expressed in a way that the feeding induction of the brown plant hoppers is enhanced. The promoter contains BS1EGCCR cis-acting elements and can enable the genes to be specifically expressed in plant vascular bundle tissues. Moreover, the promoter has defense-related response cis-acting elements which can be combined with WRKY transcription factors, and the gene expression is increased when Oryza Sativa L. is fed by the brown plant hoppers or is infected by pathogenic bacteria. The promoter can be used as the promoter for the specific expression in the vascular bundle tissues and can be used as the brown plant hopper and pathogenic bacteria induction-enhanced promoter.

The invention discloses the corn resisting transcription and adjusting factor and coding gene and appliance. The corn resisting transcription adjusting factor is among the proteid of the following amino acid residue: 1) the SEQ ID No:1 of the sequence list; 2)the amino acid residue sequence of the SEQ ID No:1 of the sequence list are replaced, appended by one to ten amino acid residue and the proteid acting with the LTRE arranging component to improve the capability of resisting contradictorily of the plane. The adjusting factor can be acted on the LTRE arranging component of the several resisting abiotic adversity dependency gene promoter zone and adjust several the expression of the resisting abiotic adversity dependency gene and improve the ability of the plane resisting the abiotic adversity such as the drought, the low temperature and the salting. So the LBF is important to breed the resisting athwart plant species particularly the corn and improve the yield of the crop particularly the corn.

The invention discloses an ERF transcription factor related to the plant stress resistance and an encoding gene and application thereof. The invention firstly provides an ERF transcription factor gene LcERF056 separated from lotus japonicas, the amino acid sequence of the gene LcERF056 is shown as SEQ ID No.1, and the nucleotide sequence of the encoding transcription factor is shown as SEQ ID No.2. The invention further provides an ERF transcription factor structure domain which can be combined with a cis-acting element to start stress resistance response gene expression and is shown as SEQ ID No.3. Function analysis experiments prove that the resistance of a plant to high salt stress can be effectively enhanced or improved by overexpressing the LcERF056 gene in the plant, and therefore the protein encoded by the LcERF056 gene has the functions of the EFR transcription factor. The invention further provides application of the ERF transcription factor and the encoding gene on the aspects of enhancing the adversity stress resistance of the plant, breeding new transgenic plant varieties with the adversity stress resistance and the like.

The invention discloses a corn callus specific promoter and a cloning method and application thereof. A WRKY gene is obtained by utilizing bioinformatics analysis, the sequence comparison of the gene and the genome is performed, a primer is designed to perform polymerase chain reaction (PCR) and obtain the promoter region of the gene 5', the promoter is 2880bp and has the sequence shown in the (400)1 item of the sequence table; the analysis of the promoter shows that the promoter contains a plurality of acting elements; and the promoter is subcloned to the vector with GUS gene, expression of the GUS gene is started, and the vector is transformed in rice. The staining examine of each tissue of rice shows that the specificity of the promoter can be expressed in callus, thus the promoter hasimportant application value in genetic engineering and the gene function researches.

The invention discloses a promoter. The promoter can regulate and control the specific expression of a gene in secretion-type glandular hair, and a nucleotide sequence of the AaALDH1 gene promoter is shown as SEQ ID NO.1. The invention also provides an application of the promoter in the genetic engineering breeding by adopting plant secretion-type glandular hair tissues to express and produce metabolite. In addition, the invention also discloses a carrier and an expression cassette containing the promoter. By adopting the AaALDH1 gene promoter, a foundation is set for researching the expression regulation and control of the AaALDH1 gene and analyzing a cis acting element on the promoter, and the important significance on the genetic engineering breeding utilizing the plant secretion-type glandular hair tissues to express and produce the metabolite also can be realized.

A gene capable of increasing an expression level of aspergillus niger xylanase, a recombinant plasmid and a host cell thereof relate to optimal codon optimization, GC content adjusting and optimization of cis-acting element and sequential-repetitive sequence on a xylanase gene from aspergillus niger. According to design of a site-directed mutagenesis primer, artificial reconstruction on an original aspergillus niger xylanase gene is carried out to obtain an xylanase gene xynBT over-expressed in pichia pastoris. The reconstructed aspergillus niger xylanase gene xynBT has an expression level in pichia pastoris increased by 2.6 times, compared with that of an original gene xynB; during high density fermentation in a 3 L fermentation cylinder, the xylanase gene xynBT has an expression level in pichia pastoris reaching 20424.2 U / mL. The invention can be applied to molecule reconstruction on the xylanase gene and production of a recombinant xylanase, and can substantially increase the expression level of the xylanase.

The invention discloses a system for rapidly analyzing an RNA (ribonucleic acid) functional element in vivo. The system comprises a bifluorescence reporter vector p35mG, and the vector comprises a coding sequence shown as SEQ ID No.1. Through establishment of the bifluorescence reporter vector p35mG with the coding sequence shown as the SEQ ID No.1, one fluorescent protein mCherry is taken as internal reference, the other fluorescent protein GFP is taken as a reporter gene, a to-be-detected gene segment is inserted into GFP coding sequence to form a recombinant reporter gene GFP-3'UTR, and the functional cis acting element in the RNA is rapidly identified by analyzing influence of different segments on fluorescence intensity ratio of GFP to mCherry; besides, the regulation process of the cis acting element can be controlled through application of different treatment or expression of different effector proteins, and accordingly, the regulation effect of different signals or effector proteins in plants on the RNA is studied.

The invention belongs to the technical field of plant genetic engineering and specifically relates to a method for screening a rice tissue specific expression type cis-acting element and a flanking sequence thereof. According to the information in a rice whole-growth-period expression profile chip database, all tissue specific expression genes are classified according to expression patterns. According to the information in a plant cis-acting element database, cis-acting elements in the promoter regions of different rice tissue specific expression genes are scanned and counted. After that, cis-acting elements in the promoter regions of green tissue specific expression genes, which occur at a high frequency, are selected as candidate elements related to green tissue specific expression. Meanwhile, the flanking sequence of one candidate element GEAT is subjected to bioinformatic prediction. The combination of the above element and the flanking sequence thereof is integrated with -46 Minimal 35S promoter, so that the gene expression of a GUS gene is driven for function identification. The GUS analysis shows that, the GUS gene is associated with the green tissue specific expression. Through the site-directed mutagenesis experiment of the flanking sequence, a basic group having a critical role in maintaining the function of GEAT is found.

The invention discloses a lotus corniculatus AP2/ERF transcription factor and an encoding gene and application thereof and belongs to the field of AP2/ERF transcription factors and application. The invention discloses the gene of an AP2/ERF transcription factor separated from lotus corniculatus. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The amino acid sequence of protein encoded by the gene of the transcription factor is shown as SEQ ID NO.2. The invention further discloses an AP2/ERF transcription factor structural domain which can be combined with a cis-acting element to start adverse-resistant response gene expression, and the amino acid sequence of the structural domain is shown as SEQ ID NO.3. Function conversion experiments prove that the gene of the AP2/ERF transcription factor in a plant can improve salt tolerance of the transgenic plant remarkably, and it shows that the protein encoded by the gene of the transcription factor has a function of the AP2/ERF transcription factor. The gene of the AP2/ERF transcription factor has an important application prospect in improving resistance of the plant to adversity stress.

The invention discloses a cotton fiber specificity promoter GhFLA1 and a gene complete sequence thereof, relating to an important regulatory element related to cotton fiber development. The FLA1 gene complete sequence has totally 1920bp and comprises a 5' end promoter and a gene coding sequence. The gene does not contain an intron and encodes an arabinogalactan protein (AGP). The cotton fiber contains a great amount of the AGP protein, and that AGP irreversible inhibitor can inhibit fiber elongation shows that the protein has an important role for cotton fiber development. That the FLA1 gene mRNA has a great amount of specificity accumulation in the process of cotton fiber cell development shows that the gene promoter plays an important role in the regulation of fiber specificity expression. Experiments also show that the promoter has strong expression activity in tobacco leaf epidermal hair, which further proves that the promoter is the specificity regulatory element of the cotton fiber. The promoter can be used as a gene cis-regulatory element to be applied to cotton molecular breeding research so as to improve the cotton fiber quality and to enhance the cotton yield.

The invention discloses a soybean low-temperature inducing promoter, a recombinant expression vector containing the same, an application of the soybean low-temperature inducing promoter and particularly relates to the field of plant genetic engineering, in particular to expression of a soybean gene promoter sequence can serve as the promoter to control gene under low-temperature stress with an aim to effectively solve the problem that the soybean low-temperature inducing promoter is not developed yet. The promoter is derived from a promoter sequence of soybean ethylene response factor gene GmERF9, the promoter sequence is named as GmERF0P with a base sequence indicated as SEQ ID No: 1; the sequence contains multiple stress-related cis-acting elements; the cis-elements are respectively: GT-1, BIHD10S, WRKY, MYB, MYC and G-box. The soybean low-temperature inducing promoter is used for inducing cold-resistance gene expression under low temperature.

The invention discloses a newly found DNA sequence of the promoter of the chalcone synthase (chs) gene and the manufacturing method and use for the same. The promoter is used for PCR with a joint from sorbonne, and clones 899bp of the upstream regulation sequence of chs gene by combining the characteristics of the nested PCR, TD PCR, hot-started PCR and two-step PCR. The analysis in the promoter database Plant CARE shows that, the sequence has a basically conservative region TACPyAT which expresses specifically in flowers, and has the main cis-acting elements with which the primary promote is provided such as a transcription initiation related TATA box, a transcription auxiliary CAAT box, a G box and CCAAT etc. The promoter can regulate the specific expression of the target gene in the plant, and can provide an effective promoter element for the constructing the plant expression vector, increase the expression of foreign gene, and minimum the biological energy consumption.

The invention discloses the application of berberine in medicament for treating hepatitis B virus infection. In the invention, adopting an HepG2.2.15 cell model for the in vitro selection of anti-hepatitis B virus medicine, preliminarily selecting the effect of the medicament by measuring the difference of HBeAg and HBsAg expression volumn in supernatant fluid of the cell after administration, andfurther determining the effectiveness of the medicine by studying the inhibitory effect of the HBV DNA reproduction level (ccc DNA) in the HepG2.2.15 cell by the medicament. Meanwhile, the influenceon the reproduction of the hepatitis B virus by the transcriptional level of the medicine is studied according to the activity inhibitory effect to HBV viral promoters by the medicine, and key HBV cis-regulatory elements sensitive to the antivirus medicine are found. A pivotal cellular signal transduction pathway possibly participating the transcription reproduction for inhibiting viruses by the medicine is analyzed and verified on the basis, the antivirus molecular mechanism of the medicament is further studied, and the medicament is indicated to be developed and applied to clinical treatmentas an anti-hepatitis B effective medicament.

The invention discloses a subsequence of Ammopiptanthus mongolicus PeNAC1 gene 1217bp promoter, which is named as PeNAC1P. A PLACE database can be used for analyzing and predicting cis acting elements which are contained in PeNAC1P and are relevant to the induction of drought, high salts, ABA, GA, JA, SA, MeJA and other hormones, elements relevant to bacterial and salts, cis elements responding to calcium ions and cis elements participating in the sugar signal transfer. Analyzed by a transient expression experiment, the activity of the PeNAC1P promoter is induced by the salts, GA and IAA. The Ammopiptanthus mongolicus PeNAC1P promoter provided by the research belongs to an induction type promoter and provides an alternative promoter for generating a normal tolerant transgene plant on the basis of researching the function.

The present invention discloses a method for identifying banana aquaporin gene promoter transcription factors. The method comprises the following steps: S1, cloning and element analysis of a MaPIP1;1promoter are conducted: amplification is conducted according to primers of existing genome designed promoters to an amplification length of 1,362 bp, cis-acting elements are analyzed by plant care andPLACE, and results show that there are 72 cis-acting elements in the 1,362 bp promoter sequence. A core region of the MaPIP1;1 promoter is recognized by a yeast one-hybrid method, the promoter core region M-P2 is used as a bait segment to construct a bait vector, an action mechanism of a MaPIP1;1 gene against drought stress is clarified, and the transcription factors directly binding with the MaPIP1;1 promoter under the drought stress condition are directly identified in plants for the first time.

The invention relates to a rice root tip specific expression promoter Pro-Os04g24469 and application thereof. The promoter has a nucleotide sequence shown as SEQ ID NO:1 or a nucleotide sequence which is derived by replacing or deleting one or more nucleotide from the nucleotide sequence and/or adding one or more nucleotide into the nucleotide sequence and has the same function as the nucleotide sequence shown as SEQ ID NO:1. The invention also provides a carrier, a transgenic cell line and engineering bacteria which contain the promoter and provides a transgenic line with the promoter. The invention provides the application of the rice root tip specific expression promoter Pro-Os04g24469 in regulating and controlling downstream genetic expression. The rice root tip specific expression promoter Pro-Os04g24469 has the beneficial effects that the new rice root tip specific expression promoter is provided for the first time, a foundation is laid for further analysis on cis acting element of root specific expression in following steps, and a new promoter element can also be provided for plant root system development and transformation and reconstruction of root configuration as well as plant stress resistance gene engineering and study on genetic improvement.

The invention discloses a defective slow virus carrier form HIV-1 virus, which comprises the following steps: using cis-form action element of HIV-1 genom and slow virus carrier pLXB from sequence of reaction type protein as initial carrier; obtaining pLXB-Sin by linking up with initial carrier and pHit-Sin carrier of removed U3 district 3íõ-LTR fragment; inserting WPRE element and EGFP; obtaining pLenti-GFP. The invention possesses infected non-cleavage cell, cleavage cell and goal DNA sequence.