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Cold region hybrid japonica rice breeding method

A technology of hybrid japonica rice and cold regions, applied in the field of hybrid rice genetics and breeding, can solve the problems of less restoration resources, difficulty in large-scale promotion and application, and low breeding efficiency

Inactive Publication Date: 2017-01-18
HUNAN HYBRID RICE RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the breeding technology, hybrid rice can be divided into "three-line method" hybrid rice and "two-line method" hybrid rice; at present, "three-line method" hybrid japonica rice in cold regions is facing problems such as few restoration resources and low breeding efficiency, and it is difficult to The area is popularized and applied, and the "two-line method" has free combination and high breeding efficiency, which is expected to bring new opportunities for the cultivation of new combinations of strong dominant hybrid japonica rice

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Examples

Experimental program
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Effect test

Embodiment 1

[0044] Example 1 Breeding of Kongyu 131S, a japonica anti-thermosensitive male sterile line in cold regions

[0045] 1) With Yannong S containing the anti-thermo-sensitive sterile gene as the female parent and Kongyu 131 as the male parent, 143 F1 seeds were obtained by hybridization; the F1 was planted in the Hainan South Breeding Base, the pseudo-hybrids were removed, and the rest were harvested to obtain F2.

[0046]2) Plant F2 in the experimental base of Yanjiagang Farm in Harbin, single planting, the number is 11,000 plants, and observe the fertility at the peak flowering stage. If it is suspected that it is a single plant that is indica-japonica cross sterile, then microscopic examination is performed to confirm the fertility, and the exposed stigma is screened Ten completely sterile individual plants of the partial japonica type with a high rate were cut and regenerated.

[0047] 3) Using the completely sterile single plant regenerated by cutting the stems as the femal...

Embodiment 2

[0053] Example 2 Breeding of 131S / 14-280 Hybrid Combination 131S / 14-280 with Strong Japonica Type in Cold Region

[0054] 1) Analyze the pedigree of conventional rice varieties in Heilongjiang Province, collect other varieties or lines except those containing Kongyu 131, and number them uniformly. A total of 2309 materials were collected.

[0055] 2) Using Kongyu 131S as the female parent and 2309 collected materials as the male parent, carry out hybridization testing and mating, with at least 100 hybrids for each combination.

[0056] 3) Preliminary test, the obtained hybrid grains were planted in Yanjiagang Farm, Heilongjiang Province, and the control Longdao 5 field layout was planted at intervals of 500. Compared with the control, the growth period, seed setting rate, disease resistance, and high yield 2269 combinations were eliminated according to the traits, and hybridization was performed again according to the numbers of the remaining 40 combinations, and retesting was...

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Abstract

The invention relates to the field of hybrid rice genetic breeding, and especially relates to a cold region hybrid japonica rice breeding method. The method comprises the following steps: 1, carrying out backcross breeding with Yannong S carrying out an inverse temperature-sensitive sterile gene as a donor parent and cold region japonica rice R1 with excellent comprehensive properties as a recurrent parent, and cultivating to obtain an inverse temperature-sensitive sterile line R1S reserving the basic characteristics of the R1; 2, carrying out massive crossing hybridization combination with the R1S as a female parent and cold region japonica rice R2 with excellent comprehensive properties as a male parent to obtain R1S / R2; and 3, evaluating the comprehensive properties of the R1S / R2 with test sample species in the same ecologic region, and screening to obtain cold region hybrid japonica rice, wherein the comprehensive properties comprise but not be limited to one or more of the output, the cold resistance, the disease resistance and the rice quality, and the cold region japonica rice R2 and the cold region japonica rice R1 have genetic differences. The cold region hybrid japonica rice breeding method has the characteristics of high critical sterility inducing temperature, stable hereditary potency, high yield, high quality, good cold and disease resistance, and inverse temperature-sensitive sterility.

Description

technical field [0001] The invention relates to the field of genetic breeding of hybrid rice, in particular to a method for breeding hybrid japonica rice in cold regions. Background technique [0002] Rice areas in cold regions (such as Heilongjiang Province) mainly plant conventional rice, and the genetic relationship of the varieties is relatively close. The yield potential of conventional rice has encountered a bottleneck, and new breakthroughs in breeding ideas are needed. Hybrid rice can make full use of the advantages between two parents with different backgrounds, and generally increases yield by more than 8% compared with conventional rice. According to the breeding technology, hybrid rice can be divided into "three-line method" hybrid rice and "two-line method" hybrid rice; at present, "three-line method" hybrid japonica rice in cold regions is facing problems such as few restoration resources and low breeding efficiency, and it is difficult to The area is populari...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 高扬段美娟徐秋生解保胜孟昭河裴又良谭炎宁张景龙田红刚臧家祥步金宝宋微
Owner HUNAN HYBRID RICE RES CENT
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