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High-yield Tricholoma matsutake sing mutagenesis strain and breeding method thereof

A technology for mutagenic strains and matsutake mushrooms is applied in the field of high-yield matsutake mutagenic strains, and can solve problems such as high price, obstacles to the development of the matsutake industry, and difficulties in artificial cultivation.

Active Publication Date: 2015-11-11
北京中京丰创科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, matsutake mainly comes from wild picking, and artificial cultivation is very difficult. A large amount of market demand has caused the depletion of matsutake resources, the destruction of the ecological environment and the high price, which has brought great obstacles to the development of matsutake industry.

Method used

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  • High-yield Tricholoma matsutake sing mutagenesis strain and breeding method thereof
  • High-yield Tricholoma matsutake sing mutagenesis strain and breeding method thereof
  • High-yield Tricholoma matsutake sing mutagenesis strain and breeding method thereof

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Experimental program
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Effect test

Embodiment 1

[0033] Mutation and Breeding Methods of Matsutake Mutant Strain

[0034] (1) Matsutake mutagenesis

[0035] Inoculate the starting strain of the matsutake strain with the preservation number CGMCC5.793 after activation and inoculate it on a PDA slant, cultivate it in a 26oC incubator for 6 days, inject pH6.0 phosphate buffer into the slant medium, and then filter it with 4 layers of gauze. Dilute the spore concentration to 107 / mL for mutation breeding. Weigh 10 mg of nitrosoguanidine into a petri dish with a diameter of 7 cm, add a little acetone as a co-solvent, add 9 mL of sterile phosphate buffer (pH=6.0), turn on the magnetic stirrer and stir slowly, and absorb 1 mL after NTG is fully dissolved The bacterial suspension was put into a plate to start mutagenesis (the final concentration of nitrosoguanidine was 1 mg / mL). After mutagenesis for 2min, 5min, 10min, 15min and 20min respectively, take 0.3mL of bacterial suspension in 2.7mL of sterile distilled water and seriall...

Embodiment 2

[0045] Optimization of Fermentation Medium for Matsutake Mutagenic Strains

[0046] 1. Establish the expected function

[0047] The dry weight of bacteria, intracellular polysaccharides, adenosine, and mannitol were used as the investigation indicators to optimize the matsutake fermentation process, and the expectation function was used to integrate multiple investigation indicators into one investigation index expectation value (D), and each investigation index ( Response value) are converted into dimensionless expected values ​​according to formula (1). The balance scale d of the expected function ranges from 0 to 1. When d=0, it means that the response value seriously deviates from the target value, and when d=1, it means that the response value is close to the target value. . d increases as the expected response increases.

[0048] (1)

[0049] is the response value of the i-th inspection index, Li is the expected response value of the i-th inspection index cann...

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Abstract

The present invention discloses a Tricholoma matsutake sing mutagenesis strain and a breeding method thereof. According to the present invention, a new high-yield Tricholoma matsutake sing strain is obtained through chemical mutagenesis screening separation, wherein the preservation number is CCTCC N0:2014671, the mycelium dry weight is increased by 69.68% compared with the original strain, the adenosine content is increased by 33.96%, the polysaccharide content is increased by 75.72%, the protein content is increased by 35.17%, and the mannitol content is increased by 52.8%; and after subculturing is performed on the new Tricholoma matsutake sing strain more than or equal to 10 times, no degeneration is generated, and the genetic stability is provided. The present invention further discloses an optimal fermentation culture medium of the Tricholoma matsutake sing mutagenesis strain.

Description

technical field [0001] The invention relates to a high-yield matsutake mutagenic strain, which is a new strain variety. The invention also provides a cultivation method of the strain, which belongs to the technical field of biological fermentation engineering. Background technique [0002] Matsutake (TricholomamatsutakeSing.) belongs to Basidiomycetes, Basidiomycetes, Agaricaceae, Tricholomaceae, Tricholoma genus, also known as Shan mushroom, pine mushroom, synbacterium, Taiwan fungus and pine mushroom. Matsutake has a long history of use and is widely distributed around the world. Its meat is white and tender, fine in texture, and has a strong special aroma. It is rich in nutrients and contains active ingredients such as protein, amino acid, multivitamins, carbohydrates, and minerals. It is a valuable wild fungus . The main biologically active substances of matsutake are polysaccharides, polypeptides and sterols. Studies on pharmacological effects have shown that matsutake...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/01C12R1/645
Inventor 王迪李佺孟庆繁王美羽滕利荣逯家辉黄晨玮张洋权宇彤
Owner 北京中京丰创科技有限公司
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