Escherichia coli recombination strain for producing levodopa as well as construction method and application thereof

Abstract

The invention provides an escherichia coli recombination strain T002 for producing levodopa as well as a construction method and application thereof. According to the escherichia coli recombination strain, expression of 3-dehydrogenase shikimate dehydrogenase (aroE) is up regulated to enhance enzyme activity of 3-dehydrogenation shikimate dehydrogenase, and the levodopa can be further produced. The strain can produce the levodopa by utilizing glucose inorganic salt culture medium production, culture medium cost is reduced, and production cost of the levodopa is reduced. Furthermore, the recombination strain has the advantages of no plasmid and stable hereditary.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to recombinant Escherichia coli for producing levodopa and its construction method and application. Background technique

[0002] Levodopa (L-DOPA), also known as 3-hydroxyl-L-tyrosine, is an amino acid derivative with remarkable effects in medicine, hygiene, health care and beauty. Since 1908, it has been widely used clinically all over the world. Levodopa is currently the most effective drug for treating parkinsonism. It enters the brain tissue through the blood-brain barrier and plays a role. It is suitable for primary parkinsonism and non-pharmaceutical parkinsonia syndrome. At the same time, it has curative effects on stiffness, dyskinesia, salivation, and arterial crisis. Levodopa is an intermediate in the formation of catecholamines from tyrosine, the precursor of dopamine. About 95% of L-dopa absorbed into the blood is decarboxylated by dopa decarboxylase in peripheral tiss...

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