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SNP marker for analyzing rice genetic diversity and identifying varieties, primers and application

A technique assisted by genetic diversity and molecular markers, applied in the field of molecular biology, can solve problems such as lack of correlation, difficulty in distinguishing near-isogenic lines or functional substitution lines, and poor correlation of agronomic traits, achieving good versatility and low cost. The effect of control

Active Publication Date: 2018-04-03
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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Problems solved by technology

However, due to the poor or lack of correlation between SSR markers and the agronomic traits of varieties, it is difficult to distinguish near-isogenic lines or functional substitution lines. For such varieties, SNP markers (functional markers) of related characteristic functional genes are needed to Differentiate from the original species

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  • SNP marker for analyzing rice genetic diversity and identifying varieties, primers and application
  • SNP marker for analyzing rice genetic diversity and identifying varieties, primers and application
  • SNP marker for analyzing rice genetic diversity and identifying varieties, primers and application

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with specific examples, but should not be construed as a limitation of the present invention. The experimental methods in the following examples, unless otherwise specified, are conventional methods, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0024] 1 Test materials and methods

[0025] 245 samples of the main hybrid rice and its parents in the middle and lower reaches of the Yangtze River were collected (Table 2), including 157 samples of conventional species / parents, 38 samples of two-line hybrids, and 50 samples of three-line hybrids. The DNA of individual plants (10 individual plants of each variety) was extracted by CTAB method, and stored at -20°C as experimental materials for the next step.

[0026] Table 2 245 rice samples

[0027]

[0028]

[0029]

[0030] 2. Rice functional gene screeni...

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Abstract

The invention belongs to the technical field of molecular biology and particularly discloses an SNP marker for analyzing rice genetic diversity and identifying varieties, loci of the SNP marker in rice, primers obtaining the SNP marker as well as related application. The SNP marker has good generality and does not need large-sized equipment instrument. The SNP marker disclosed by the invention canbe used for accurately distinguishing genotypes of to-be-detected varieties, genetic difference among the varieties and difference among the loci with close genetic distance; after the primer of certain locus is designed, a plurality of individuals can be researched for the locus, so that the cost is well controlled.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a SNP marker for analyzing rice genetic diversity and identifying varieties and its application. Background technique [0002] Rice is an important food crop in my country, and hybrid rice has made great contributions to food security in my country and the world with its remarkable yield advantage. The promotion and use of new varieties has played an extremely important role in ensuring my country's food supply and meeting people's diverse needs. From 2001 to 2015, 6,310 rice varieties passed provincial-level and above approvals across the country. Effectively distinguishing and identifying these variety materials has important practical significance for rice variety breeding, testing, quality and market management, and property rights protection. [0003] Varieties of morphological differences and differences in characteristics, in essence, are caused by ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 秦瑞英许学马卉倪金龙李莉汪秀峰李浩杨亚春宋风顺王钰杨剑波
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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