Primer for identifying coilia ectenes and coilia brachygnathus and molecular biological method

Abstract

The invention provides a primer for identifying coilia ectenes and coilia brachygnathus and a molecular biological method. The primer contains an amplified primer group and an extended primer group. A nucleotide sequence of the amplified primer group is shown as SEQ ID NO:1-16, a nucleotide sequence of the extended primer group is shown as SEQ ID NO:17-24. The primer combination can be effectively used for identifying the coilia ectenes and the coilia brachygnathus. The molecular biological method comprises the steps that a to-be-tested coilia ectenes and coilia brachygnathus SNP locus area nucleic acid sequencing library is established, sequencing is conducted on the library to obtain a sequencing result; based on the sequencing result, a coilia ectenes and coilia brachygnathus SNP locus area nucleic acid sequence is determined; then Structure2.3.2 software is utilized to draw a column diagram of population genetic differences and a column diagram of individual genetic differences, and species are judged by utilizing analysis. The method includes simple and convenient steps and is accurate, efficient and low in cost.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer and a molecular biology method for identifying the common anchovy and the short-jawed anchovy. Background technique [0002] Anchovy is an important fishery resource in the Yangtze River, and it is also a rare and precious aquatic product unique to the Yangtze River. In the Yangtze River, there is also another species of short-jawed anchovy that is very similar in shape. The taste and value of short jawed anchovy are quite different from those of sword anchovy. On the market, individuals of short-jawed anchovy are often used to pass off as knife anchovy for sale, and because the main difference between the two is the lower jaw, the difference is small and difficult to distinguish, and it is often more difficult to distinguish due to the damage of gillnet fishing; on the other hand, due to the current knife Artificial breeding of anchovy has not yet been realized, and breed...

AI Technical Summary

Problems solved by technology

On the market, individuals of short-jawed anchovy are often used to pass off as knife anchovy for sale, and because the main difference between the two is the lower jaw, the difference is small and difficult to distinguish, and it is often more difficult to distinguish due to the damage of gillnet fishing; on the other hand, due to the current knife Artificial breeding of anchovy has not yet been realized, and breeding needs to rely on seedlings caught in the Yangtze River. However, the seedlings of sword anchovy and short-jawed anchovy often appear mixed in the same area and are difficult to distinguish, and they often have to wait until they are raised to larger individuals before they can be distinguished and removed. Increased the cost of farming

[0003] In the prior art, the mitochondrial DNA technology is usually used to screen the genes of the anchovy, anchovy, and anchovy, but this method mainly has the following defects: the first choice is that the anchovy and anchovy are quite different from each other and are easier to use. Mitochondrial gene sequence is used to distinguish the two species. As the two are sister species, the differentiation time is short, the mitochondrial difference is not obvious, and there is even a certain genetic introgression phenomenon. Therefore, it is not suitable to use the mitochondrial genome sequence to distinguish between the two

Secondly, mitochondria are maternally inherited, and their sequences can only reflect the characteristics of their maternal ancestors. In the Yangtze River, the two have a common distribution area and genetic introgression, which may cause errors in identification

At the same time, the genetic background of the short-jawed anchovy in some water systems is closer to that of the Yangtze anchovy, so only the mitochondrial gene sequence analysis is applicable, and it is difficult to distinguish their species affiliation

On the other hand, if the sequence data of mitochondria is small, the amount of information is small, and if the sequence data is large, the amount of information is large, and the cost is also relatively high

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