47results about How to "Screening results are accurate" patented technology

The invention discloses a method for screening a feature peptide fragment for quantitatively detecting protein in food, and belongs to the technical field of protein quantitative detection. The screening method comprises the following steps: (1) comparing and analyzing matching score and interference number corresponding to a to-be-detected protein theoretical enzymolysis fragment by utilizing BLAST; (2) analyzing a mass spectrum response value and fragment abundance of a target peptide fragment through high performance liquid chromatography-tandem mass spectrometry; (3) calculating the enzymolysis rate of the target peptide fragment when the enzymolysis is conducted for 1 to 3 hours by utilizing a fitted curve; (4) calculating the recovery rate and the precision degree of the target peptide fragment. The feature peptide fragment suitable for protein accurate quantification is comprehensively screened from the aspects of specificity, mass spectrum characteristic, enzymolysis characteristic, accuracy, stability and the like and by utilizing various methods in a combined way; the screened feature peptide fragment can meet the quantitative detection requirement of high performance liquid chromatography-tandem mass spectrometry on protein in food matrix, and realizes protein quantitative detection requirement with high sensitivity, high precision degree and excellent reproducibility.

The invention discloses a nondestructive stem-removal picking bionic gecko distinguishable pick-up and screening vehicle of a crabapple and belongs to the technical field of crabapple picking machines. The vehicle comprises a light moving mechanism, a flexible fruit stem clamping and nondestructive conveying picking mechanism, an extension steering device, a nondestructive protection stem-removaldevice, a simulating gecko foot bristle distinguishable pick-up delivery mechanism, a turnover cleaning purification mechanism and a rotary sliding-down screening mechanism, wherein the turnover cleaning purification mechanism, the extension steering device and the rotary sliding-down screening mechanism are arranged on the light moving mechanism; the simulating gecko foot bristle distinguishablepick-up delivery mechanism is arranged at the front end of the turnover cleaning purification mechanism and the light moving mechanism; the flexible fruit stem clamping and nondestructive conveying picking mechanism is arranged on the extension steering device; and the nondestructive protection stem-removal device is arranged on the flexible fruit stem clamping and nondestructive conveying pickingmechanism. The nondestructive stem-removal picking bionic gecko distinguishable pick-up and screening vehicle of the crabapple achieves dislocation stirring for nondestructive picking, absorption pick-up, nondestructive cleaning and roll screening.

The invention relates to a consistency screening method for high-rate lithium titanate unit cells. The screened lithium titanate cells have high consistency under a high-current discharge working condition. The method comprises the following steps of adjusting the cells to a specific charge state and storing the cells at elevated temperature for a short time so as to quickly screen out the cells with large self-discharge; and then carrying out a specific single-point high-frequency and low-frequency alternating-current impedance test on the cells stored at the elevated temperature so as to directly reflect the ohmic internal resistance and the polarization internal resistance of the cells. According to the method, self-discharge and dynamic internal resistance of the cells are rapidly andaccurately evaluated while the parameters of conventional discharge capacity, open-circuit voltage, constant voltage time, static internal resistance and the like are taken into consideration, and thescreening result is more comprehensive, accurate and effective; and according to the scheme, the consistency screening of the cells can be completed within one day, the screening cycle of the cells can be greatly shortened, the operation is simple and convenient, the energy consumption is low, and the method is suitable for large-scale batch application of an automatic production line.

The invention relates to a screening method of dominant hybridization species of German mirror carps and discloses a screening method of the dominant hybridization species of carps. The invention provides an effective screening method of the dominant hybridization species of the German mirror carps so as to ensure the genetic diversity of hybrids thereof, thus reducing the workloads, shortening the breeding period, decreasing the breeding cost and improving the success rate of selective breeding. The screening method includes the following steps: 1. morphological marker; 2. microsatellite molecular maker; and 3. data analysis. The method screens the dominant hybridization species of the German mirror carps by combining the morphological marker and the microsatellite molecular marker. The genetic differences between the species are dually tested and the screening results are more accurate.

The invention discloses a false matching removal method based on superpixel motion statistics. The method comprises the following steps: carrying out feature extraction, description and matching on two images to be matched; Segmenting the to-be-matched images I1 and I2 by using an improved superpixel segmentation algorithm to obtain two superpixel mark graphs; And establishing a superpixel motionstatistical model based on the superpixel marker map, and realizing automatic screening of registration feature points of the non-rigid deformation image through the model. According to the method, asuper-pixel segmentation strategy is adopted to replace simple rectangular grid division, super-pixel blocks obtained through segmentation are tightly connected in space, colors and textures in singlesuper-pixel blocks are kept consistent, a segmentation result better follows the moving edge of an object, and therefore it is guaranteed that feature points in super-pixels have the same or consistent moving tendency.

The invention belongs to the technical field of screening of agricultural germplasm resources, and particularly relates to a screening method for a selenium-enriched rice variety. According to the method, preliminary screening is performed at first through indoor cultivation, so that the blindness of the subsequent field screening test is reduced; South Henan black glutinous rice is used as a contrast material, so that the screening process is simple and feasible. In the indoor screening process, a selenium-enriched culture medium is used to provide a uniform and stable selenium source for rice seedlings; the accuracy of variety screening can be substantially improved; seeds are started to be cultivated in the initial stage of germination and growth; nutrition is mainly supplied by the seeds to the seedlings; the culture medium is relatively easy to prepare, and the screening method is easy to apply.

The invention discloses an automatic screening apparatus and an automatic screening method for clone bacterial strains. The apparatus comprises a three-dimensional electronic control motion platform for placing a culture dish and changing a spatial position thereof, the control side of the three-dimensional electronic control motion platform is connected with corresponding control side of a central management system; and the periphery of the three-dimensional electronic control motion platform is provided with a high resolution digital camera, a binocular stereoscopic visual sensor system, a measuring characteristic optical projection system and an optical illumination system, wherein the control side of the measuring characteristic optical projection system is connected with corresponding control side of the central management system; and the control sides of the high resolution digital camera and the binocular stereoscopic visual sensor system are connected with corresponding control sides of the central management system via an image acquisition control system. The method realizes automatic screening through monitoring logarithmic phase of the growth of the clone bacterial strains. The method realizes rapid and automatic screening for the clone bacterial strains through the apparatus; the screening results are accurate; and the screened clone bacterial strains are undamaged.

The invention discloses a glass fiber cloth cover black and grey detection method. The detection method comprises the following steps: firstly, collecting a picture of a glass fiber cloth cover; converting the color image data f into first grayscale image data g0; equally dividing the first grayscale image data g0 into m first areas g0m; performing filtering processing on the plurality of first areas in the picture through a preset template, and performing arrangement to obtain second grayscale image data gt; checking the ratio of the two pieces of grayscale image data and a preset standard, and screening out boundary points; and extracting all boundary points to further judge whether the boundary points are black gray. A color picture is subjected to gray processing and then is divided into multiple parts according to a preset width, the initial gray picture is divided according to the preset width and then is subjected to respective filtering processing, so that the screening result of a final black-gray area can be more accurate, and the gray values of the image before and after filtering processing are checked according to a preset standard to screen out boundary points. And whether the cloth surface is black gray is judged according to the continuity of the plurality of continuous boundary points in the coordinate system.

The invention discloses a pile brace supporting structure screening method based on the sacrifice yield rate. The pile brace supporting structure screening method comprises the following steps that 1,a feasible region of a foundation pit pile brace supporting structure is established; 2, a noise factor set is established; 3, FLAC<3D> software is utilized to simulate the foundation pit excavationprocess, and a system response result is obtained; 4, robustness measurement indexes of the foundation pit pile brace supporting structure are calculated; 5, the cost of the foundation pit pile bracesupporting structure is calculated; 6, a Pareto leading edge is drawn according to the cost and the robustness measurement indexes; and 7, a knee point of the Pareto leading edge is obtained by applying the sacrifice yield rate, that is, the optimal foundation pit pile brace supporting structure designing scheme is obtained. By screening points of the Pareto leading edge, the influences of the similar points on knee point selection are weakened, the knee point is multiply screened to avoid trapping in the dilemma of local optimum, dependence on shape and curve fitting of the Pareto leading edge is small during knee point screening is small, and the screening result is accurate and reliable.

The invention is suitable for the technical field of grape screening, and provides a grape particle screening machine for grape wine production and processing, the grape particle screening machine comprises a transmission assembly, an oscillation screening assembly and a linkage assembly, a feeding pipe can gradually put grape particles onto the oscillation screening assembly, then the oscillation screening assembly is driven, and the grape particles are conveyed to the transmission assembly in the operation process of the oscillation screening assembly. When the grape grains are conveyed, the conveying assembly is driven through the linkage assembly, so that the grape grains are pushed to be conveyed step by step, after the conveying assembly finishes running each time, the oscillating screening assembly oscillates once, grapes are classified according to the grape grains, the grape grains fall into the storage box according to specifications and sizes and are separated through the partition plates, and meanwhile, the grape grains are conveyed through the conveying assembly. The sensor can detect whether grapes fall or not, when no grapes fall into the storage box for a long time, it can be judged that the grape screening work is completely completed, at the moment, the sensor can automatically close the vibration screening assembly, operation is easy, the automation degree is high, and the screening result is accurate.

The invention provides a train tail screening method and device and a storage medium, and the method comprises the steps of determining whether a to-be-screened train meets a preset tail screening condition, and enabling the preset tail screening condition to be associated with at least one of train suspension, axle counting state collection delay and train-ground communication delay, wherein the train suspension and/or the train-ground communication delay are/is associated with a distance judgment condition included in the preset tail screening condition, and the train-ground communication delay is associated with a section idle judgment condition included in the preset tail screening condition; when the to-be-screened train meets the preset tail screening condition, determining that tail screening of the to-be-screened train is passed; and when the to-be-screened train does not meet the preset tail screening condition, determining that tail screening of the to-be-screened train is not passed. At least one of train suspension, axle counting state acquisition delay and train-ground communication delay is considered in the tail screening condition, so that the train tail screening result is more accurate, and train tail screening is more reasonable and safer.

The invention discloses a method and device for screening similar statements in a database. The method comprises the steps that semantic vectors and semantic vectors of multiple target extension statements of a target standard statement are utilized to expect to screen all the target extension statements to obtain extension statements to be subjected to quality inspection; Calculating the similarity between the semantic vector of each extended statement to be subjected to quality inspection and the semantic vectors of other extended statements in the database; Wherein the semantic vector is obtained through a semantic measurement model; And based on the similarity and a first preset screening condition, screening each extended statement to be subjected to quality inspection and each otherextended statement to obtain similar extended statements to be subjected to quality inspection and corresponding similar other extended statements. Therefore, only the target extension statement needing quality inspection is screened as the to-be-quality-inspected extension statement, and the number of the to-be-quality-inspected extension statements is reduced; Compared with a screening result obtained through manual screening, the automatic similar statement screening is more perfect and accurate, manpower and time are saved, and the screening efficiency is improved.

The invention belongs to the field of germ screening and particularly relates to a system and a method for screening a model infected with arcanobacterium pyogenes or corynebacterium pseudotuberculosis. The system comprises an elisa plate, a heater, a reagent, a database, an elisa instrument and a data comparison output module, wherein the elisa plate comprises a polystyrene/polyvinyl chloride material label plate which comprises 96 holes or 48 holes; the reagent comprises an antigen coating solution, an enzyme-labeled antibody, a developing solution and a germ infection model or immune model serum; the database generates absorbance of different antibody amounts for a healthy or infected or immunized animal model, and the absorbance can be imported and deleted; and the wavelength of the microplate reader is set to be 450 nm. The screening system provided by the invention is complete in database, accurate in screening result, capable of quickly screening out the types of the bacteria infecting the bacteria model, simple and convenient.

The invention provides a protein biomarker for sepiella japonica in the senescence process and belongs to the technical field of genetic engineering. The protein biomarker comprises a mitochondrial ATP/ADP (adenosine triphosphate/adenosine diphosphate) carrier protein SLC25A4S, has relative abundance of 1.82*10<7> before sexual maturation and oviposition, and has relative abundance of 0 during oviposition and articulo mortis after oviposition. A screening method of the protein biomarker comprises the following steps: extracting optic gland protein samples from individuals of sepiella japonicaat different reproductive stages; performing enzymolysis on the protein samples by using trypsin; analyzing the protein samples after enzymolysis by using liquid chromatogram-mass spectrum combination; and performing protein differential expression analysis, gene ontology analysis, signal pathway analysis and protein homologous cluster analysis through LC-MS (liquid chromatogram-mass spectrum) data processing. Through proteomic analysis and screening on the optic gland protein samples of the sepiella japonica at different reproductive stages, potential biomarkers can be obtained.

The invention belongs to the field of plant endophyte separation and microbial fermentation engineering, and particularly relates to a method for separating metabolizable cephalotaxus sinensis alkaloid strains. According to the method, roots, stems and leaves of perennial cephalotaxus sinensis are selected as explant; the explant is cut to strips, sterilized and then placed into a culture medium for culturing so as to produce cephalotaxus sinensis symbiotic bacteria; and then the cephalotaxus sinensis symbiotic bacteria are separated according to different strains and cultured and fermented respectively, and the cephalotaxus sinensis symbiotic bacteria are detected after fermentation broth is concentrated so as to determine whether the cephalotaxus sinensis symbiotic bacteria are metabolizable cephalotaxus sinensis alkaloid strains. In a fermentation broth preparation process, second batch of strains are inoculated after metabolic organisms are generated by the strains, the metabolic organisms generated by the previous batch of strains provide nutrients for the second batch of strains, so that growth of the second batch of strains is promoted greatly, the biological activity is improved simultaneously, a large amount of fermentation broth is generated accordingly, and the accuracy is improved while strain screening is facilitated.

The invention discloses a method for screening salt-tolerant day lily varieties based on glyoxalase activity. The method comprises the following steps: taking tender day lily leaves; putting into liquid nitrogen and crushing into fine powder; adding phosphate buffer, after oscillating and shaking up, centrifuging at low temperature, then taking the supernatant, dividing into two equal parts, one part is used for determining the protein content, the other part is used for determining the activity of glyoxalase, the activity of glyoxalase is calculated according to the consumption of the substrate methyl glyoxalase and the protein content per minute, and the plant with high glyoxalase activity is salt-tolerant. The salt-tolerant day lily variety is screened by measuring the activity of glyoxalase in vivo. Meanwhile, in order to avoid errors caused by fresh weight, the protein content in vivo is firstly measured, and the salt tolerance of the day lily is screened according to the glyoxalase activity of unit protein. Therefore, the method provided by the invention has the advantages of short period, accurate screening result, low cost and the like, and is of great significance to day lily salt resistance research and breeding.