A screening method for selenium-enriched rice varieties

A screening method and variety technology, applied in the field of agricultural germplasm resource screening, can solve the problems of long cycle and low accuracy, and achieve the effects of improving accuracy, accurate screening results, and easy application of screening methods.

Active Publication Date: 2020-10-16
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome the defects of low accuracy and long cycle in the screening process of rice germplasm resources in the prior art, thereby providing a screening method for selenium-enriched rice varieties

Method used

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  • A screening method for selenium-enriched rice varieties
  • A screening method for selenium-enriched rice varieties
  • A screening method for selenium-enriched rice varieties

Examples

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Embodiment 1

[0022] The present embodiment provides a screening method for selenium-enriched rice varieties, comprising the following specific steps:

[0023] 1) Select the seeds of Huanghuazhan, Zhongzao 35, IR19735, Yangdao No. 6, Wuyanjing, and Xiangwanxian No. 12 with plump and healthy grains to be screened. % ethanol disinfection for 2 minutes, then soaked with 2% sodium hypochlorite for 35 minutes, rinsed with sterile water for 4 times, germinated at 30 °C, and sown in a petri dish pre-configured with selenium-containing medium after the seeds were exposed. grain.

[0024] Wherein, the selenium-containing medium is composed of the following components: sodium selenite 1.5 mg / L, fulvic acid 0.1 mg / L, and agar 8 g / L, prepared with sterile distilled water.

[0025] 2) Put the petri dish in a plant light incubator, the humidity is 70-90%, and the light intensity during the day is 100-120 μmol / m 2 S, the light time is 10 hours, the temperature during the day is 30-32℃, and the temperatu...

Embodiment 2

[0031] The present embodiment provides a screening method for selenium-enriched rice varieties, comprising the following specific steps:

[0032] 1) Select the seeds of the rice cultivars to be screened with plump and healthy seeds of Juba No. 1, Xiangwanxian No. 13, Heishai, Minghui 63, Meixiangxinzhan, Zaonuo, and Ganxiangzhan. As a control, the seeds were sterilized with 75% ethanol for 1 minute, then soaked with 3% sodium hypochlorite for 25 minutes, rinsed with sterile water for 5 times, and germinated at 28°C. 120 grains per petri dish.

[0033] Wherein, the selenium-containing medium is composed of the following components: sodium selenite 0.1 mg / L, fulvic acid 1 mg / L, and agar 6 g / L, prepared with sterile distilled water.

[0034] 2) Put the petri dish in a plant light incubator, the humidity is 70-90%, and the light intensity during the day is 100-120 μmol / m 2 S, the light time is 12 hours, the temperature during the day is 30-32 °C, and the temperature at night is ...

Embodiment 3

[0040] The present embodiment provides a screening method for selenium-enriched rice varieties, comprising the following specific steps:

[0041] 1) Select the seeds of Shuhui 527, R216, Heizhan 96, Jintai Ruanzhan, Heishai, Chunhui 350 and Chishi with plump and healthy grains of the rice varieties to be screened. % ethanol disinfection for 2 minutes, then soaked with 2.5% sodium hypochlorite for 30 minutes, rinsed with sterile water for 5 times, and germinated at 29 °C. grain.

[0042]Wherein, the selenium-containing medium is composed of the following components: sodium selenite 1 mg / L, fulvic acid 0.5 mg / L, and agar 7 g / L, prepared with sterile distilled water.

[0043] 2) Put the petri dish in a plant light incubator, the humidity is 70-90%, and the light intensity during the day is 100-120 μmol / m 2 S, the light time is 11 hours, the temperature during the day is 30-32 °C, and the temperature at night is 25-27 °C.

[0044] 3) cultivate for 6 days, when the rice seedling...

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Abstract

The invention belongs to the technical field of screening of agricultural germplasm resources, and particularly relates to a screening method for a selenium-enriched rice variety. According to the method, preliminary screening is performed at first through indoor cultivation, so that the blindness of the subsequent field screening test is reduced; South Henan black glutinous rice is used as a contrast material, so that the screening process is simple and feasible. In the indoor screening process, a selenium-enriched culture medium is used to provide a uniform and stable selenium source for rice seedlings; the accuracy of variety screening can be substantially improved; seeds are started to be cultivated in the initial stage of germination and growth; nutrition is mainly supplied by the seeds to the seedlings; the culture medium is relatively easy to prepare, and the screening method is easy to apply.

Description

technical field [0001] The invention belongs to the technical field of agricultural germplasm resource screening, and in particular relates to a screening method for selenium-enriched rice varieties. Background technique [0002] As an important trace element in the ecological environment, selenium (Se) has various effects such as anti-aging, anti-cancer and detoxification. The third largest micronutrient health element. The safe intake of selenium recommended by the Health Institute of the Chinese Preventive Medicine Center is 40-240 μg / person / day. The survey report shows that the current adult intake of selenium is about 26.63 μg / day, so it is necessary to further increase the intake of selenium. [0003] Rice is the main food crop in my country. It is an ideal way to supplement selenium by increasing the selenium content of rice and increasing human selenium intake from the diet. Rice is one of the non-Se plants with low selenium content, but the genotypes of its accumu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04
CPCA01H1/04
Inventor 张标金史华新陈庆隆戴廷灿周瑶敏魏益华
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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