Protein biomarker for sepiella japonica in senescence process

A technology of squid mansoni and biomarkers, which is applied in the field of genetic engineering, can solve the problems that there is no squid mansoni, etc., and achieve the effect of low cost, reasonable design and large number of screening

Inactive Publication Date: 2020-01-31
ZHEJIANG OCEAN UNIV
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  • Description
  • Claims
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Problems solved by technology

However, there is no relevant research report on squid mansoni at home and abroad

Method used

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  • Protein biomarker for sepiella japonica in senescence process
  • Protein biomarker for sepiella japonica in senescence process
  • Protein biomarker for sepiella japonica in senescence process

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Embodiment 1

[0041] A screening method for protein biomarkers in the aging process of squid mansoni

[0042] 1. Collection and sampling of experimental animals;

[0043] The three stages of sexually mature squid before spawning, spawning, and dying after spawning were collected from the demonstration base of seedling breeding and cultivation of squid mansoni in Shacheng Town, Fuding City, Fujian Province Individuals were marked as three groups A, B, and C, and 3 individuals were collected from each group, and their optic glands were selected, numbered A1~A3 (before spawning); B1~B3 (during spawning); C1~C3 ( near death). The optic gland is an important organ related to reproductive regulation in cephalopods. During the aging process of squid mansoni, the abundance of some proteins in the optic gland will change significantly before aging. It can be used as a potential biomarker in the aging process of squid mansoni.

[0044] 2. Protein extraction;

[0045] The protein was extracted usi...

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Abstract

The invention provides a protein biomarker for sepiella japonica in the senescence process and belongs to the technical field of genetic engineering. The protein biomarker comprises a mitochondrial ATP/ADP (adenosine triphosphate/adenosine diphosphate) carrier protein SLC25A4S, has relative abundance of 1.82*10<7> before sexual maturation and oviposition, and has relative abundance of 0 during oviposition and articulo mortis after oviposition. A screening method of the protein biomarker comprises the following steps: extracting optic gland protein samples from individuals of sepiella japonicaat different reproductive stages; performing enzymolysis on the protein samples by using trypsin; analyzing the protein samples after enzymolysis by using liquid chromatogram-mass spectrum combination; and performing protein differential expression analysis, gene ontology analysis, signal pathway analysis and protein homologous cluster analysis through LC-MS (liquid chromatogram-mass spectrum) data processing. Through proteomic analysis and screening on the optic gland protein samples of the sepiella japonica at different reproductive stages, potential biomarkers can be obtained.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a protein biomarker in the aging process of squid mansoni. Background technique [0002] Sepiella japonica belongs to the phylum Molluscs, Cephalopoda, Order Sepia, Sepiaceae, and the genus Sepia. It is an important economic species in the East China Sea and has high edible, medicinal and economic value. The life cycle of Squid mansonii is generally only one year, and its resources are composed of the supplementary groups of that year. However, since the mid-1970s, due to the deterioration of environmental conditions and overfishing by fishermen, the resources of squid mansoni in my country have been greatly damaged and even tended to be exhausted. In order to quickly restore the resources of squid mansoni in our country and carry out resource restoration work, it is important to increase the intensity of artificial breeding of squid mansoni, implement se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435G01N30/02G01N30/06G01N33/68
CPCC07K14/43504G01N30/02G01N30/06G01N33/68G01N2333/43504
Inventor 郭宝英许中天祁鹏志杨槐
Owner ZHEJIANG OCEAN UNIV
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