Automatic screening apparatus and automatic screening method for clone bacterial strains

Abstract

The invention discloses an automatic screening apparatus and an automatic screening method for clone bacterial strains. The apparatus comprises a three-dimensional electronic control motion platform for placing a culture dish and changing a spatial position thereof, the control side of the three-dimensional electronic control motion platform is connected with corresponding control side of a central management system; and the periphery of the three-dimensional electronic control motion platform is provided with a high resolution digital camera, a binocular stereoscopic visual sensor system, a measuring characteristic optical projection system and an optical illumination system, wherein the control side of the measuring characteristic optical projection system is connected with corresponding control side of the central management system; and the control sides of the high resolution digital camera and the binocular stereoscopic visual sensor system are connected with corresponding control sides of the central management system via an image acquisition control system. The method realizes automatic screening through monitoring logarithmic phase of the growth of the clone bacterial strains. The method realizes rapid and automatic screening for the clone bacterial strains through the apparatus; the screening results are accurate; and the screened clone bacterial strains are undamaged.

Description

technical field [0001] The invention relates to an automatic screening device and method for cloning bacterial strains, belonging to the technical field of microbial measurement. Background technique [0002] Strain screening plays a pivotal role in biomedical experiments and industrial production. It is widely used in food health care, medicine, chemical industry and agriculture. Screen microbial strains that efficiently degrade lignocellulose from samples with high cellulose content such as straw, towels, and soil; screen microbial strains that efficiently degrade PVA from environments directly polluted by polyvinyl alcohol. [0003] Existing strain screening methods include: colony in situ hybridization screening method, immunoenzyme technology, chemiluminescent immunoassay and radioimmunoassay. These methods are all established on the basis of molecular biology or immunology. Usually, there are many screening procedures and a long period of time. Experienced technicians...

AI Technical Summary

Problems solved by technology

These methods are all established on the basis of molecular biology or immunology. Usually, there are many screening procedures and a long period of time. Experienced technicians are required to make judgments, which consume a lot of manpower and material resources.

Moreover, there is currently no device and method that can automatically determine the growth status of cloned strains and quickly screen out successfully cultured strains

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