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36results about How to "Screening results are reliable" patented technology

Method for screening feature peptide fragment for quantitatively detecting protein in food

ActiveCN107102149AMeet comprehensive screeningMeet the quantitative testing requirementsBiological testingMulti methodScreening method
The invention discloses a method for screening a feature peptide fragment for quantitatively detecting protein in food, and belongs to the technical field of protein quantitative detection. The screening method comprises the following steps: (1) comparing and analyzing matching score and interference number corresponding to a to-be-detected protein theoretical enzymolysis fragment by utilizing BLAST; (2) analyzing a mass spectrum response value and fragment abundance of a target peptide fragment through high performance liquid chromatography-tandem mass spectrometry; (3) calculating the enzymolysis rate of the target peptide fragment when the enzymolysis is conducted for 1 to 3 hours by utilizing a fitted curve; (4) calculating the recovery rate and the precision degree of the target peptide fragment. The feature peptide fragment suitable for protein accurate quantification is comprehensively screened from the aspects of specificity, mass spectrum characteristic, enzymolysis characteristic, accuracy, stability and the like and by utilizing various methods in a combined way; the screened feature peptide fragment can meet the quantitative detection requirement of high performance liquid chromatography-tandem mass spectrometry on protein in food matrix, and realizes protein quantitative detection requirement with high sensitivity, high precision degree and excellent reproducibility.
Owner:杭州璞湃科技有限公司

Method for screening wheat approximate variety by virtue of SSR molecular marker

InactiveCN105132551AError allowedWill not increase the quantity significantlyMicrobiological testing/measurementElectrophoresisFluorescence
The invention discloses a method for screening a wheat approximate variety by virtue of an SSR molecular marker. The method comprises the following steps: firstly, extracting DNA of an experimental variety; then, respectively implementing PCR amplification on template DNA of the experimental variety by virtue of 21 pairs of basic core SSR fluorescently-labeled primers as shown in SEQ ID NO. 1-42 in a sequence list and 21 pairs of extended core SSR fluorescently-labeled primers as shown in SEQ ID NO. 43-84 in the sequence list; acquiring data of a PCR product through electrophoresis detection; and then, analyzing the acquired data through PowerMarker so as to obtain a genetic distance between varieties, and judging the variety that genetic distance is less than 0.2 as an approximate variety. The method can be used for improving test precision and reducing test cost.
Owner:CROP RES INST SHANDONG ACAD OF AGRI SCI

Oil gas wildcat screening method

The present invention discloses a method for screening an oil gas wildcat target. The method comprises the following steps: obtaining a drilling success probability of a to-be-screened wildcat target and recoverable resource amount; obtaining a development risk scale of the to-be-screened wildcat target based on the drilling success probability and the recoverable resource amount; determining a threshold of the development risk scale based on an exploration target; and screening according to the threshold of the development risk scale, so as to obtain a wildcat target. According to the wildcat target screening method disclosed by the present invention, optimization of wildcats with different regions, different fields and different resource types on a unified platform can be realized, and not only operability and practicality of the screening method can be significantly improved, but also human subjective influences during a screening process are maximally reduced, and more reliable bases are provided for determination of a wildcat deployment direction and sequence, exploration investment optimization and layout plan making in an oil company.
Owner:CHINA PETROLEUM & CHEM CORP +1

Screening method of late bolting character of radish

The method for screening radish late bloting character includes the stage of vernalization and photostage, and adopts the following steps: disinfecting the seed to be identified and soaking the seed with sterile water for accelerating germination, inoculating the seed in sprouting state into MS culture medium, placing it into refrigerator, making artificial iarovization in time periods, after low-temp. treatment placing it into tissue culture chamber to make growth culture of radish seedling. In the photostage transplanting the radish seedling into the prenursery tray with matrix and placing it into tissue culture chamber and observing the bolting condition of squaring period by using different materials and making different treatments to identify the bloting characters of different materials, so that it can accelerate radish late bloting breeding process.
Owner:SHANGHAI JIAO TONG UNIV

Screening method of biocontrol bacteria and dosage for tobacco black shank

The invention discloses a method for screening biocontrol bacteria and dosage thereof against tobacco black shank. The method comprises the following steps: a. cultivating sterile tobacco seedlings on filter paper in a petri dish; b. on the basis of step a , add bacterial culture solution with biocontrol potential in the petri dish; c, after the tobacco seedlings and the bacterial suspension with biocontrol potential are cultivated together for 24 hours, add the zoospore suspension of tobacco black shank bacteria to the petri dish d, on the basis of step c, give the tobacco seedlings normal growth conditions, detect the control effect of the bacteria with biocontrol potential added by observing the morbidity of the tobacco seedlings after 7 days, and screen out the biocontrol that can prevent and treat tobacco black shank bacteria. Compared with the traditional method, the present invention has many advantages. It is simple, fast, less workload, high efficiency, accurate and reliable screening result, and can screen bacteria with bio-control function from a large number of unknown bacteria in a short period of time.
Owner:GUIZHOU TOBACCO SCI INST

Lactic acid bacterium culture medium

The invention discloses a lactic acid bacterium culture medium which comprises the following components: 1) 10-12g / L of peptone, 2) 4-6g / L of yeast extract, 3) 6-22g / L of glucose, 4) 2-7g / L of acid-base buffering agent, 5) 0.15-0.4g / L of growth factor, 6) 0-10g / L of beef extract, 7) 0-15g / L of soluble starch, 0-3g / L of fructose and 9) 0-20g / L of agar, wherein the pH value of the culture medium is 5.8 to 6.9. By utilizing the culture medium, the lactic acid bacterium can grow well, and moreover the problem that nearly all of the conventional commercial MRS culture media have the inbibitional effect to helicobacter pylori is solved, so that the culture medium can be used for screening lactic acid bacteria having the inbibitional effect to the helicobacter pylori, and a real and reliable screening result can be obtained in the screening experiment; and meanwhile by utilizing the culture medium, the cost of the lactic acid bacterium culture medium is greatly lowered.
Owner:BRIGHT DAIRY & FOOD CO LTD

Decision-making method of intelligentization building projects of intelligent power distribution network

Disclosed is a decision-making method of intelligentization building projects of an intelligent power distribution network. The method comprises the steps that an evaluation index system of the intelligent power distribution network is firstly built, then intelligent power distribution network data of the current year are substituted into the evaluation index system for calculation to obtain evaluation data, intelligentization building projects, to be decided in the next year, of the intelligent power distribution network are graded according to the obtained evaluation data so that project scores of all the intelligentization building projects can be obtained, and finally the intelligentization building projects needing to be invested in are screened out of the intelligentization building projects to be decided in the next year through a multi-objective comprehensive optimization algorithm according to the project scores of all the intelligentization building projects and information of the intelligentization building projects to be decided in the next year. The intelligentization building projects of the intelligent power distribution network are decided scientifically, objectively and reasonably.
Owner:STATE GRID CORP OF CHINA +2

Design method for preferring tea aroma electronic nose sensor array through processing technology

The invention discloses a design method for preferring a tea aroma electronic nose sensor array through a processing technology. The design method comprises the steps that sensors sensitive to tea aroma components are selected according to the tea aroma components of the specific processing technology, then sensors insensitive to the tea aroma are removed according to the sensitive degrees of theselected sensors in a tea aroma environment, and thus a primarily selected sensor set is obtained; then, relative coefficients between the sensors and distinguishing performance values of the sensorsare calculated; and the two numeral values are integrated for judging, optimal sensor combinations are screened to constitute the array, and finally, tea aroma detection and verification are conducted. According to the design method, by comparing the relative coefficients and the distinguishing performance values between the sensors, the aroma-sensitive sensor set capable of distinguishing the teaquality and the tea type is preferred.
Owner:ZHEJIANG UNIV

Pile brace supporting structure screening method based on sacrifice yield rate

The invention discloses a pile brace supporting structure screening method based on the sacrifice yield rate. The pile brace supporting structure screening method comprises the following steps that 1,a feasible region of a foundation pit pile brace supporting structure is established; 2, a noise factor set is established; 3, FLAC<3D> software is utilized to simulate the foundation pit excavationprocess, and a system response result is obtained; 4, robustness measurement indexes of the foundation pit pile brace supporting structure are calculated; 5, the cost of the foundation pit pile bracesupporting structure is calculated; 6, a Pareto leading edge is drawn according to the cost and the robustness measurement indexes; and 7, a knee point of the Pareto leading edge is obtained by applying the sacrifice yield rate, that is, the optimal foundation pit pile brace supporting structure designing scheme is obtained. By screening points of the Pareto leading edge, the influences of the similar points on knee point selection are weakened, the knee point is multiply screened to avoid trapping in the dilemma of local optimum, dependence on shape and curve fitting of the Pareto leading edge is small during knee point screening is small, and the screening result is accurate and reliable.
Owner:SHIJIAZHUANG TIEDAO UNIV +1

Method for screening high-secretion type material from tobacco mutants

The invention discloses a method for screening a high-secretion type material from a tobacco mutant library. The method comprises the following steps of: step (1): seed treatment: step (2): preliminary screening of mutation groups: step (3): screening and identification of single plants; step (4): repeated identification (F2-generation identification); step (5): strain identification. The method disclosed by the invention has the beneficial effects that at the initial stage of identification, the density and the type of glandular hairs of a leaf surface are intuitively screened by utilizing the correlation of the density and the type of the glandular hairs and secreta of the leaf surface, and then on the basis, identification of the secreta of the leaf surface and the screening of the high-secretion type material are carried out, so that the screening result is accurate and reliable; at a seedling stage, 90% or more of mutant plants are eliminated, so that the planting of large-field materials is greatly reduced, and the cost for land and labor and the like is reduced; because of reduction of the planting materials, the identification quantity of the secreta at a mature stage is also reduced, so that the cost is greatly reduced, the screening time is shortened and the screening efficiency is greatly improved.
Owner:TOBACCO RES INST HENAN ACADEMY OF AGRI SCI

Active fluorescence detection method of anthropogenic silent information regulatory factor 6 (Sirtuin6)

The invention discloses an active fluorescence detection method of an anthropogenic silent information regulatory factor 6 (Sirtuin6). A polypeptide containing fluorophore and quenching group (FRET (fluorescence resonance energy transfer) effect) and long-chain fatty acylation lysine residue is used as a substrate, the fluorescence intensity is associated with the enzymatic activity of Sirtuin6, and the Sirtuin6 recognition site (long-chain fatty acylation lysine) of the substrate is located in the middle of the substrate by the FRET effect, thus, the substrate (polypeptide) is close to a natural substrate of Sirtuin6, and the combination is close; more fluorophores can be selected, and reliable screening result can be obtained. The method can be applied to screening of the Sirtuin6 regulator or is used for active detection of Sirtuin6 in a biological sample, has the characteristics of miniaturization, automation, reliability, rapidness and the like, is suitable for high-reflux application, and also can be used for development of the novel Sirtuin6 active detection kit and screening of the Sirtuin6 regulator.
Owner:GUIYANG MEDICAL UNIVERSITY

Method for appraising starch decomposition capability of moulds

ActiveCN103290096AScreening results are reliableEliminate the step of inverting the plateMicrobiological testing/measurementBiotechnologyAmylase
The invention relates to a microorganism detection technology and in particular relates to a method for appraising the starch decomposition capability of moulds. The method comprises the following steps of: putting a liquid culture medium into a test tube; inoculating the moulds for generating amylase to decompose a milk-white liquid culture medium; observing a transparent zone generated by supernatant liquid in the test tube; after generating the transparent zone, obtaining culture liquid with the transparent zone in the test tube, measuring the reducing sugar content, and determining the enzyme production capability of the moulds. Compared with the conventional flat-plate screening method, the method has the characteristics that firstly, the culture medium is the liquid culture medium, agar is not added, the step of reversing a flat plate is removed, and the method is convenient; secondly, the observation mode is different from the conventional observation mode, the formed transparent zone is observed, the method is simple and easy to implement, the observation effect is remarkable, and a strain screening result is reliable; thirdly, the culture liquid with the transparent zone can be taken out and the reducing sugar content is directly measured.
Owner:山西省食品工业研究所

Screening method of salt enduring species of tomato

A method for screening tomato salt-tolerant traits, using the rooting conditions of tomato buds, the proliferation of cotyledon callus, the growth potential of seedlings, the rooting conditions of side branch cuttings at the adult plant stage and the next generation's rooting conditions under sodium chloride NaCl stress. The germination potential of the seeds can be used to comprehensively judge the salt tolerance of tomato plants, and at the same time accelerate the process of tomato salt-tolerant breeding. The method of the invention is simple and easy to master, and the salt tolerance screening of the whole system is under controllable conditions, so that the screening results are more reliable; the limited space of the tissue culture room is used for preliminary screening of a large number of materials; , which can not only reduce the workload, but also achieve uninterrupted breeding materials; the salt-tolerant plants can save one ear for topping, which can greatly shorten the breeding time.
Owner:SHANGHAI JIAOTONG UNIV

Protein drug auxiliary screening method

The invention provides a protein drug auxiliary screening method. The protein drug auxiliary screening method comprises the steps that a therapeutic protein and a plurality of alternate auxiliaries ofthe therapeutic protein are provided, the dissociation energy value between the auxiliaries and the therapeutic protein and the possible binding site of the auxiliaries and the therapeutic protein are analyzed by adopting AutoDockTools software and AggreScan online software to screen the multiple alternate auxiliaries, the screening result has a good corresponding relation to the result of a conventional stability experiment, which proves that the screening result of the protein drug auxiliary screening method is stable and reliable, meanwhile the method is easy and convenient to operate, andconsumes less time, only 1-2 days, to screen auxiliaries each time.
Owner:MABSPACE BIOSCIENCES (SUZHOU) CO LTD

New screen method of levofosfomycin strain converted by d-phosphonomycin as substrate

The invention relates to the technical field of biotransformation / biocatalysis, and relates to a novel screening method for levo-fosfomycin biotransformation strains with dextro-fosfomycin as the substrate. Solid medium containing the dextro-fosfomycin is utilized to culture strains to be tested; oversensitive micrococcus luteus and super-tolerance colon bacillus are utilized to be as the bioassay indicator bacteria, an agar block is placed on a bioassay tray to screen out levo-fosfomycin ransformation strains, then the products are identified by the combined method of the thin layer chromatography and the biological developer method to obtain positive strains to undergo complete liquid medium transformation with the dextro-fosfomycin as the substrate, transformation liquid is concentrated to undergo the product identification by the combined method of the thin layer chromatography and the biological developer method, and finally levo-fosfomycin biotransformation dominant strains are screened out. The method has low requirements for equipment, simple operation and low cost, and is suitable for the batch screening of the biotransformation bacteria.
Owner:SHENYANG PHARMA UNIVERSITY

Device and method for identifying development degree of ovary of eriocheir sinensis

The invention relates to a device and method for identifying the development degree of an ovary of eriocheir sinensis. The device comprises an identification pool and a cover plate located at the top of the identification pool, the identification pool comprises a male crab area located at a center and a plurality of channels coaxially arranged from inside to outside, a screening plate is arranged between every two adjacent channels, each screening plate is provided with a one-way door and an air hole, the number of the air holes increases gradually from the periphery of the identification pool to the male crab area, the number of the one-way doors decreases gradually from the periphery of the identification pool to the male crab area, the height of the one-way doors decreases gradually from the periphery of the identification pool to the male crab area according to an arithmetic progression, and the width of the channels increases gradually from the periphery of the identification pool to the male crab area according to a Peiboci progression. According to the device and method, the defect that the gonad development degree cannot be accurately judged by manually identifying external characteristics is overcome, feasible equipment is provided for automatically screening the gonad development maturity, and reference is provided for judging the commercial value of the eriocheir sinensis with different gonad development degrees.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +1

Rapid screening method of zinc finger protein

The invention relates to a rapid screening method of zinc finger protein. According to the invention, potential zinc finger protein target sequences and corresponding zinc fingers in gene are predicted by using on-line zinc finger protein prediction software; and overlapped polymerase chain reaction is carried out, such that a zinc finger protein library is obtained. The zinc finger proteins are fused with a transcriptional activation, such that a chimeric transcription factor is constructed. A chimeric promoter composed of a zinc finger protein target sequence and a promoter core element and a downstream reporter gene form a zinc finger protein detection carrier. A carrier expressing the chimeric transcription factor and the zinc finger protein detection carrier are co-transfected into a mammalian cell. Binding capacities of the zinc finger protein and the target sequence is reflected by the expression intensity of the reporter gene.
Owner:SHAANXI NORMAL UNIV

A screening and identification method for high temperature resistant cotton

InactiveCN104521747BIntuitive activityControl vertical and horizontal growthPlant genotype modificationTemperature stressAgricultural science
A high-temperature resistant cotton screening and identification method is used for screening and identification of high-temperature resistant cotton germplasm resource materials and varieties. Cotton materials are planted in the field by nutrition bowl seedling transplantation or direct seeding; mepiquat chloride at a concentration of 80-100 mg / L is used for processing when a main stem leaf grows 10-12 true leaves; after processing, fixed plants' height during 18-20d is measured and main stem internode length is limited; and according to average growth rate of plant height and average limited main stem internode length, the materials to be measured are divided into two types, namely a high-temperature resistant type and a high-temperature sensitive type. According to the high-temperature resistant cotton screening and identification method, no greenhouse facilities are needed to provide high temperature stress. By the method, costs are saved. The method is simple to operate, has advantages of standard and visual evaluation and reliable result, can provide rapid identification in early stage, and has important application value in the aspect of high-temperature resistant cotton breeding and production.
Owner:HUNAN AGRICULTURAL UNIV

A screening method for high-yielding cotton varieties

The invention belongs to the field of agriculture, and particularly relates to a screening method for a high-yield cotton variety. The screening method includes the steps that seeds are detinted, 100 seeds growing full after being detinted are selected, cottonseed kernel and cottonseed hull peeling is conducted on the 100 detinted full seeds, and the high-yield cotton variety is selected according to the weight ratio of cottonseed hulls and cottonseed kernels. The screening method for the high-yield cotton variety has high practicability and operability, screening results are stable and reliable, the high-yield cotton variety can be screened out fast and accurately through the method, the screened cotton variety can provide a basis for a breeder to select a cotton parent to prepare combinations, the breeding cycle is shortened, breeding blindness is reduced, and therefore huge economic benefits are obtained.
Owner:INST OF COTTON RES CHINESE ACAD OF AGRI SCI

Screening method for pile-supported structures based on sacrifice rate of return

The invention discloses a method for screening pile support structures based on sacrifice rate of return, comprising the following steps: step 1, constructing the feasible area of ​​foundation pit pile support structures; step 2, constructing a set of noise factors; step 3, using FLAC 3D The software simulates the excavation process of the foundation pit to obtain the system response results; step 4, calculate the robustness measurement index of the foundation pit pile support structure; step 5, calculate the cost of the foundation pit pile support structure; step 6, Draw the Pareto front according to the cost and the robustness measurement index; Step 7, apply the sacrifice rate of return to obtain the knee point of the Pareto front, that is, the optimal foundation pit pile support structure design scheme; the present invention passes Pareto Screening of points on the front edge weakens the influence of similar points on the selection of knee points, and multiple screening of knee points avoids falling into a local optimal dilemma. The present invention fits the shape and curve of the Pareto front when knee points are screened The dependence is small, and the screening results are accurate and reliable.
Owner:SHIJIAZHUANG TIEDAO UNIV +1

Method for quickly screening and testing cold resistance of tomatoes

The invention discloses a method for quickly screening and testing the cold resistance of tomatoes, which comprises: firstly, culturing tomato seedlings growing for 3 to 5 weeks, and processing stem apex leaves at 3 to 5 DEG C for 1 to 3 hours and at 3 DEG C below zero to 5 DEG C below zero for 7 to 9 hours; secondly, restoring the stem apex leaves at 3 to 5 DEG C for 10 to 14 hours and placing the stem apex leaves in light at 20 to 22 DEG C for 14 to 18 hours; and finally, measuring the electrical conductivity of the cold-treated and restored leaves, placing the leaves in distilled water, performing vacuumization and volume fixing, shaking the leaves at 21 to 23 DEG C under a sealed condition for 1 to 3 hours, measuring an electrical conductivity C1, sealing the leaves, boiling the leaves for 10 to 20 minutes, cooking the leaves to room temperature, measuring an electrical conductivity C2 and calculating a relative conductivity according to a formula of C=(C1-C0) / (C2-C0)x100, whereinwhen the value domain of the relative conductivity C is less than or equal to 42 percent, the tomato variety is a cold-resistance variety. In the invention, the cold resistance screening of the wholesystem is performed under controllable conditions, the result is reliable, the method is simple, convenient and easy to master, the breeding period is shortened greatly and the method can be used fortest the cold resistance of an introduced variety at the same time.
Owner:HEFEI UNIV OF TECH

A cell-screening model for downregulators targeting fungal cell wall chitin synthase

The invention relates to a target fungus cytoderm chitin synthetase decreasing agent high-throughput screening model, belonging to the field of genetic engineering, the model consists of a UMcsh5 gene promoter sequence UMPRO, a recombinant plasmid Ppic9KG-UMPRO-LUC, monoclonal cell strain GS115-UMPRO-LUC for stable expression of the UMPRO promoter, a screening reagent and a luciferase reporter gene detection system. The screening model has the advantages of simple and convenient operation, low cost, high flexibility, wide applicable range, high screening efficiency and the like and has great application potentiality.
Owner:MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI

Combustion catalyst screening device and method based on nitrocotton stability

The invention discloses a screening device and method for a combustion catalyst based on nitrocotton stability. The device comprises a sample tank, an airtight needle, a drying oven and a gas chromatograph, wherein the sample tank is composed of a base, a separation net, a tank body and a cap. The test method comprises the following steps: respectively putting nitrocotton and a mixture of nitrocotton and a combustion catalyst into a sample tank, humidifying, heating at 90 DEG C for 3 hours, and cooling; extracting reaction gas from the sample tanks, and injecting into a chromatographic instrument to detect the content of NO2, wherein the combustion catalyst which is more suitable for a nitrocotton-based solid propellant in the aspect of ensuring the chemical stability of nitrocotton can be screened out through detection results. During screening, influence factors of air and moisture are increased, an actual charging storage environment is close, the influence of poor heat conduction of nitrocotton on a detection result is avoided, and a combustion catalyst with small influence on chemical stability of the nitrocotton can be screened out.
Owner:XIAN MODERN CHEM RES INST

A screening method for characteristic peptides for quantitative detection of protein in food

ActiveCN107102149BMeet comprehensive screeningMeet the quantitative testing requirementsBiological testingScreening methodMulti method
The invention discloses a method for screening a feature peptide fragment for quantitatively detecting protein in food, and belongs to the technical field of protein quantitative detection. The screening method comprises the following steps: (1) comparing and analyzing matching score and interference number corresponding to a to-be-detected protein theoretical enzymolysis fragment by utilizing BLAST; (2) analyzing a mass spectrum response value and fragment abundance of a target peptide fragment through high performance liquid chromatography-tandem mass spectrometry; (3) calculating the enzymolysis rate of the target peptide fragment when the enzymolysis is conducted for 1 to 3 hours by utilizing a fitted curve; (4) calculating the recovery rate and the precision degree of the target peptide fragment. The feature peptide fragment suitable for protein accurate quantification is comprehensively screened from the aspects of specificity, mass spectrum characteristic, enzymolysis characteristic, accuracy, stability and the like and by utilizing various methods in a combined way; the screened feature peptide fragment can meet the quantitative detection requirement of high performance liquid chromatography-tandem mass spectrometry on protein in food matrix, and realizes protein quantitative detection requirement with high sensitivity, high precision degree and excellent reproducibility.
Owner:杭州璞湃科技有限公司

A method for identification of salt tolerance of different wheat varieties during germination

The invention discloses a method for identifying the salt tolerance of different wheat varieties in germination stage, and belongs to the field of agricultural technology application. The identification method of the salt tolerance of different wheat varieties in the germination stage of the present invention is the measured germination rate, germination potential, root length, first leaf length, coleoptile length, bud dry weight, seedling Calculate the relative value and weighted average of fresh weight, root dry weight, and dry matter transfer rate, import the weighted average of each index into the comprehensive index calculation formula, and calculate the comprehensive salt tolerance index. The identification method of the salt tolerance of different wheat varieties in the germination period is systematic, easy to operate, wide in adaptability and comprehensive, and is more accurate than the salt tolerance membership function value method for the evaluation of the salt tolerance of wheat germination period, and the result is more accurate. reliable.
Owner:QINGDAO AGRI UNIV

A kind of primer, kit and method for screening rice germplasm with early-growing and fast-growing tillers

The invention discloses a primer, a kit and a method for screening tillering, early-growing and fast-growing rice germplasm, and relates to the technical fields of molecular markers and crop genetics and breeding. It includes four sets of primers, and each set of primers includes a forward primer and a reverse primer. To the primers, the sequences of the four sets of primers are shown in SEQ ID NO: 1-8; the kit includes the above primers, PCR reaction buffer and Taq DNA polymerase; the screening method includes: extracting the total genomic DNA of the rice germplasm to be detected, Using it as a template, use the above primers or kits for PCR amplification, electrophoresis the obtained PCR amplification products, take pictures and analyze them in an agarose gel imaging system. The beneficial effect of the present invention is that the screening of tillering, early-growing and fast-growing rice germplasm can be realized through four sets of primers, which is accurate and free from external interference, and the detection of four molecular markers can be completed through one PCR amplification using the kit, It has the advantages of simplicity and efficiency.
Owner:JIANGXI AGRICULTURAL UNIVERSITY

Design scheme for enhancing anti-theft door modern performance

A design solution for improving modern performance of a theft-proof door belongs to mechanical and electric integration technical field. The inventor reuses own three items of inventive result and improves to form a reliable solution taking a telephone as the mechanical key to open the door, which scientifically solves various contradiction. The invention takes a seated phone, mobile phone namely general communicator as a receiver of the door opening signal, and is composed of a sampling circuit, an amplifying circuit, memory circuit, an eliminating resetting circuit, a control circuit and an automatic manual double-use lock, etc. The main principle is to take out the calling door opening signal from the receiver, processed by the circuits to form a door opening signal. The advantages is as follows, opening door is so modern without key that the invention can be operated by remote control in long distance, which is modern, with new trend and good accidental resistance. The most prominence is to ensure the integrity of the communication parts in the sampling process, so the invention has high comprehensive use value.
Owner:宁永敭

Micellar electrokinetic chromatography method for screening prolyl hydroxylase 2 inhibitor from traditional Chinese medicine extract

The invention discloses a method for accurately screening a prolyl hydroxylase 2 inhibitor in a traditional Chinese medicine extract by micellar electrokinetic chromatography, which comprises the following steps: sequentially injecting a prolyl hydroxylase 2 solution, a to-be-screened traditional Chinese medicine extract solution and an enzymatic reaction buffer solution into a centrifugal tube, then putting the centrifugal tube into a water bath at 20-45 DEG C to react for 0.5-5 hours, taking out the centrifugal tube, adding a quencher to terminate the reaction, and filtering to obtain the prolyl hydroxylase 2 inhibitor. Carrying out pressure sample introduction on a sample in a capillary electrophoresis apparatus, selecting a background buffer solution, applying a voltage of 10-30 kilovolts, carrying out separation detection, and representing enzyme activity by using a peak area of a product at 210 nm; whether the traditional Chinese medicine extract to be screened has the prolyl hydroxylase 2 inhibitory activity or not can be judged according to the change of the peak area of the product. The method is verified by a model inhibitor and investigated by methodology, the screening result is accurate and reliable, and the consumption of samples and reagents is small. Screening objects comprise mixtures, so that the application range is wider, and the method has important significance on development of traditional Chinese medicines and treatment of hypoxia-related diseases.
Owner:LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI

A screening device and identification method for soybean salt tolerance and salt-alkali tolerance

The invention discloses a soybean salt tolerance and saline-alkaline tolerance screening device and an identification method. The soybean salt tolerance and saline-alkaline tolerance screening device comprises a working frame and a solution stirring bucket; the working frame comprises an upper layer and a lower layer; the upper layer of the working frame is provided with a pass box and the lower layer is provided with a water supply tower which is used for supplying water or salt solution for the pass box; the solution stirring bucket and the water supply tower are connected through a water pipe; the water supply tower and the pass box are connected through a water pipe; a fixing net is fixedly arranged inside the pass box; a breeding pipe is arranged inside grids; the lateral wall surface and the bottom surface of the breeding pipe are provided with permeable holes; the solution permeates the breeding pipe through the permeable holes; water (or the salt solution) is automatically replaced through an electromagnet valve which is controlled by a timer. The soybean salt tolerance and saline-alkaline tolerance identification method established by utilizing the device is stable, scientific and simple and beneficial to large-scale identification and large-scale screening can be performed inside space with limited greenhouse, the degree of automation is high, the environmental influences are small, the repeatability is strong, and the screening result is reliable.
Owner:NANJING AGRICULTURAL UNIVERSITY
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