36results about How to "Screening results are reliable" patented technology

The invention discloses a method for screening a feature peptide fragment for quantitatively detecting protein in food, and belongs to the technical field of protein quantitative detection. The screening method comprises the following steps: (1) comparing and analyzing matching score and interference number corresponding to a to-be-detected protein theoretical enzymolysis fragment by utilizing BLAST; (2) analyzing a mass spectrum response value and fragment abundance of a target peptide fragment through high performance liquid chromatography-tandem mass spectrometry; (3) calculating the enzymolysis rate of the target peptide fragment when the enzymolysis is conducted for 1 to 3 hours by utilizing a fitted curve; (4) calculating the recovery rate and the precision degree of the target peptide fragment. The feature peptide fragment suitable for protein accurate quantification is comprehensively screened from the aspects of specificity, mass spectrum characteristic, enzymolysis characteristic, accuracy, stability and the like and by utilizing various methods in a combined way; the screened feature peptide fragment can meet the quantitative detection requirement of high performance liquid chromatography-tandem mass spectrometry on protein in food matrix, and realizes protein quantitative detection requirement with high sensitivity, high precision degree and excellent reproducibility.

The method for screening radish late bloting character includes the stage of vernalization and photostage, and adopts the following steps: disinfecting the seed to be identified and soaking the seed with sterile water for accelerating germination, inoculating the seed in sprouting state into MS culture medium, placing it into refrigerator, making artificial iarovization in time periods, after low-temp. treatment placing it into tissue culture chamber to make growth culture of radish seedling. In the photostage transplanting the radish seedling into the prenursery tray with matrix and placing it into tissue culture chamber and observing the bolting condition of squaring period by using different materials and making different treatments to identify the bloting characters of different materials, so that it can accelerate radish late bloting breeding process.

The invention discloses a pile brace supporting structure screening method based on the sacrifice yield rate. The pile brace supporting structure screening method comprises the following steps that 1,a feasible region of a foundation pit pile brace supporting structure is established; 2, a noise factor set is established; 3, FLAC<3D> software is utilized to simulate the foundation pit excavationprocess, and a system response result is obtained; 4, robustness measurement indexes of the foundation pit pile brace supporting structure are calculated; 5, the cost of the foundation pit pile bracesupporting structure is calculated; 6, a Pareto leading edge is drawn according to the cost and the robustness measurement indexes; and 7, a knee point of the Pareto leading edge is obtained by applying the sacrifice yield rate, that is, the optimal foundation pit pile brace supporting structure designing scheme is obtained. By screening points of the Pareto leading edge, the influences of the similar points on knee point selection are weakened, the knee point is multiply screened to avoid trapping in the dilemma of local optimum, dependence on shape and curve fitting of the Pareto leading edge is small during knee point screening is small, and the screening result is accurate and reliable.

The invention provides a protein drug auxiliary screening method. The protein drug auxiliary screening method comprises the steps that a therapeutic protein and a plurality of alternate auxiliaries ofthe therapeutic protein are provided, the dissociation energy value between the auxiliaries and the therapeutic protein and the possible binding site of the auxiliaries and the therapeutic protein are analyzed by adopting AutoDockTools software and AggreScan online software to screen the multiple alternate auxiliaries, the screening result has a good corresponding relation to the result of a conventional stability experiment, which proves that the screening result of the protein drug auxiliary screening method is stable and reliable, meanwhile the method is easy and convenient to operate, andconsumes less time, only 1-2 days, to screen auxiliaries each time.

The invention relates to a device and method for identifying the development degree of an ovary of eriocheir sinensis. The device comprises an identification pool and a cover plate located at the top of the identification pool, the identification pool comprises a male crab area located at a center and a plurality of channels coaxially arranged from inside to outside, a screening plate is arranged between every two adjacent channels, each screening plate is provided with a one-way door and an air hole, the number of the air holes increases gradually from the periphery of the identification pool to the male crab area, the number of the one-way doors decreases gradually from the periphery of the identification pool to the male crab area, the height of the one-way doors decreases gradually from the periphery of the identification pool to the male crab area according to an arithmetic progression, and the width of the channels increases gradually from the periphery of the identification pool to the male crab area according to a Peiboci progression. According to the device and method, the defect that the gonad development degree cannot be accurately judged by manually identifying external characteristics is overcome, feasible equipment is provided for automatically screening the gonad development maturity, and reference is provided for judging the commercial value of the eriocheir sinensis with different gonad development degrees.

The invention discloses a screening device and method for a combustion catalyst based on nitrocotton stability. The device comprises a sample tank, an airtight needle, a drying oven and a gas chromatograph, wherein the sample tank is composed of a base, a separation net, a tank body and a cap. The test method comprises the following steps: respectively putting nitrocotton and a mixture of nitrocotton and a combustion catalyst into a sample tank, humidifying, heating at 90 DEG C for 3 hours, and cooling; extracting reaction gas from the sample tanks, and injecting into a chromatographic instrument to detect the content of NO2, wherein the combustion catalyst which is more suitable for a nitrocotton-based solid propellant in the aspect of ensuring the chemical stability of nitrocotton can be screened out through detection results. During screening, influence factors of air and moisture are increased, an actual charging storage environment is close, the influence of poor heat conduction of nitrocotton on a detection result is avoided, and a combustion catalyst with small influence on chemical stability of the nitrocotton can be screened out.

A design solution for improving modern performance of a theft-proof door belongs to mechanical and electric integration technical field. The inventor reuses own three items of inventive result and improves to form a reliable solution taking a telephone as the mechanical key to open the door, which scientifically solves various contradiction. The invention takes a seated phone, mobile phone namely general communicator as a receiver of the door opening signal, and is composed of a sampling circuit, an amplifying circuit, memory circuit, an eliminating resetting circuit, a control circuit and an automatic manual double-use lock, etc. The main principle is to take out the calling door opening signal from the receiver, processed by the circuits to form a door opening signal. The advantages is as follows, opening door is so modern without key that the invention can be operated by remote control in long distance, which is modern, with new trend and good accidental resistance. The most prominence is to ensure the integrity of the communication parts in the sampling process, so the invention has high comprehensive use value.

The invention discloses a method for accurately screening a prolyl hydroxylase 2 inhibitor in a traditional Chinese medicine extract by micellar electrokinetic chromatography, which comprises the following steps: sequentially injecting a prolyl hydroxylase 2 solution, a to-be-screened traditional Chinese medicine extract solution and an enzymatic reaction buffer solution into a centrifugal tube, then putting the centrifugal tube into a water bath at 20-45 DEG C to react for 0.5-5 hours, taking out the centrifugal tube, adding a quencher to terminate the reaction, and filtering to obtain the prolyl hydroxylase 2 inhibitor. Carrying out pressure sample introduction on a sample in a capillary electrophoresis apparatus, selecting a background buffer solution, applying a voltage of 10-30 kilovolts, carrying out separation detection, and representing enzyme activity by using a peak area of a product at 210 nm; whether the traditional Chinese medicine extract to be screened has the prolyl hydroxylase 2 inhibitory activity or not can be judged according to the change of the peak area of the product. The method is verified by a model inhibitor and investigated by methodology, the screening result is accurate and reliable, and the consumption of samples and reagents is small. Screening objects comprise mixtures, so that the application range is wider, and the method has important significance on development of traditional Chinese medicines and treatment of hypoxia-related diseases.