36results about How to "Screening results are reliable" patented technology

A method for screening tomato salt-tolerant traits, using the rooting conditions of tomato buds, the proliferation of cotyledon callus, the growth potential of seedlings, the rooting conditions of side branch cuttings at the adult plant stage and the next generation's rooting conditions under sodium chloride NaCl stress. The germination potential of the seeds can be used to comprehensively judge the salt tolerance of tomato plants, and at the same time accelerate the process of tomato salt-tolerant breeding. The method of the invention is simple and easy to master, and the salt tolerance screening of the whole system is under controllable conditions, so that the screening results are more reliable; the limited space of the tissue culture room is used for preliminary screening of a large number of materials; , which can not only reduce the workload, but also achieve uninterrupted breeding materials; the salt-tolerant plants can save one ear for topping, which can greatly shorten the breeding time.

The invention relates to a rapid screening method of zinc finger protein. According to the invention, potential zinc finger protein target sequences and corresponding zinc fingers in gene are predicted by using on-line zinc finger protein prediction software; and overlapped polymerase chain reaction is carried out, such that a zinc finger protein library is obtained. The zinc finger proteins are fused with a transcriptional activation, such that a chimeric transcription factor is constructed. A chimeric promoter composed of a zinc finger protein target sequence and a promoter core element and a downstream reporter gene form a zinc finger protein detection carrier. A carrier expressing the chimeric transcription factor and the zinc finger protein detection carrier are co-transfected into a mammalian cell. Binding capacities of the zinc finger protein and the target sequence is reflected by the expression intensity of the reporter gene.

The invention discloses a method for screening pile support structures based on sacrifice rate of return, comprising the following steps: step 1, constructing the feasible area of ​​foundation pit pile support structures; step 2, constructing a set of noise factors; step 3, using FLAC 3D The software simulates the excavation process of the foundation pit to obtain the system response results; step 4, calculate the robustness measurement index of the foundation pit pile support structure; step 5, calculate the cost of the foundation pit pile support structure; step 6, Draw the Pareto front according to the cost and the robustness measurement index; Step 7, apply the sacrifice rate of return to obtain the knee point of the Pareto front, that is, the optimal foundation pit pile support structure design scheme; the present invention passes Pareto Screening of points on the front edge weakens the influence of similar points on the selection of knee points, and multiple screening of knee points avoids falling into a local optimal dilemma. The present invention fits the shape and curve of the Pareto front when knee points are screened The dependence is small, and the screening results are accurate and reliable.

The invention discloses a method for quickly screening and testing the cold resistance of tomatoes, which comprises: firstly, culturing tomato seedlings growing for 3 to 5 weeks, and processing stem apex leaves at 3 to 5 DEG C for 1 to 3 hours and at 3 DEG C below zero to 5 DEG C below zero for 7 to 9 hours; secondly, restoring the stem apex leaves at 3 to 5 DEG C for 10 to 14 hours and placing the stem apex leaves in light at 20 to 22 DEG C for 14 to 18 hours; and finally, measuring the electrical conductivity of the cold-treated and restored leaves, placing the leaves in distilled water, performing vacuumization and volume fixing, shaking the leaves at 21 to 23 DEG C under a sealed condition for 1 to 3 hours, measuring an electrical conductivity C1, sealing the leaves, boiling the leaves for 10 to 20 minutes, cooking the leaves to room temperature, measuring an electrical conductivity C2 and calculating a relative conductivity according to a formula of C=(C1-C0) / (C2-C0)x100, whereinwhen the value domain of the relative conductivity C is less than or equal to 42 percent, the tomato variety is a cold-resistance variety. In the invention, the cold resistance screening of the wholesystem is performed under controllable conditions, the result is reliable, the method is simple, convenient and easy to master, the breeding period is shortened greatly and the method can be used fortest the cold resistance of an introduced variety at the same time.

The invention relates to a target fungus cytoderm chitin synthetase decreasing agent high-throughput screening model, belonging to the field of genetic engineering, the model consists of a UMcsh5 gene promoter sequence UMPRO, a recombinant plasmid Ppic9KG-UMPRO-LUC, monoclonal cell strain GS115-UMPRO-LUC for stable expression of the UMPRO promoter, a screening reagent and a luciferase reporter gene detection system. The screening model has the advantages of simple and convenient operation, low cost, high flexibility, wide applicable range, high screening efficiency and the like and has great application potentiality.

The method for screening radish late bloting character includes the stage of vernalization and photostage, and adopts the following steps: disinfecting the seed to be identified and soaking the seed with sterile water for accelerating germination, inoculating the seed in sprouting state into MS culture medium, placing it into refrigerator, making artificial iarovization in time periods, after low-temp. treatment placing it into tissue culture chamber to make growth culture of radish seedling. In the photostage transplanting the radish seedling into the prenursery tray with matrix and placing it into tissue culture chamber and observing the bolting condition of squaring period by using different materials and making different treatments to identify the bloting characters of different materials, so that it can accelerate radish late bloting breeding process.

The invention discloses a method for screening a feature peptide fragment for quantitatively detecting protein in food, and belongs to the technical field of protein quantitative detection. The screening method comprises the following steps: (1) comparing and analyzing matching score and interference number corresponding to a to-be-detected protein theoretical enzymolysis fragment by utilizing BLAST; (2) analyzing a mass spectrum response value and fragment abundance of a target peptide fragment through high performance liquid chromatography-tandem mass spectrometry; (3) calculating the enzymolysis rate of the target peptide fragment when the enzymolysis is conducted for 1 to 3 hours by utilizing a fitted curve; (4) calculating the recovery rate and the precision degree of the target peptide fragment. The feature peptide fragment suitable for protein accurate quantification is comprehensively screened from the aspects of specificity, mass spectrum characteristic, enzymolysis characteristic, accuracy, stability and the like and by utilizing various methods in a combined way; the screened feature peptide fragment can meet the quantitative detection requirement of high performance liquid chromatography-tandem mass spectrometry on protein in food matrix, and realizes protein quantitative detection requirement with high sensitivity, high precision degree and excellent reproducibility.

The invention discloses a soybean salt tolerance and saline-alkaline tolerance screening device and an identification method. The soybean salt tolerance and saline-alkaline tolerance screening device comprises a working frame and a solution stirring bucket; the working frame comprises an upper layer and a lower layer; the upper layer of the working frame is provided with a pass box and the lower layer is provided with a water supply tower which is used for supplying water or salt solution for the pass box; the solution stirring bucket and the water supply tower are connected through a water pipe; the water supply tower and the pass box are connected through a water pipe; a fixing net is fixedly arranged inside the pass box; a breeding pipe is arranged inside grids; the lateral wall surface and the bottom surface of the breeding pipe are provided with permeable holes; the solution permeates the breeding pipe through the permeable holes; water (or the salt solution) is automatically replaced through an electromagnet valve which is controlled by a timer. The soybean salt tolerance and saline-alkaline tolerance identification method established by utilizing the device is stable, scientific and simple and beneficial to large-scale identification and large-scale screening can be performed inside space with limited greenhouse, the degree of automation is high, the environmental influences are small, the repeatability is strong, and the screening result is reliable.