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A screening and identification method for high temperature resistant cotton

An identification method and high-temperature-resistant technology, applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve the problem of being susceptible to natural temperature changes and other conditions, the pollen germination test is not rigorous enough, and the high temperature treatment is not easy to control. and other problems, to achieve the effects of accurate and reliable screening results, weakening of terminal bud growth, and intuitive evaluation criteria

Inactive Publication Date: 2016-08-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method needs to build a simple high-temperature shed for artificial high-temperature stress treatment at the initial flowering stage of cotton. The high-temperature treatment is not easy to control and is easily affected by changes in natural environment temperature and other conditions. Moreover, the pollen germination test is not rigorous enough, and the judgment standard is vague.

Method used

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  • A screening and identification method for high temperature resistant cotton
  • A screening and identification method for high temperature resistant cotton
  • A screening and identification method for high temperature resistant cotton

Examples

Experimental program
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Effect test

Embodiment 1

[0025] In 2012, the invention was used to screen and identify the high temperature tolerance of 14 cotton materials (see Table 1 for material names) at the Yunyuan Experimental Base of Hunan Agricultural University. Adopt the method of transplanting seedlings in nutrient pots, sow on April 21 and transplant on May 18, every 667m 23,000 plants were planted, 100 plants were planted for each material, and managed according to conventional planting methods after transplanting, without any chemical regulation at the seedling stage. On June 24th, the 30Kg solution of 80mg / L metrendine (merepepter is 97% powder produced by Xiaokang Pesticide Co., Ltd., Anyang City, Henan Province, prepared by dissolving with tap water according to the instructions) with a sprayer at 9:00 am will be prepared on June 24. From 00 onwards, each material to be tested was evenly sprayed, and the whole plant was sprayed. At this time, each material has entered the full bud stage, and the main stem has abou...

Embodiment 2

[0032] In order to verify the accuracy and application value of the present invention, 14 parts of cotton materials in Example 1 were continued to be planted in Guanshan Village, Ningxiang County, Changsha City, Hunan Province in 2013. Adopt the method of transplanting seedlings in a nutritional pot, sow on April 13, transplant on May 13, every 667m 2 2500 plants were planted, 100 plants were planted for each material, and managed according to conventional planting methods after transplanting. Around June 20, all the materials entered the blooming period. From June 13 to 25, it encountered a period of high temperature weather exceeding 35°C, and then from June 30 to August 19, there was a rare high temperature and dry weather for more than 50 consecutive days. With the help of high temperature stress that occurs naturally in the field, we confirmed the high temperature tolerance of the cotton materials screened and identified in Example 1 using the present invention. During ...

Embodiment 3

[0036] In 2014, in Longwangmiao Village, Chunhua Town, Changsha County, Hunan Province, the invention was used to screen and identify the high temperature tolerance of 10 cotton varieties (see Table 3 for material names). Seeds were broadcasted live on May 12, 100 holes were broadcasted for each material to be identified, 3 to 4 seeds were sown in each hole, 1 to 2 seedlings were reserved in each hole at the stage of 1 true leaf, and 1 seedling was settled in each hole at the stage of 3 true leaves . The planting density is every 667m 2 Plant 2,000 plants, ensure sufficient fertilizer and water supply and normal pest management according to conventional methods, without any chemical regulation at the seedling stage. On July 6th, the 30Kg solution that will prepare the concentration that is 100mg / L metrendine (the merepeptide source and preparation method are the same as embodiment 1) sprays each material to be tested evenly after 15:00 in the afternoon with a sprayer, and the...

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Abstract

A high-temperature resistant cotton screening and identification method is used for screening and identification of high-temperature resistant cotton germplasm resource materials and varieties. Cotton materials are planted in the field by nutrition bowl seedling transplantation or direct seeding; mepiquat chloride at a concentration of 80-100 mg / L is used for processing when a main stem leaf grows 10-12 true leaves; after processing, fixed plants' height during 18-20d is measured and main stem internode length is limited; and according to average growth rate of plant height and average limited main stem internode length, the materials to be measured are divided into two types, namely a high-temperature resistant type and a high-temperature sensitive type. According to the high-temperature resistant cotton screening and identification method, no greenhouse facilities are needed to provide high temperature stress. By the method, costs are saved. The method is simple to operate, has advantages of standard and visual evaluation and reliable result, can provide rapid identification in early stage, and has important application value in the aspect of high-temperature resistant cotton breeding and production.

Description

technical field [0001] The invention relates to a method for screening and identification of high-temperature-resistant cotton, which is specially used for screening and identification of high-temperature-resistant cotton germplasm resource materials and varieties, and belongs to the field of stress-resistant breeding of crops. Background technique [0002] Global warming has become a consensus. It is predicted that by the second half of this century, the average global temperature will rise by 1.4-5.8°C, and extreme climates such as high temperature and drought will occur more frequently. The average annual temperature increase in my country is slightly higher than global warming by 0.5-0.8°C, and the probability of extreme high temperatures in the future summer (June to August) is as high as 90% (Battisti D Sand Naylor R L. Historical Warnings of Future Food Insecurity with Unprecedented Seasonal Heat. Science, 2009, 323:240-244). At present, seasonal high temperatures ex...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/04
Inventor 刘志袁小玲陈鹏云潘家峰
Owner HUNAN AGRICULTURAL UNIV
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