A screening and identification method for high temperature resistant cotton
An identification method and high-temperature-resistant technology, applied in the fields of botanical equipment and methods, applications, and plant genetic improvement, can solve the problem of being susceptible to natural temperature changes and other conditions, the pollen germination test is not rigorous enough, and the high temperature treatment is not easy to control. and other problems, to achieve the effects of accurate and reliable screening results, weakening of terminal bud growth, and intuitive evaluation criteria
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Embodiment 1
[0025] In 2012, the invention was used to screen and identify the high temperature tolerance of 14 cotton materials (see Table 1 for material names) at the Yunyuan Experimental Base of Hunan Agricultural University. Adopt the method of transplanting seedlings in nutrient pots, sow on April 21 and transplant on May 18, every 667m 23,000 plants were planted, 100 plants were planted for each material, and managed according to conventional planting methods after transplanting, without any chemical regulation at the seedling stage. On June 24th, the 30Kg solution of 80mg / L metrendine (merepepter is 97% powder produced by Xiaokang Pesticide Co., Ltd., Anyang City, Henan Province, prepared by dissolving with tap water according to the instructions) with a sprayer at 9:00 am will be prepared on June 24. From 00 onwards, each material to be tested was evenly sprayed, and the whole plant was sprayed. At this time, each material has entered the full bud stage, and the main stem has abou...
Embodiment 2
[0032] In order to verify the accuracy and application value of the present invention, 14 parts of cotton materials in Example 1 were continued to be planted in Guanshan Village, Ningxiang County, Changsha City, Hunan Province in 2013. Adopt the method of transplanting seedlings in a nutritional pot, sow on April 13, transplant on May 13, every 667m 2 2500 plants were planted, 100 plants were planted for each material, and managed according to conventional planting methods after transplanting. Around June 20, all the materials entered the blooming period. From June 13 to 25, it encountered a period of high temperature weather exceeding 35°C, and then from June 30 to August 19, there was a rare high temperature and dry weather for more than 50 consecutive days. With the help of high temperature stress that occurs naturally in the field, we confirmed the high temperature tolerance of the cotton materials screened and identified in Example 1 using the present invention. During ...
Embodiment 3
[0036] In 2014, in Longwangmiao Village, Chunhua Town, Changsha County, Hunan Province, the invention was used to screen and identify the high temperature tolerance of 10 cotton varieties (see Table 3 for material names). Seeds were broadcasted live on May 12, 100 holes were broadcasted for each material to be identified, 3 to 4 seeds were sown in each hole, 1 to 2 seedlings were reserved in each hole at the stage of 1 true leaf, and 1 seedling was settled in each hole at the stage of 3 true leaves . The planting density is every 667m 2 Plant 2,000 plants, ensure sufficient fertilizer and water supply and normal pest management according to conventional methods, without any chemical regulation at the seedling stage. On July 6th, the 30Kg solution that will prepare the concentration that is 100mg / L metrendine (the merepeptide source and preparation method are the same as embodiment 1) sprays each material to be tested evenly after 15:00 in the afternoon with a sprayer, and the...
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