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Screening method of late bolting character of radish

A screening method and technology of radish, which is applied in the field of screening of late bolting traits of radish, can solve problems such as limiting radish breeding process, achieve reliable screening results, save land resources, and speed up identification

Inactive Publication Date: 2005-11-23
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, radish can only bloom once a year under natural conditions, which greatly limits the radish breeding process (Chen Huoying, etc., CPPU on the influence of radish bran heart, bolting and yield in spring greenhouses, Journal of Shanghai Agricultural University, 1999, 17 (1 ), 48-51), (Wang Longzhi, Chen Huoying, etc., Four Seasons Cultivation of Radish with High Quality and High Efficiency, Science and Technology Literature Publishing House, 2001)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 75% each from 8 materials of Bai Yuchun (from South Korea), Tianchun Dagen and Siji Xiaozheng (from Japan) and domestic existing varieties Wuhan Chunbulao, Shangnong 936, Duanye 13, Liushiri and Mid-Autumn Red. Seeds (divided into 5 parts, that is, 5 treatments, 15 seeds per treatment), sterilized with saturated bleach solution for 20 minutes, then soaked in sterile water at 25±1°C for 24 hours under sterile conditions, and germinated for 2 hours. Afterwards, inoculate the germinated seeds (radicle about 1-2 mm in length) in MS macronutrient medium, and then place 4 treatment cultures of each of the 8 materials in a refrigerator with temperature controlled at 3±1°C Carry out low-temperature treatment, and after 5 days, 10 days, 15 days, and 20 days of low-temperature treatment, the cultures after low-temperature treatment are transferred to a tissue culture room with a temperature controlled at 25±1°C and 16 hours of light per day to grow radish seedlings. Growth c...

Embodiment 2

[0019] According to the present invention, the Korean variety Bai Yuchun, which has late bolting characteristics and excellent comprehensive agronomic traits, is used as one of the targets for the localization of spring radish varieties, and is subjected to self-separation at the bud stage. And according to the results of the present invention, the temperature of the low temperature treatment is set to 3±1° C., and the treatment time is set to 30 days and 40 days, so as to ensure that the seeds are received during artificial vernalization. Specific implementation plan:

[0020] On April 18 and April 28, 1998, 10 seeds of Bai Yuchun were sterilized with saturated bleach solution for 20 minutes, and then soaked in sterile water at 25±1°C for 24 hours under sterile conditions to accelerate germination. 2 days; on April 21 and May 1, inoculate the germinated seeds (radicle about 1 mm in length) in MS macronutrient medium, and then place the culture in a refrigerator with a tempera...

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Abstract

The method for screening radish late bloting character includes the stage of vernalization and photostage, and adopts the following steps: disinfecting the seed to be identified and soaking the seed with sterile water for accelerating germination, inoculating the seed in sprouting state into MS culture medium, placing it into refrigerator, making artificial iarovization in time periods, after low-temp. treatment placing it into tissue culture chamber to make growth culture of radish seedling. In the photostage transplanting the radish seedling into the prenursery tray with matrix and placing it into tissue culture chamber and observing the bolting condition of squaring period by using different materials and making different treatments to identify the bloting characters of different materials, so that it can accelerate radish late bloting breeding process.

Description

Technical field: [0001] The invention relates to a screening method for late bolting traits of radishes, which is used for the introduction and breeding of biennial vegetables such as radishes in spring and winter-spring special varieties, and belongs to the technical field of crop breeding. Background technique: [0002] Radish (Raphanus sativus L.) is one of the main vegetables widely cultivated in Southeast Asia. It is not only rich in nutrition, but also has high medicinal value. Although our country has a long history of cultivating radishes and abundant variety resources, there is still a big gap between the research and utilization of variety resources compared with the advanced level in foreign countries. [0003] At present, there are few radish varieties suitable for off-season cultivation, and the low quality is one of the main problems in radish production. Radish is a biennial vegetable crop, and the normal cultivation season is autumn and autumn and winter. T...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00A01H3/02A01H4/00
Inventor 陈火英张建华朱丽萍
Owner SHANGHAI JIAOTONG UNIV
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