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Method for appraising starch decomposition capability of moulds

A technology of mold and ability, applied in the field of microbial detection, to achieve the effect of convenient method, obvious observation effect, and reliable results of strain screening

Active Publication Date: 2013-09-11
山西省食品工业研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method generally does not involve the identification of the starch-producing ability of the strain

Method used

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  • Method for appraising starch decomposition capability of moulds

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Experimental program
Comparison scheme
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Embodiment 1

[0008] Embodiment 1, a kind of method for identifying mold decomposing starch ability, the steps are as follows:

[0009] 1 Preparation of liquid culture medium

[0010] Weigh NaNO 3 0.2g~0.3g, K 2 HPO 4 0.1g, KCl 0.05g, MgSO 4 0.05g, FeSO 4 0.001g, soluble starch 1%~5%. Add 100ml of purified water, mix and heat to boil. Dispense into test tubes while hot, each test tube filled with 10ml.

[0011] 2 access mold

[0012] Inject mold No. 1 and No. 2 into two test tubes with an inoculation needle under sterile conditions.

[0013] Mold 1 Name: Aspergillus, Aspergillus niger, AS.3324

[0014] Mold 2 Name: Aspergillus, Aspergillus niger, AS.34309

[0015] 3 training

[0016] Place the test tube inserted into the mold in a 30°C incubator and cultivate it for 5-7 days.

[0017] 4 Observing preliminary qualitative results

[0018] According to whether the zona pellucida is produced in the two test tubes, the level of the enzyme-producing ability of the mold is judg...

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Abstract

The invention relates to a microorganism detection technology and in particular relates to a method for appraising the starch decomposition capability of moulds. The method comprises the following steps of: putting a liquid culture medium into a test tube; inoculating the moulds for generating amylase to decompose a milk-white liquid culture medium; observing a transparent zone generated by supernatant liquid in the test tube; after generating the transparent zone, obtaining culture liquid with the transparent zone in the test tube, measuring the reducing sugar content, and determining the enzyme production capability of the moulds. Compared with the conventional flat-plate screening method, the method has the characteristics that firstly, the culture medium is the liquid culture medium, agar is not added, the step of reversing a flat plate is removed, and the method is convenient; secondly, the observation mode is different from the conventional observation mode, the formed transparent zone is observed, the method is simple and easy to implement, the observation effect is remarkable, and a strain screening result is reliable; thirdly, the culture liquid with the transparent zone can be taken out and the reducing sugar content is directly measured.

Description

technical field [0001] The invention relates to a microorganism detection technology, in particular to a method for identifying the ability of mold to decompose starch. Background technique [0002] Molds are widely distributed in nature, and they are closely related to human life. They are a type of microorganisms that were recognized and used early in human life. Mold has a strong ability to decompose various organic substances and is widely used in the field of food processing. There are many types of molds, and many molds can produce amylase, which breaks down starch. The ability of different molds to decompose starch has a large gap. Usually, the method of identifying the type or performance of mold is the plate screening method. Generally, the solid medium is solidified in a petri dish to form a plate state, and then the mold is inoculated. According to the number of plaques or the size of the transparent circle formed on the edge of a single colony, the ratio is sim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/40C12Q1/04
Inventor 成剑峰郭文娟
Owner 山西省食品工业研究所
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