A kind of primer, kit and method for screening rice germplasm with early-growing and fast-growing tillers

Abstract

The invention discloses a primer, a kit and a method for screening tillering, early-growing and fast-growing rice germplasm, and relates to the technical fields of molecular markers and crop genetics and breeding. It includes four sets of primers, and each set of primers includes a forward primer and a reverse primer. To the primers, the sequences of the four sets of primers are shown in SEQ ID NO: 1-8; the kit includes the above primers, PCR reaction buffer and Taq DNA polymerase; the screening method includes: extracting the total genomic DNA of the rice germplasm to be detected, Using it as a template, use the above primers or kits for PCR amplification, electrophoresis the obtained PCR amplification products, take pictures and analyze them in an agarose gel imaging system. The beneficial effect of the present invention is that the screening of tillering, early-growing and fast-growing rice germplasm can be realized through four sets of primers, which is accurate and free from external interference, and the detection of four molecular markers can be completed through one PCR amplification using the kit, It has the advantages of simplicity and efficiency.

Description

technical field [0001] The invention relates to the technical field of molecular markers and the technical field of crop genetics and breeding, in particular to a primer, a kit and a method for screening tillering, early-growing and rapid-onset rice germplasm. Background technique [0002] Early rice growth and rapid growth refers to early tillering and fast tillering in the early stage of the rice population, and the theoretical effective number of tillers can be reached as early as possible, so as to improve the ear formation rate and the uniformity of rice ears, and finally achieve stable and high yield of rice. Therefore, screening rice germplasm with early and rapid tillering is of great significance for breeding stable and high-yielding rice varieties. [0003] The trait of rice tiller early growth and rapid onset is a quantitative trait regulated by multiple genes, but it is easily affected by factors such as rice planting density, fertilizer and water management, and...

AI Technical Summary

Problems solved by technology

First of all, in the process of screening rice germplasm for tillering, early growth and rapid emergence in the field, breeders are easily disturbed by the climate conditions, soil conditions, and cultivation management measures of the rice planting site, which may cause distortion of the trait identification results

Secondly, to observe or investigate the early-growing and rapid-onset traits of rice tillering requires fixed-point, continuous observation and investigation of rice tillering dynamics when rice enters the tillering stage, which requires a large workload and long duration, and consumes a lot of human resources.

Finally, due to the differences in regional climate conditions, soil conditions, cultivation management measures of different research units, and the different evaluation standards of different researchers, there is a lack of comparability between the identification results of different research units

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