Method for screening wheat approximate variety by virtue of SSR molecular marker

Abstract

The invention discloses a method for screening a wheat approximate variety by virtue of an SSR molecular marker. The method comprises the following steps: firstly, extracting DNA of an experimental variety; then, respectively implementing PCR amplification on template DNA of the experimental variety by virtue of 21 pairs of basic core SSR fluorescently-labeled primers as shown in SEQ ID NO. 1-42 in a sequence list and 21 pairs of extended core SSR fluorescently-labeled primers as shown in SEQ ID NO. 43-84 in the sequence list; acquiring data of a PCR product through electrophoresis detection; and then, analyzing the acquired data through PowerMarker so as to obtain a genetic distance between varieties, and judging the variety that genetic distance is less than 0.2 as an approximate variety. The method can be used for improving test precision and reducing test cost.

Description

technical field [0001] The invention relates to a method for screening similar varieties of wheat, which belongs to the technical field of wheat breeding and application in agriculture. Background technique [0002] Wheat belongs to the Triticum genus of the grass family, and it is one of the earliest cultivated crops in the world. In 1997, my country began to implement the new plant variety protection system. According to China's "Regulations on the Protection of New Varieties of Plants", new plant varieties must have specificity, consistency and stability (DUS for short) in order to grant variety rights. In recent years, with the increasing awareness of variety rights among Chinese plant breeders, the number of new plant varieties applying for protection has increased year by year. Not only that, the Ministry of Agriculture's "Major Crop Variety Approval Measures" requires that wheat varieties applying for approval must have specificity, consistency and stability. [00...

AI Technical Summary

Problems solved by technology

This method has the following disadvantages: (1) The number of known varieties of wheat is large, the variety types and grouping characters are few, and the similarity of bred varieties is high. Dozens or even hundreds of similar varieties are screened out from a large number of applied varieties, which reduces the accuracy of the test. (2) The testing agency needs to know the grouping trait data of the applied variety in advance, and often the applicant cannot provide accurate grouping trait data, resulting in errors in the selection of similar varieties, and the testing agency needs to spend another growth cycle. Collecting group trait data consumes a lot of time, labor and financial resources

In the applicant's follow-up research, it was found that the following problems existed in the identification of similar varieties by the above method, which needs to be further improved: (1) the locus difference method cannot accurately describe the genetic differences of different types of loci between varieties (for example, a heterozygous locus and a homozygous locus The locus difference between the points and the difference between two homozygous loci are the same when using the locus difference); (2) the judgment of the locus difference method is: the number of difference loci between varieties <3 is an approximate variety; therefore The locus difference method has extremely high requirements on the accuracy of molecular data, which requires a lot of investment in practice; (3) At the same time, the number of molecular markers used in this patent is relatively small, and the accuracy of screening similar varieties with them is poor

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