Lactic acid bacterium culture medium

Abstract

The invention discloses a lactic acid bacterium culture medium which comprises the following components: 1) 10-12g / L of peptone, 2) 4-6g / L of yeast extract, 3) 6-22g / L of glucose, 4) 2-7g / L of acid-base buffering agent, 5) 0.15-0.4g / L of growth factor, 6) 0-10g / L of beef extract, 7) 0-15g / L of soluble starch, 0-3g / L of fructose and 9) 0-20g / L of agar, wherein the pH value of the culture medium is 5.8 to 6.9. By utilizing the culture medium, the lactic acid bacterium can grow well, and moreover the problem that nearly all of the conventional commercial MRS culture media have the inbibitional effect to helicobacter pylori is solved, so that the culture medium can be used for screening lactic acid bacteria having the inbibitional effect to the helicobacter pylori, and a real and reliable screening result can be obtained in the screening experiment; and meanwhile by utilizing the culture medium, the cost of the lactic acid bacterium culture medium is greatly lowered.

Description

technical field [0001] The invention relates to a culture medium, in particular to a culture medium for lactic acid bacteria. Background technique [0002] Helicobacter pylori (Helicobacter pylori) is recognized as the main pathogenic factor of gastritis, peptic ulcer and gastric cancer. The common method for medical treatment of Helicobacter pylori infection is triple therapy using proton pump inhibitors plus two antibiotics, but due to The resulting increase in drug-resistant strains makes the curative effect decline. The research results show that some specific lactic acid bacteria can inhibit or even kill Helicobacter pylori, and can alleviate or even cure related diseases caused by Helicobacter pylori. Therefore, it has good application value in medicine and has gradually become an international research topic. hotspot. [0003] At present, at home and abroad, there have been some in vivo and in vitro experimental reports on the use of lactic acid bacteria to inhibit ...

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect that the lactic acid bacteria medium itself has an inhibitory effect on Helicobacter pylori when screening lactic acid bacteria inhibiting Helicobacter pylori in the past, thereby interfering with the defects of the screening results, and to provide a method that can guarantee the production of lactic acid bacteria Good growth medium with no inhibitory effect on Helicobacter pylori

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