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Screening method of late bolting character of radish

A screening method and technology of radish, which is applied in the field of screening of late bolting traits of radish, can solve problems such as limiting radish breeding process, achieve reliable screening results, save land resources, and the effect of simple method

Inactive Publication Date: 2003-08-06
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, radish can only bloom once a year under natural conditions, which greatly limits the radish breeding process (Chen Huoying, etc., CPPU on the influence of radish bran heart, bolting and yield in spring greenhouses, Journal of Shanghai Agricultural University, 1999, 17 (1 ), 48-51), (Wang Longzhi, Chen Huoying, etc., Four Seasons Cultivation of Radish with High Quality and High Efficiency, Science and Technology Literature Publishing House, 2001)

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] 75 from 8 materials of Bai Yuchun (from South Korea), Tianchun Dagen and Siji Xiaozheng (from Japan) and the existing domestic varieties Wuhan Chunbu Lao, Shangnong 936, Short Ye 13, 60 Days and Mid-Autumn Red Seeds (divided into 5 parts, namely 5 treatments, 15 seeds per treatment), sterilized with saturated bleaching powder solution for 20 minutes, and then soaked in sterile water at 25±1℃ for 24 hours under aseptic conditions, and germinated 2 Days; afterwards, the germinated seeds (radicles about 1-2mm in length) were inoculated into MS macroelement medium, and then 4 treated cultures of 8 materials were placed in a refrigerator with a temperature of 3±1°C Carry out low temperature treatment, and after low temperature treatment for 5 days, 10 days, 15 days, and 20 days, transfer the culture after low temperature treatment to a tissue culture room controlled at 25±1℃ and light for 16 hours a day for radish seedlings. Growth and cultivation. The other 8 materials of each ...

Embodiment 2

[0019] According to the present invention, the Korean variety Bai Yuchun, which has late bolting characteristics and excellent comprehensive agronomic traits, is used as one of the objects of localization of spring radish varieties, and the bud stage self-separation is carried out. And according to the results of the present invention, the temperature of the low temperature treatment is set to 3±1°C, and the treatment time is set to 30 days and 40 days to ensure that the seeds are received during artificial vernalization. Specific implementation plan:

[0020] On April 18th and April 28th, 1998, 10 Baiyuchun seeds were sterilized with saturated bleaching powder solution for 20 minutes, and then soaked in sterile water at 25±1℃ for 24 hours under aseptic conditions to accelerate germination. 2 days; on April 21 and May 1, inoculate the germinated seeds (radicle approximately 1mm in length) into MS macroelement medium, and then place the culture in a refrigerator with a temperature ...

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Abstract

The method for screening radish late bloting character includes the stage of vernalization and photostage, and adopts the following steps: disinfecting the seed to be identified and soaking the seed with sterile water for accelerating germination, inoculating the seed in sprouting state into MS culture medium, placing it into refrigerator, making artificial iarovization in time periods, after low-temp. treatment placing it into tissue culture chamber to make growth culture of radish seedling. In the photostage transplanting the radish seedling into the prenursery tray with matrix and placing it into tissue culture chamber and observing the bolting condition of squaring period by using different materials and making different treatments to identify the bloting characters of different materials, so that it can accelerate radish late bloting breeding process.

Description

Technical field: [0001] The invention relates to a method for screening late bolting traits of radishes, which is used for the introduction and breeding of special varieties of biennial vegetables such as radishes in spring and winter and spring, and belongs to the technical field of crop breeding. Background technique: [0002] Radish (Raphanus sativus L.) is one of the main vegetables widely cultivated in Southeast Asia. It is not only rich in nutrients, but also has high medicinal value. Although my country has a long history of cultivating radishes and rich variety resources, there is still a big gap between the research and utilization of variety resources and the advanced level of foreign countries. [0003] At present, there are few varieties of radish suitable for off-season cultivation and low quality is one of the main problems in radish production. Radish is a biennial vegetable crop, and the normal cultivation seasons are autumn, autumn and winter. The success of off-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00A01H3/02A01H4/00
Inventor 陈火英张建华朱丽萍
Owner SHANGHAI JIAO TONG UNIV
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