Screening method for species group with heterosis of germany mirror carp

A screening method and population technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., to achieve the effect of accurate screening results

Inactive Publication Date: 2009-04-08
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Selecting groups with large population differences for hybridization can reduce the workload, shorten the breeding period, reduce the cost of breeding and increase the success rate of breeding; however, how to screen the hybrid dominant populations from the numerous German mirror carp populations and ensure the genetic diversity of their hybrids , there is currently no effective method

Method used

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  • Screening method for species group with heterosis of germany mirror carp
  • Screening method for species group with heterosis of germany mirror carp
  • Screening method for species group with heterosis of germany mirror carp

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specific Embodiment approach 1

[0026] Specific embodiment one: present embodiment German mirror carp hybridization population is screened according to the following steps: a, morphological marker: the quantifiable character and the countable shape of the German mirror carp randomly selected by various populations are marked; b, again Carry out microsatellite molecular markers: (1) DNA extraction and purification; (2) RCR amplification; (3) product detection; (4) data processing; c, data analysis, select the population with large differences as the German mirror carp hybrid dominant species.

specific Embodiment approach 2

[0027]Embodiment 2: The difference between this embodiment and Embodiment 1 is that: in step a, the measurable traits include body weight, full length, body length, body height, head length, head height, tail stalk length, tail stalk height, snout Length, eye diameter and eye spacing. Other steps and parameters are the same as in the first embodiment.

specific Embodiment approach 3

[0028] Embodiment 3: The difference between this embodiment and Embodiment 1 is: in step a, the countable shapes include the left line scale, the left line side scale, the left line lower scale, the right line scale, the right scale Lateral line lateral scales and right subline scales. Other steps and parameters are the same as in the first embodiment.

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Abstract

The invention relates to a screening method of dominant hybridization species of German mirror carps and discloses a screening method of the dominant hybridization species of carps. The invention provides an effective screening method of the dominant hybridization species of the German mirror carps so as to ensure the genetic diversity of hybrids thereof, thus reducing the workloads, shortening the breeding period, decreasing the breeding cost and improving the success rate of selective breeding. The screening method includes the following steps: 1. morphological marker; 2. microsatellite molecular maker; and 3. data analysis. The method screens the dominant hybridization species of the German mirror carps by combining the morphological marker and the microsatellite molecular marker. The genetic differences between the species are dually tested and the screening results are more accurate.

Description

technical field [0001] The invention relates to a method for screening hybrid dominant populations of carp. Background technique [0002] German mirror carp (Cyprinus carpio L.mirror) is a carp species that was isolated from European wild carp after domestication due to the mutation of scales, and then artificially cultivated. Introduced to my country by the Federal Republic of Germany in 1984, and bred by the Heilongjiang Fisheries Research Institute of the Chinese Academy of Fishery Sciences for many years to F 4 It has adapted to the breeding conditions of our country and continues to show its fast-growing characteristics. It has become an excellent variety suitable for farming in northern my country, and has been recognized as a national-level fine variety by the National Original Species and Improved Variety Approval Committee. However, due to the German mirror carp breeding line (F 4 ) was selected and bred as early as 1995, and only reached the 4th generation, so so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李池陶石连玉胡雪松赵海燕贾智英
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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