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Method for screening molecular marker of Jinhu black-bone chickens and application of molecular marker

A screening method and molecular marker technology, applied in the field of genetics, can solve the problems of lack of efficient molecular biology methods, low accuracy, and high cost, and achieve the effect of abundant data, low cost, and wide application

Active Publication Date: 2019-02-12
JIANGSU INST OF POULTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a screening method for molecular markers of Jinhu Wufeng chicken, which alleviates the technical problems of high cost and low accuracy caused by the lack of efficient molecular biology methods in the current research on Jinhu Wufeng chicken

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  • Method for screening molecular marker of Jinhu black-bone chickens and application of molecular marker
  • Method for screening molecular marker of Jinhu black-bone chickens and application of molecular marker
  • Method for screening molecular marker of Jinhu black-bone chickens and application of molecular marker

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Embodiment 1

[0057] Example 1 Screening of Molecular Markers in Jinhu Wufeng Chicken

[0058] (a) Blood sample collection: 18 local chicken breeds (Langshan chicken, Anyiwa gray chicken, Gushi chicken, Xianju chicken, white-eared yellow chicken, Dongxiang green shell chicken) in Jinhu Wufeng chicken conservation group and control group laying hens, Henan gamecocks, Wenchang chickens, Daweishan miniature chickens, Tibetan chickens, Camellia chickens, ladle chickens, Huiyang bearded chickens, big bone chickens, Xiaoshan chickens, Luyuan chickens, Jinhu Wufeng chickens and Beijing oil chickens) and 2 The first introduced chicken breeds (recessive white-feathered broiler chicken and Anka red chicken) were obtained from the national-level local chicken breed gene bank of Jiangsu Institute of Poultry Science. Each group selected 30 unrelated individuals (10 males) according to the family line. , 20 mothers), 1 mL to 1.5 mL of blood was collected from the wing vein, anticoagulated with sodium cit...

Embodiment 2

[0070] Example 2 Application of Molecular Markers in Jinhu Wufeng Chicken

[0071] (a) Individual blood collection of Jinhu Wufeng Chicken Bao population: use a medical disposable syringe to collect 0.5-1.0ml of venous whole blood from male and female chicken wings randomly selected from the Bao population (no less than 10 chickens and no less than 20 hens) only), inject 2μL 0.5mol / L Na immediately after collection 2 EDTA anticoagulant in an enzyme-free tube, and then store the enzyme-free tube at 4°C for future use.

[0072] (b) DNA extraction and quality inspection: suck out 0.2 to 0.3 ml of male and hen individual blood preserved in enzyme-free tubes at room temperature, and extract individual blood DNA using conventional animal peripheral blood phenol-phenol extraction; agarose gel The DNA integrity was analyzed by electrophoresis, and the DNA purity was detected by a spectrophotometer; qualified samples were stored at -80°C for SNP detection.

[0073] (c) SNP marker det...

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Abstract

The invention provides a method for screening a molecular marker of Jinhu black-bone chickens and application of the molecular marker and relates to the field of genetics. The method for screening themolecular marker of the Jinhu black-bone chickens comprises the steps of detecting an SNP marker through high-pass sequencing, calculating genetic statistics, and analyzing and comparing genetic differences between Jinhu black-bone chicken colonies and other chicken colonies, thereby screening out the molecular marker of the Jinhu black-bone chickens. The technical problem that the research on genetic differentiation of the Jinhu black-bone chickens is high in cost and low in accuracy due to the fact that a process of evaluation on a Jinhu black-bone chicken breed conservation effect is lackof efficient molecular biology methods at present is solved, and the technical effect of efficiently and rapidly screening out the molecular marker for evaluating the Jinhu black-bone chicken breed conservation effect is achieved. The invention further provides the method for screening the molecular marker of the Jinhu black-bone chickens and application of the screened molecular marker. The technical problem that researches on the Jinhu black-bone chickens are lack of molecular biological markers and screening methods is solved.

Description

technical field [0001] The invention relates to the field of genetics, in particular to a screening method and application of molecular markers of Jinhu Wufeng Chicken. Background technique [0002] Jinhu Wufeng Chicken (Jinhu Wufeng Chicken) is native to Taining County, Fujian Province, and is a dual-purpose variety. In 2009, it passed the identification of the National Animal Genetic Resources Committee. Jinhu Wufeng Chicken has a long history of breeding. During the long-term selection and domestication process, the characteristics of "black skin, black bone and black meat" have been formed. It is a precious genetic resource with special economic value in my country's poultry bank. In recent years, with the impact of introduced species and the lack of awareness of species protection, the species is on the verge of extinction. The national-level local chicken breed gene bank was introduced in 2001 for ex-situ conservation. In order to better understand and protect this p...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/16C12Q2600/156
Inventor 李国辉韩威殷建玫张会永朱静朱云芬苏一军盛中伟沈海玉
Owner JIANGSU INST OF POULTRY SCI
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