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Method for identifying prunus persica plant species based on SSR molecular markers

A molecular marker, almond technology, applied in the molecular marker field of molecular biology, can solve problems such as difficulty in classification, cultivation and variety selection of almonds, homonymic or synonymous, unclear variety pedigree, etc.

Inactive Publication Date: 2016-04-20
XINJIANG AGRI UNIV
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Problems solved by technology

Due to long-term seed reproduction and natural evolution, abundant resources have been formed. There are nearly a hundred cultivars, strains and excellent individual plants. These resources are in different shapes and complex relationships. This phenomenon is common, which brings great difficulties to the classification, cultivation and variety selection of almonds.

Method used

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  • Method for identifying prunus persica plant species based on SSR molecular markers
  • Method for identifying prunus persica plant species based on SSR molecular markers
  • Method for identifying prunus persica plant species based on SSR molecular markers

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Embodiment

[0042] Preparation of almond germplasm materials: 5 Xinjiang wild almonds collected from different distribution populations in Tacheng and Altay, Xinjiang, China (in 5 different distribution centers of Burqin, Habahe, Tacheng, Tuoli, Randomly selected in the area, respectively numbered as Xinjiang Wild Almond No. 1, No. 2, No. 3, No. 4, and No. 5 individual plants); collected from Xinjiang local cultivated almond varieties in Yingjisha and Shache County, Kashgar region, southern Xinjiang, China 30 parts; 16 parts of excellent cultivated almond varieties imported from California, USA, collected from Shache County, Kashi District, southern Xinjiang, China; Mongolian almonds, Xikang almonds, long-stalked almonds and Yuyemei collected from the Forest Fruit Resource Garden of Xinjiang Academy of Forestry Sciences 4 copies of the germplasm resources of almonds; a total of 55 sample materials, which basically cover the germplasm resources of almonds. The samples were frozen in liquid...

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Abstract

The invention relates to a method for identifying prunus persica plant species based on SSR molecular markers. According to the method, SSR primers with high polymorphism are selected for carrying out the SSR-PCR amplified reaction, electrophoresis detection and system analysis on 55 parts of prunus persica plant specie materials. The result shows that 10 pairs of primers, with high polymorphism, screened from 100 pairs of SSR primers are used for carrying out SSR-PCR amplification on genome DNA of the prunus persica plant specie materials, 63 amplified bands are amplified together, the number of polymorphic bands is 60, the polymorphic rate is 95.2%, and the better amplification result is obtained. Similarity coefficient and cluster analysis is carried out, the genetic similarity coefficient is 0.4133-0.9600, and the average similarity coefficient is 0.6867; the genetic difference among the 55 parts of prunus persica plant specie materials shows that the different species and the genetic distances among the different species are different, when the similarity coefficient is smaller than 0.68, most cultivated varieties are clustered into one cluster, and most cultivated varieties in the same provenance can be clustered together. An important way is provided for further distinguishing the genetic relationship among the prunus persica plant specie resources.

Description

technical field [0001] The invention belongs to the field of molecular markers of molecular biology, and in particular relates to a method for identifying germplasm of almonds based on SSR molecular markers. Background technique [0002] Almond, commonly known as almond, is a plant of the genus Almond in the family Rosaceae. There are 6 species of almond germplasm resources distributed in my country, including wild and cultivated species. They are mainly distributed in the Kashgar area south of the Tianshan Mountains in Xinjiang; Mountains such as Barluk Mountain, Tarbahatai Mountain and Altai Mountain in the west of the basin; Wula Mountain and Daqing Mountain in Inner Mongolia; Helan Mountain in Ningxia; the eastern end of the Qinghai-Tibet Plateau and other places. They are common almond Amygdalus.communisL., Xinjiang wild almond Amygdalus.ledebourianaSchlecht., Mongolian almond Amygdalus.mongolicaMoxim., long handle almond Amygdalus.pedunculataPall., Xikang almond Amygdal...

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Application Information

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IPC IPC(8): G06F19/24C12Q1/68
CPCC12Q1/6858G16B40/00C12Q2531/113C12Q2535/138C12Q2565/125
Inventor 曾斌李疆田嘉夏江宏刘梦雯李伟阳刘楠楠
Owner XINJIANG AGRI UNIV
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