Influenza B virus IgA (immunoglobulin A) antibody immunofluorescence assay test strip and preparation method, detection method and application thereof

Abstract

The invention provides an influenza B virus IgA (immunoglobulin A) antibody immunofluorescence assay test strip and a preparation method thereof. The influenza B virus IgA antibody immunofluorescenceassay test strip comprises a sample pad, a label pad, a nitrocellulose membrane, a water absorbing paper and a back plate, wherein the nitrocellulose membrane comprises a detection line for influenzaB virus NP (nucleoprotein) antigen polypeptide segments, and a human IgA antibody contrast line; the label pad is provided with an anti-human IgA antibody-fluorescent microsphere marker. The influenzaB virus IgA antibody immunofluorescence assay test strip has the advantages that the detection sensitivity is very high, a high-cost PCR (polymerase chain reaction) detection instrument is not needed, the detection result can be known by naked eyes, and the influenza B virus can be quickly screened. The invention also provides a detection method and application of the influenza B virus IgA antibody immunofluorescence assay test strip. The detection method has the advantages that the steps are simple, the operation is easy, the results are quick, and the detection method is suitable for beingwidely popularized.

Description

technical field [0001] The invention belongs to the field of medical examination, and relates to a test strip for detecting influenza virus, in particular to a test strip for immunofluorescence detection of IgA antibody of influenza B virus, a preparation method, a detection method and application thereof. Background technique [0002] Every year around the world, seasonal influenza viruses are prevalent. Seasonal influenza in the population is mainly composed of influenza A virus and influenza B virus. Influenza B is a highly contagious, fast-spreading disease. It is characterized by rapid onset, chills, fever, body temperature rises to a peak within a few hours to 24 hours, and gastrointestinal symptoms are more common than other types of influenza. It is mainly spread through droplets in the air, person-to-person contact or contact with contaminated items. The antigenic mutation of influenza B virus is very slow. Influenza B virus has two strains with distinct antigeni...

AI Technical Summary

Problems solved by technology

However, since this detection technology requires an expensive PCR detector and a certified laboratory with certain detection conditions, it cannot be promoted at the grassroots level

Antigen colloidal gold immunization technology directly collects throat swabs of patients, and can judge the patient's infection with influenza virus by naked eyes; but it has the disadvantages of low sensitivity and easy to cause missed detection

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