Application of polymorphism of rs12252 in detecting influenza virus antibody

An influenza virus, polymorphism technology, applied in the biomedical field, can solve problems such as evolutionary differences

Active Publication Date: 2018-07-27
BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Previous studies have shown that IFITM3 belongs to the mouse gene family, which is a short protein (5-18kDa) containing 2 transmembrane domains, with high core sequence similarity, but evolutionary differences in the N and C-termini

Method used

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  • Application of polymorphism of rs12252 in detecting influenza virus antibody
  • Application of polymorphism of rs12252 in detecting influenza virus antibody
  • Application of polymorphism of rs12252 in detecting influenza virus antibody

Examples

Experimental program
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Embodiment 1

[0037] Example 1, IFITM3rs12252 is a single nucleotide polymorphism site related to the antibody level of young people against H1N1pdm09 influenza virus

[0038] rs12252 is a biallelic polymorphic SNP site on human chromosome 11p5.5, the variation is transition (T / C, A / G on its complementary strand), and the site is located in the IFITM3 gene. The rs12252 genotype was CC, CT or TT. Among them, CC is the homozygous type whose rs12252 site is C, TT is the homozygous type whose rs12252 site is T, and CT is the heterozygous type whose rs12252 site is T and C.

[0039] 1. Subjects and samples

[0040] 99 young healthy volunteers, aged 17-22 years, were neither infected with influenza virus nor vaccinated against influenza within 3 months.

[0041] Genomic DNA is extracted from each individual.

[0042] 2. Genotyping of rs12252 locus

[0043] (1) Amplify the nucleotide fragment containing the SNP site

[0044] Primers were designed according to the flanking sequence of rs12252,...

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Abstract

The invention discloses an application of polymorphism of rs12252 in detecting an influenza virus antibody. The technical scheme protected by the invention comprises that provided is an application ofa substance, used for detecting the polymorphism or genotype of the rs12252 in a human genome, in preparation of products for detection or assisted detection of the level of an antibody, aiming at H1N1pdm09 influenza virus, in young people, an application in preparation of a product for screening young people suggested to be inoculated with a vaccine for preventing influenza caused by the H1N1pdm09 influenza virus, or an application in preparation of a product for detecting the resistibility of young people on the H1N1pdm09 influenza virus. The substance can be used for screening the young people suggested to be injected with the vaccine for preventing the influenza caused by the H1N1pdm09 influenza virus.

Description

technical field [0001] The invention relates to the application of the polymorphism of rs12252 in the detection of influenza virus antibody in the field of biomedicine. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) refers to the variation of a single nucleotide in the genome, which is the smallest variation unit, and is a variation formed by a single nucleotide pair substitution, transversion, insertion or deletion form. Single nucleotide polymorphism is a high-density genetic marker on the genome, and the number of SNPs found in the human genome exceeds 30 million. As a third-generation genetic marker, SNPs are numerous, densely distributed, and easy to detect, so they are ideal genotyping targets. [0003] rs12252 is a biallelic polymorphic SNP site on human chromosome 11p5.5. IFITM3 gene. [0004] The gene of interferon-induced transmembrane protein 3 (IFITM3, also known as 1-8U) was initially identified in 1984. It...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156
Inventor 覃岭张永宏赵艳李宁
Owner BEIJING YOUAN HOSPITAL CAPITAL MEDICAL UNIV
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