Amino-modified water-soluble fluorescent quantum dot and preparation method thereof

Abstract

The invention relates to an amino-modified water-soluble fluorescent quantum dot and a preparation method thereof. The water-soluble fluorescent quantum dot is of a multi-layer core-shell structure, an oil-soluble fluorescent quantum dot (such as CdSe / ZnS) is adopted as a core, the surface of the oil-soluble fluorescent quantum dot is subjected to a silanization rection, so that the lipophilic and hydrophobic properties of the quantum dots are modified into hydrophily in one step; furthermore, an amino group (-NH2) is adopted, so that surface amino modification for the water-soluble quantum dot is finished; the prepared amino-modified water-soluble quantum dot has an average size of 10nm and is uniform in size, has a wide excitation spectrum continuously distributed in a range of 250-602nm, and has good emission spectrum monochromaticity with a maximum emission wavelength of 634nm and fluorescence-emission half peak width of 43nm. The amino-modified quantum dot is good in biocompatibility, can be coupled with various bioactive substances, and has great practical value and application prospect in the biology and medicine fields such as immunofluorescence diagnose, cell imaging and living imaging.

Description

technical field [0001] The invention belongs to the technical field of advanced nanocomposite materials and biochemical analysis, and relates to an amino-modified water-soluble fluorescent quantum dot and a preparation method thereof. Background technique [0002] The full name of quantum dots is semiconductor quantum dots (SemiconductorQuantumDots, referred to as quantum dots), which are nanoparticles composed of II-VI or III-V elements, and the particle size range is generally 2~20nm. Among them, CdX is mainly studied. (X=S, Se, Te). As a new type of luminescent material, compared with traditional organic dye molecules, the biggest advantage of quantum dots lies in its rich colors. A single type of quantum dot can produce a family of markers with different emission wavelengths and distinct colors according to the change of particle size, which is simply impossible for dye molecules. In addition, its excitation spectrum is wide and continuously distributed, and its emissi...

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Problems solved by technology

And the patent adopts a two-step method, firstly SiO 2 Silylation modified to obtain SiO 2 The silanized product is then modified with functional groups containing amino, carboxyl, and mercapto groups. The preparation method is the reverse emulsion method (water-in-oil method), and the preparation time is as long as 1 to 6 days, and the preparation efficiency is low.

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