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Rare-earth carbon nanoparticle, preparation method of rare-earth carbon nanoparticle and application for determining pH value based on fluorescence color scale

A carbon nanoparticle and nanoparticle technology, applied in the field of luminescence inspection, can solve the problems of complex organic chemical synthesis of molecular probes, large size of nanoparticle fluorescent probes, insoluble molecular probes in water, etc., and avoid complex chemical synthesis. , The effect of rapid pH response and high sensitivity

Active Publication Date: 2018-12-07
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among the pH measurement methods reported, the method of detecting pH with synthetic molecular probes is mostly difficult to dissolve in water, so it needs to use organic solvents to help dissolve them, and the preparation of molecular probes requires complex organic chemical synthesis. ; Nanoparticle fluorescent probes are easy to prepare, but most of the nanoparticle fluorescent probes are large in size, and it is difficult to enter the inside of the cell to measure the pH value

Method used

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  • Rare-earth carbon nanoparticle, preparation method of rare-earth carbon nanoparticle and application for determining pH value based on fluorescence color scale
  • Rare-earth carbon nanoparticle, preparation method of rare-earth carbon nanoparticle and application for determining pH value based on fluorescence color scale
  • Rare-earth carbon nanoparticle, preparation method of rare-earth carbon nanoparticle and application for determining pH value based on fluorescence color scale

Examples

Experimental program
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Embodiment 1

[0041] Embodiment 1 Preparation of Rare Earth Carbon Nanoparticles C:EuTbDPA

[0042]Add 0.5mmol europium nitrate, 0.5mmol terbium nitrate and 0.5mmol 2,6-pyridinedicarboxylic acid to 10mL polyethylene glycol 400 reagent in order to form polyethylene glycol 400: europium ion: terbium ion: 2,6-pyridine dicarboxylic acid The molar ratio of carboxylic acid is a mixed solution of 55:1:1:1; the reactant is stirred at 80°C for 10 minutes, then the temperature is raised to 100°C and stirred for 20 minutes, and finally stirred at 210°C for 6 minutes, and the heating equipment is terminated. The whole reaction needs to be carried out under nitrogen as protective gas. After cooling down to room temperature, add acetone solution and centrifuge to collect the pale yellow precipitate, then centrifuge and wash the precipitate twice with acetone and deionized water at a rotational angular speed of 14000 rad / s, and wash for 10 min each time, and finally dry the precipitate at 60°C Backup. f...

Embodiment 2

[0043] The preparation of embodiment 2 rare earth carbon nanoparticles C:EuTbDPA

[0044] Add 0.4mmol europium nitrate, 0.4mmol terbium nitrate and 0.5mmol 2,6-pyridinedicarboxylic acid to 10mL polyethylene glycol 400 reagent in order to form polyethylene glycol 400: europium ion: terbium ion: 2,6-pyridine dicarboxylic acid The molar ratio of carboxylic acid is a mixed solution of 55:0.8:0.8:1; the reactant is stirred at 80°C for 10 minutes, then the temperature is raised to 110°C and stirred for 20 minutes, and finally stirred at 210°C for 6 minutes to stop the heating equipment. The whole reaction needs to be carried out under nitrogen as protective gas. After cooling down to room temperature, add acetone solution and centrifuge to collect the pale yellow precipitate, then centrifuge and wash the precipitate twice with acetone and deionized water at a rotational angular speed of 14000 rad / s, and wash for 10 min each time, and finally dry the precipitate at 60°C Backup. The...

Embodiment 3

[0045] Preparation of Example 3 Rare Earth Carbon Nanoparticles C:EuTbDPA

[0046] Add 0.4mmol europium nitrate, 0.5mmol terbium nitrate and 0.5mmol 2,6-pyridine dicarboxylic acid to 10mL polyethylene glycol 400 reagent in order to form polyethylene glycol 400: europium ion: terbium ion: 2,6-pyridine dicarboxylic acid The molar ratio of carboxylic acid is a mixture of 55:0.8:1:1.1; the reactant is stirred at 80°C for 10 minutes, then the temperature is raised to 120°C and stirred for 30 minutes, and finally stirred at 230°C for 8 minutes, and the heating equipment is terminated. The whole reaction needs to be carried out under nitrogen as protective gas. After cooling down to room temperature, add acetone solution and centrifuge to collect the pale yellow precipitate, then centrifuge and wash the precipitate twice with acetone and deionized water at a rotational angular speed of 14000 rad / s, and wash for 10 min each time, and finally dry the precipitate at 60°C Backup. The a...

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Abstract

The invention discloses a rare-earth carbon nanoparticle, a preparation method of the rare-earth carbon nanoparticle and application for determining a pH value based on a fluorescence color scale. Therare-earth carbon nanoparticle disclosed by the invention is a fluorescent nanoparticle formed by carrying out a one-pot hydrothermal reaction among rare-earth europium ion, terbium ion as well as 2,6-dipicolinic acid and polyethylene glycol 400. The fluorescence color scale principle of the rare-earth ions is changed to indicate the pH value by utilizing the enhanced luminescence effect of carbon dots and protonation of the 2,6-dipicolinic acid. The rare-earth carbon nanoparticle disclosed by the invention can give out light to indicate the pH value in the aqueous solution and / or cells, hasexcellent fluorescence in the aqueous solution, high pH determination sensitivity and fast pH response, and have advantages to determination of the pH value of a biological sample with excellent fluorescence background. Compared with the general visual colorimetry according to the fluorescence color scale, the method for determining the pH value based on the fluorescence color scale is accurate.

Description

technical field [0001] The invention relates to a rare-earth carbon nanoparticle, a preparation method thereof and an application of measuring pH value based on fluorescence chromaticity. The nanoparticle is a pH value indicating material and belongs to the technical field of luminescence inspection. Background technique [0002] pH measurement is a basic task that is widely used in various production processes, daily life, environmental protection, and scientific research. pH balance is particularly important to organisms, because protein functions have corresponding pH requirements, and the occurrence and development of many diseases are closely related to pH changes in the body. Conventional pH measurement includes indicator method, glass electrode method, etc. These methods are not suitable for pH measurement in biological systems such as cells. Fluorescent molecular probes can be applied to pH measurement in biological systems. Some fluorescent molecules measure pH The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C09K11/06B82Y20/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y40/00C09K11/06C09K11/65C09K2211/182G01N21/6486
Inventor 陈扬王路得
Owner SOUTHEAST UNIV
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