80results about How to "Efficient and fast detection" patented technology

Methods and constructs for selecting double-stranded RNA molecules capable of post-transcriptional gene silencing (PTGS) or RNA interference (RNAi); and methods of selecting targets susceptible to double-stranded RNA mediated PTGS or RNAi.

The invention provides a method for detecting a pseudo base station based on a short message center number. The method comprises the steps of firstly, detecting a short message from a base station and identifying the short message center number of the short message, secondly, determining whether the short message center number accords with a predetermined identification rule, and if so, determining that the short message is from the pseudo base station. The method for detecting the pseudo base station based on the short message center number is capable of quickly determining whether the short message is from the pseudo base station by judging the short message center number of the received short message from the base station, and therefore, quick and efficient detection on the pseudo base station is realized.

The invention discloses a method for identifying whether tea is irradiated and irradiation absorbed dose and a new application of an electronic nose. The identifying method comprises: employing tea samples which are treated by irradiation of different known doses; detecting the tea samples whose irradiation absorbed doses are known by the electronic nose; drawing a response diagram of tea volatile substances detected by the electronic nose; determining an identification evaluation time; calculating the variance contribution rate of an electronic nose sensor characteristic constant of each main component and the correct rate of initial group case return discrimination, and preferably choosing an effective sensor; calculating to obtain classification function coefficients of different radiation doses, and obtaining a classification function for identifying a tea sample radiation dose; detecting an unknown tea sample by the electronic nose, calculating with the classification function, and obtaining a radiation dose of the tea. Compared with the prior art for identifying tea, the identifying method provided by the invention has the characteristics of simple method, few steps, fast speed, high identification efficiency, high accuracy, etc., and the method is suitable for identifying whether the tea is irradiated or the irradiation absorbed dose.

The invention provides two biological resistive gas sensors made from perylene bisimide derivatives and a method for manufacturing the biological resistive gas sensors. Perylene of the biological resistive gas sensors contains different substituting groups. Source regions and drain regions of the sensors are made from elementary gold; channel regions of the sensors comprise single micrometer wires or nanowires made from two types of perylene bisimide derivatives; and gate dielectric layers of the sensors are made from silicon and silicon dioxide. The biological gas sensors made from the two types of perylene bisimide derivatives are high in sensitivity when in hydrazine hydrate atmosphere, and variation of the sensitivity of each sensor is closely related to molecular structures. The biological resistive gas sensors and the method have the advantages that the hydrazine hydrate atmosphere can be efficiently and quickly detected, the sensors are simple in structure and small in size, and are portable, and the biological resistive gas sensors and the method have a high application prospect in amine gas detection.

The invention discloses a hardware-in-the-loop experimental platform of an electric control air suspension vehicle height adjusting system and an adjusting method. The hardware-in-the-loop experimental platform comprises a vehicle body, a measurement and control system and a drum test bench, wherein the vehicle body comprises an air compressor, an inflation electromagnetic valve, a plurality of air springs and a deflation electromagnetic valve which are connected in sequence; and the measurement and control system comprises a height sensor, a data acquisition card, a host machine and a targetmachine. A test method of the hardware-in-the-loop experimental platform based on the vehicle height adjusting system comprises vehicle height static control and vehicle height dynamic control, a hostmachine and target machine dual-machine simulation environment is set, a target height is formulated, the vehicle height static control utilizes the height sensor to acquire the current vehicle height, a signal enters a vehicle height control model along with the data acquisition card, a vehicle height control unit controls the on and off of the electromagnetic valves through decision-making whenthe height of a vehicle body changes, so that the height of the vehicle body is recovered, the vehicle height dynamic control simulates a road surface by means of the drum test bench and inputs a height control command by means of a monitoring interface of the host machine, thereby realizing hardware-in-the-loop simulation testing.

The present invention relates to the technical field of network security, in particular to a method for judging UDPFlood attack and defense based on binary tree detection, comprising the following steps: 1. Construct a binary tree structure to store and query UDP data packets and related information; 2. UDP Pre-store data packets and related information; 3. Search the binary tree, and compare the information stored in the binary tree with the currently analyzed information to determine whether it is a UDP Flood attack; 4. Call the operating system firewall program to filter and send UDP Flood Host IP, refuses to receive packets. The present invention can achieve the following beneficial effects: the large-scale DDoS-type UDP Flood attack adopts a binary tree-based cycle detection method, pre-stores UDP data packets through the binary tree and searches the binary tree for matching and cycle detection, and the detection is fast, efficient, and highly accurate , has an excellent defensive effect.

The invention discloses a battery diaphragm surface defect detection method. The method comprises the following steps of: modeling an acquired product image of a standard battery diaphragm; based on the modeling, obtaining a modeling result comprising a template image and a to-be-detected area; according to the determined to-be-detected area, comparing the to-be-detected product image with the template image to obtain an original defect list; based on defect types and position information, aggregating the obtained original defect list to obtain an aggregated defect list; and based on the aggregated defect list, outputting defects meeting a preset threshold in the aggregated defect list. According to the structural design of the method, the surface defects of a battery diaphragm can be efficiently and quickly detected; the method has the advantages of high efficiency, low cost, high accuracy, and can effectively detect the defects such as coating missing and spraying missing. In addition, the invention also discloses a battery diaphragm surface defect detection system.

A method and a device for fast amplification and detection of target nucleotide sequences possibly present in a sample, combine preferably PCR amplification with oligochromatographic detection by capillarity on a test strip.

The invention provides a perylene bay 1,7-bit nitro-containing perylene bisimide derivative. The perylene bay 1,7-bit nitro-containing perylene bisimide derivative is used for preparing a perylene bay 1,7-bit nitro-containing perylene bisimide derivative microwire, and is used for preparing a gas-sensitive element of a resistor-type gas-sensitive sensor; in the prepared gas-sensitive element, a material forming a source zone and a drain zone is simple substance gold, the material for forming a channel region is the perylene bay 1,7-bit nitro-containing perylene bisimide derivative microwire, and the material for forming a gate medium layer is silicon and silica. The gas-sensitive element used in the ammonia gas detection has the advantages that the response and recovery time is short, the detection limit is low, the resistor-type gas-sensitive sensor is simplified and minimized, the carrying is facilitated, and the effective and rapid detection of ammonia gas is achieved.

The invention provides a perylene bisimide derivative containing S-configuration camphorsulfonic acid and a preparation method thereof. The perylene bisimide derivative containing S-configuration camphorsulfonic acid is used for preparing perylene bisimide derivative microwires containing the s-configuration camphorsulfonic acid, and is used for preparing a gas-sensitive element of a resistive gas sensor; in the prepared gas sensor, a source region and a leakage region are prepared from metallic gold, a channel region is prepared from the perylene bisimide derivative microwires containing the s-configuration camphorsulfonic acid, and a gate dielectric is prepared from silicon and silicon dioxide. The gas sensor prepared by the invention is applied to detection of amine gas, has the advantages of short response and recovering time and low detection limit, capability of simplifying and reducing the resistive gas sensor, convenience in carrying, is used for realizing efficient rapid detection of the amine gas, and has wide application prospect in the detection of high boiling point amine gas.

The present invention relates to polynucleotides enabling the rapid, simple and specific detection of Group B Streptococcus highly-virulent ST-17 clones. The present invention also relates to the polypeptides encoded by said polynucleotides, as well as to antibodies directed or raised against said polypeptides. The present invention also relates to kits and methods for the specific detection of Group B Streptococcus highly-virulent ST-17 clones, using the polynucleotides, the polypeptides or the antibodies according to the invention.

The invention provides a railway track defect detection method and system. The method is applied to a railway track defect detection system. The method comprises the following steps: preprocessing anoriginal rail image to obtain a first rail image; inputting the first rail image into a rail area positioning module for processing; determining left and right boundaries of the track in the originaltrack image according to the output of the track area positioning module; segmenting a first rail image from the original rail image according to the left and right boundaries; preprocessing the firsttrack image to obtain a second track image; utilizing a preset window to slide on the second track image at a preset step length, and inputting the area image placed in the preset window into a defect classification module to obtain a defect detection result; and according to a defect detection result, framing the detected defect area in the second track image. Whether the rail has defects or notand defect types can be quickly and accurately recognized.

A process of in vivo labeling and identifying recombinantly produced peptides or proteins within an unpermeabilized prokaryotic host cell. Recombinant prokaryotic cells expressing a fusion peptide comprising at least one tetracysteine tag were labeled in vivo using a biarsenical labeling reagent. A fluorescent activated cell sorter was used to identify and select subpopulations of fluorescent cells wherein the amount of fusion peptide in the cell was proportional to the amount of fluorescence detected.

The invention discloses a fluorescence detection primer of culex wolbachia, a detection method thereof and a detection kit. The fluorescence detection primer is characterized by comprising wPipF: 5minute-GTTTGTGCAGCTAATAG-3minute; wPipR: 5minute-GTCTGCAAGGCCT ATTTCTACTG-3minute and a probe: 5minute-CTTTCAATTGAAAAGATTCGATCAAC-3minute, wherein the two ends of the probe are respectively combined with a fluorescence generation group and a fluorescence quenching group. The culex wolbachia can be detected out in a fast-efficient-single manner by utilizing the fluorescence detection primer and the probe according to the detection method, and the fluorescence detection primer has the characteristics that the singleness is strong, the specificity is high (the culex wolbachia can be detected out, but no amplification of the aedes wolbachia occurs), the sensitivity is high and the lowest detection limit is 100copies per ml. Therefore, the fluorescence detection primer disclosed by the invention has the advantages of fastness, high efficiency, simple operation, high specificity, high sensitivity and simple identification and the like.

The invention discloses crack detection equipment with an easy-to-open cover, and relates to the technical field of crack detection. The easy-open cover crack detection equipment comprises a bottom plate device, a clamping device is arranged above the rear end of the bottom plate device, the clamping device comprises a first plate, the rear end of the lower surface of the first plate is fixedly connected with a first stand column, the lower end of the first stand column is fixedly connected with the bottom plate device, the front end of the first plate is fixedly connected with a first clamp, and the front end of the upper surface of the first clamp is concavely provided with a first groove; the rear end of the upper surface of the first clamp protrudes outwards to form a first protruding layer, a second plate is arranged above the first plate, the front end of the second plate is movably connected with a second clamp through a spring shaft, the front end of the lower surface of the second clamp protrudes outwards to form a second protruding layer, the rear end of the lower surface of the second clamp is concavely provided with a second groove, and the first clamp and the second clamp jointly clamp the easy-open cover. And the detection device comprises a detection column I. The crack detection equipment for the easy-to-open cover can know the position of a crack and the depth of an indentation and detect the crack of the easy-to-open cover.

The invention discloses an automatic detecting device for a high voltage box tooling. The device comprises: a power module, which is used for supplying power to an internal network circuit of a high voltage box; a test instrument, which is used for collecting a loop voltage value, a loop current value and a loop resistance value in a preset detection loop, and then transmitting the values to a host computer through a CAN card; and an upper computer, which is connected to the test instrument through the CAN card, and is used for receiving the loop voltage value, the loop current value and the loop resistance value in the preset detection loop transmitted by the test instrument and then judging whether there is an abnormal condition in the preset detection loop, and displaying the judgment result on a display screen of the upper computer. The automatic detecting device for a high voltage box tooling disclosed by the invention can efficiently and quickly detect the working performance ofa high voltage box, thereby improving the detection efficiency, saving high manpower and material resources, and thus reducing the overall production cost of the high voltage box, which is beneficialfor a wide range of applications and has great practical significance in production.

The present invention relates to the field of biotechnology, in particular relates to a Brucella outer membrane protein antibody detection ELISA (enzyme-linked immuno sorbent assay) kit. The ELISA kit uses Brucella outer membrane protein OMP28 expressed by escherichia coli as antigen for package of an enzyme label plate, Brucella reference positive serum and negative serum are respectively used as a positive control and negative control, Goat Anti-Bovine IgG labeled with HRP (horseradish peroxidase) is used as an enzyme labeled antibody, and the ELISA kit is assembled by combination of a dilution liquid, a washing liquid, a substrate solution a, a substrate solution B and a termination liquid. The Brucella outer membrane protein antibody detection ELISA kit has the advantages of being safe, sensitive, specific, simple, fast and stable, can meet inspection and monitoring epidemic prevention requirements of Brucella antibody in cattles in China.

The invention discloses a visual inspection method for underwater culvert inner wall image defects. The method comprises the following steps: acquiring an underwater culvert defect image by shooting via an image sensor, the detection of the underwater culvert defect image being divided into image preprocessing, image coarse extraction and image accurate extraction; to be specific, carrying out dodging processing and image enhancement preprocessing operation on an image, and dividing a preprocessed defect image into non-overlapping image blocks with the same size; forming a gray feature space of the defect image by utilizing gray features of the image blocks, and realizing coarse extraction of cracks based on k-means clustering; and marking all defect connected domains, analyzing geometricdimension characteristics of the connected domains to form a connected domain characteristic space, and realizing accurate extraction of underwater culvert inner wall image defects based on Gaussian mixture model soft clustering. According to the method, accurate extraction of the underwater culvert inner wall image defects is achieved through the visual inspection method, detection is rapid and free of damage, and maintenance of the underwater culvert is more economical and reasonable.

The invention provides a guide rail inspecting device and a hydraulic straightening machine. The guide rail inspecting device comprises a guide rail to be inspected, locating blocks, a straightening instrument, computer equipment, a guide rail sliding base, a reflector base and a reflector. The guide rail to be inspected is arranged on the locating blocks, the straightening instrument is arranged at one side of the guide rail to be inspected and is in communication connection with the computer equipment, and the straightening instrument is used for inspecting data of the guide rail to be inspected and sending the data to the computer equipment for data processing. The guide rail sliding base is arranged on the guide rail to be inspected, and the reflector base is connected with the guide rail sliding base so as to slide along the guide rail to be inspected through the guide rail sliding base. The reflector is connected with the reflector base in a rotating mode and used for reflecting incident light rays sent by the straightening instrument and adjusting a light path sent by the light path. The guide rail inspecting device can inspect the guide rail to be inspected rapidly and efficiently, time and labor are saved, the automation degree is high, and the labor intensity of workers is reduced.

The invention relates to a sensing device of a vitamin detector, which belongs to the technical field of electrochemical detection. The sensing device of the vitamin detector is characterized in that the sensing device is provided with a three-electrode sensor connecting seat, two auxiliary electrodes and one active electrode, the active electrode and the auxiliary electrodes are arranged on the three-electrode sensor connecting seat, and the active electrode and the auxiliary electrodes are connected with lead connecting sheets and connected to a detection circuit through a shielding line. The sensing device of the vitamin detector is suitable for electrochemical detection, and uses three electrodes for sensing, so as to perform steady signal detection on samples; and the detector has the advantages of high sensitivity, excellent accuracy, high automation degree, compact overall structure and the like, is simple and convenient for operation, and can quickly and effectively detect vitamin of blood samples.