34 results about "Wolbachia" patented technology

A modified arthropod, an arthropod-modifying bacterium, and use thereof as an agent for control of diseases transmitted by arthropods, particularly mosquitoes, is provided. More specifically, an isolated arthropod-adapted Wolbachia bacterium capable of modifying one or more biological properties of a mosquito host is provided. The modified arthropod may be characterized as having a shortened life-span, a reduced ability to transmit disease, a reduced susceptibility to a pathogen, a reduced fecundity, and / or a reduced ability to feed from a host, when compared to a corresponding wild-type arthropod.

The invention discloses a method for quickly and effectively transfecting mosquito by using wolbachia. According to the method, an insects ovarian tissue (abundant sources and easiness for obtaining) containing the wolbachia is directly acquired by adopting a vivisection method and is homogenated; and the ovarian tissue is separated from the wolbachia by using a centrifugation method so as to obtain a purified usable wolbachia transfected by embryonic injection. The embryo of the mosquito is injected and transfected by the purified wolbachia through a conventional method; a primary female adult insect (G0) is obtained by egg culture; the obtained primary female adult insect is mated with a wild type male mosquito to generate first generation (G1) mosquito strains; and positive mosquito strains containing the wolbachia are screened from the first generation mosquito strains and become really and successfully-transfected mosquito strains. Therefore, the method for transfecting the mosquito by using the wolbachia is a quick and effective method. The realization of the invention is beneficial to quickly establishing the mosquito strains transfected with the wolbachia and further beneficial to large-scale application of the mosquito strains.

The invention relates to a multiple PCR detection method for detecting Wolbachia and Cardnium symbiotic bacterias in tetranychus truncatus ehara body at the same time, belonging to the field of agricultural biotechnologies; the multiple PCR is an improved PCR technology on the basis of a common PCR, a plurality of pairs of auele specific primers are added in a PCR reaction system, aiming at different areas of a plurality of DNA templates or the same template, a plurality of purpose fragments are amplified. According to Wolbachia wsp gene sequence and Cardnium 16S rDNA gene sequence, two pairs of new auele specific primers are designed, and two purpose fragments - 211bp and 279bp which has different size, are amplified. The multiple PCR detection method has high specificity, sensibility, feasibility and effectiveness, namely, the detecting cost is saved and the template is saved. The multiple PCR detection technology for detecting the Wolbachia and Cardnium symbiotic bacterias at the same time can be widely applied to the detection of the two symbiotic bacterias in tetranychus truncatus ehara.

The invention provides compositions and methods for the detection and quantification of Wolbachia antibodies, antibody fragments, and polypeptides.

The invention discloses a method for extracting wolbachia used for embryonic injection transfection, which comprises: dissecting an insect having wolbachia to obtain the ovary of the insect, and placing the ovary in sucrose phosphate glutamate (SPG) buffer solution; and homogenizing the ovary, washing the homogenate with the SPG buffer solution, collecting the homogenate, placing the collected homogenate in a centrifuge to centrifuge the homogenate in amount of 100 to 1,000 grams for 1 to 10 minutes, collecting supernate, centrifuging the supernate in an amount of 5,000 to 15,000 grams for 5 to 20 minutes, removing supernate, washing the sediment with the SPG buffer solution to obtain suspension, centrifuging the suspension in an amount of 100 to 1,000 grams for 1 to 10 minutes, collecting the supernate which is pure wolbachia, wherein the pure wolbachia can be used for embryonic injection in mosquitoes. In the method disclosed by the invention, the source (ovary tissues of insects having the wolbachia) of the wolbachia is rich and can be obtained easily; and the whole method is simple and convenient in operation, has very high controllability, and can obtain lots of wolbachia forembryonic injection transfection in a give time very easily, thereby saving lots time and energy, improving working efficiency and contributing to quick establishment of a mosquito strain transfectedwith wolbachia.

The invention discloses a detection primer of culex wolbachia, a detection method thereof and a detection kit. The detection primer is characterized by comprising wPipF: 5 minute-GTGCAGCTAATAGCAAGGAACA-3minute;and wPipR: 5 minute-CCTATCTTTTCTGGCATCTTCG-3 minute. The culex wolbachia can be detected out in a fast-efficient-single manner by utilizing the detection primer according to the detection method, and the detection primer has the characteristics that the singleness is strong, the specificity is high (the culex wolbachia can be detected out, but no amplification of the aedes wolbachia occurs), the sensitivity is high and the lowest detection limit is 855 times 10<-5> ng per mul. Therefore, the detection primer disclosed by the invention has the advantages of fastness, high efficiency, simple operation, high specificity, high sensitivity and simple identification and the like.

The invention discloses a rice planthopper egg treatment method for microinjection. The method comprises the following steps that fresh rice planthopper eggs are collected; according to the principle that the heads and the tails of the eggs are in the same directions respectively, the eggs are sequentially arranged and transferred to a glass slide with an double-sided adhesive, the eggs are dried for 2-3 min, and then mosquito eggs are immediately covered with water-saturated oil to prevent further drying and water loss of the mosquito eggs; microinjection is conducted, the rice planthopper eggs obtained after injection are reserved after microinjection is completed, the rice planthopper eggs and the double-sided adhesive are put into pure water together, and culture is conducted for 2-3 days; when the rice planthopper eggs enter the red-eye period, the rice planthopper eggs are gently picked out and placed on wet filter paper, 2 days later, whether larvae are incubated or not is judged under an anatomical lens, and then the incubated larvae are transferred to tender shoots of rice seedlings. According to the method, natural non-infected wolbachia brown planthopper eggs serve as microinjection receptors, the infection rate is increased by 5-20%, and the egg passage efficiency is also improved by 80-100%.

The invention discloses a method for extracting wolbachia used for embryonic injection transfection, which comprises: dissecting an insect having wolbachia to obtain the ovary of the insect, and placing the ovary in sucrose phosphate glutamate (SPG) buffer solution; and homogenizing the ovary, washing the homogenate with the SPG buffer solution, collecting the homogenate, placing the collected homogenate in a centrifuge to centrifuge the homogenate in amount of 100 to 1,000 grams for 1 to 10 minutes, collecting supernate, centrifuging the supernate in an amount of 5,000 to 15,000 grams for 5 to 20 minutes, removing supernate, washing the sediment with the SPG buffer solution to obtain suspension, centrifuging the suspension in an amount of 100 to 1,000 grams for 1 to 10 minutes, collecting the supernate which is pure wolbachia, wherein the pure wolbachia can be used for embryonic injection in mosquitoes. In the method disclosed by the invention, the source (ovary tissues of insects having the wolbachia) of the wolbachia is rich and can be obtained easily; and the whole method is simple and convenient in operation, has very high controllability, and can obtain lots of wolbachia forembryonic injection transfection in a give time very easily, thereby saving lots time and energy, improving working efficiency and contributing to quick establishment of a mosquito strain transfectedwith wolbachia.

The invention discloses a detection primer of culex wolbachia, a detection method thereof and a detection kit. The detection primer is characterized by comprising wPipF: 5 minute-GTGCAGCTAATAGCAAGGAACA-3minute;and wPipR: 5 minute-CCTATCTTTTCTGGCATCTTCG-3 minute. The culex wolbachia can be detected out in a fast-efficient-single manner by utilizing the detection primer according to the detection method, and the detection primer has the characteristics that the singleness is strong, the specificity is high (the culex wolbachia can be detected out, but no amplification of the aedes wolbachia occurs), the sensitivity is high and the lowest detection limit is 855 times 10<-5> ng per mul. Therefore, the detection primer disclosed by the invention has the advantages of fastness, high efficiency, simple operation, high specificity, high sensitivity and simple identification and the like.

Compositions and Methods are described in which Albendazole sulfone binds to Wolbachia FtsZ providing anti filarial activity.

The present invention provides systems, methods, and compositions for identifying a subject as infected with a parasite by detecting nucleic acid from an endosymbiont of the parasite in a sample from the subject. In certain embodiments, the parasite is a nematode that infects humans or dogs (e.g., D. immitis, O. volvulus, W. bancrofti, B. timori, or B. malayi) and the endosymbiont is Wolbachia.

The invention discloses the application of sulfadiazine to the reproductive behavior of Trichogramma brevis infected with Wolbachia. The invention provides the application of sulfadiazine in eliminating symbiotic Wolbachia or reducing the titer of Wolbachia in animals. The present invention finds for the first time that the honey water containing 1.0mg / ml sulfadiazine alone is used to feed the short-tube Trichogramma, which can well remove the Wolbachia carried by the short-tube Trichogramma. This method is more convenient, time-saving, and obtains complete male offspring. This will lay the foundation for the further use of Wolbachia to regulate the reproductive mode of arthropods, and has good application value and promotion significance.

The invention discloses a preparation method of aedes albopictus stably carrying culex quinquefasciatus wolbachia and the application of the aedes albopictus stably carrying the culex quinquefasciatus wolbachia to prevention and control of the aedes albopictus. The preparation method comprises the following steps that Guangzhou wild aedes albopictus treated with tetracycline are screened, so that aedes albopictus without wolbachia is obtained, and the aedes albopictus stably carrying the culex quinquefasciatus wolbachia is prepared. The application of the aedes albopictus stably carrying the culex quinquefasciatus wolbachia to prevention and control of the aedes albopictus has the advantages that the cost is low, and sustainability and environment friendliness are achieved. The aedes albopictus stably carrying the culex quinquefasciatus wolbachia is one of the most potential biological measures for prevention and control of mosquito-borne diseases, the problem that insects can easily establish drug resistance under the condition of using chemical insecticides is solved, and the problem that harm to the environment and the public health is caused is also solved.

Compositions and Methods are described in which Albendazole sulfone binds to Wolbachia FtsZ providing anti filarial activity.

The invention discloses a primer for quickly and quantitatively detecting Wolbachia in tissues of a mosquito and a reagent box and a method thereof. According to the invention, different tissues containing Wolbachia are separated and obtained to extract Wolbachia genomic DNA; and then the Wolbachia DNA is subjected to Real-time PCR (Polymerase Chain Reaction) detection by using a specific PCR primer of Wolbachia. The realization of the invention is beneficial for quickly and quantitatively analyzing a large quantity of tissue samples containing Wolbachia, so that the distribution of Wolbachiain the tissues of the mosquito can be conveniently monitored in a large territorial scope on a large scale.

The present invention disclosed a test and identified insect body Wolbachia The specific primers, methods and kits of infection.TW16S1, which is special amplifier, includes positive primer F and reverse primer R, and its sequences are shown in sequel: 1-2.The detection method is: S1. Extract the total DNA of insects to be tested; S2. With S1 DNA as a template, using the primer to perform the PCR amplification reaction; S3.EssenceThe primers described by the present invention are strong, the amplification products are a single destination fragment, and the sensitivity is high. It can screen a false negative and non -specific sample, thereby increasing the detection rate.It only takes about 4 hours to obtain the results from mentioning DNA.You can accurately and quickly detect whether to detect whether the sample is subject to Wolbachia Infection provides a basis for further use of disease prevention and reproductive regulation of insects.