6 results about "Wolbachia" patented technology

The application provides a method for preventing and treating monochamus alternatus, which comprises putting male monochamus alternatus carrying Wolbachia into a target prevention and treatment area of monochamus alternatus not carrying Wolbachia. The method has low investment cost, is green, safe, environment-friendly, has no pesticide residue and high risk, conforms to the green prevention and control principle and needs of diseases and pests in the new era, is less affected by external temperature, humidity and other climate environments, is suitable for wide plots, is a new prevention and treatment method, is early in the prevention and treatment period, prevents and treats from the egg stage, reduces the double hazards of the larval stage dry woodworm and the pupal stage adult stage spreading pine wood nematode disease from the root, has good prevention and treatment effect, and can significantly reduce the egg hatching rate of monochamus alternatus by more than 90%.

The invention relates to the technical field of microbial cells, and discloses a method for transfecting a symbiotic bacterium Wolbachia into a lepidoptera insect cell line for culture, which comprises the following steps: collecting insect eggs infected with a Wolbachia host; culturing a lepidoptera insect cell line; adding eggs of the insect infected with the Wolbachia host into a centrifugal tube containing PBS (Phosphate Buffer Solution); washing the eggs; adding a cell culture medium into the centrifugal tube after the eggs are washed, and fully grinding the eggs by using a sterilization grinding rod to obtain an egg homogenate; according to the method, bacteria and insect cells are tightly combined together by utilizing weak centripetal force generated by low-speed centrifugation, and the bacteria are absorbed into the cells by combining the endocytosis and exocytosis characteristics of the cells, so that the cross-host transfection of the symbiotic bacteria is realized. The technology is of great significance to establishment of new insect strains infected with specific symbiotic bacteria.

The invention discloses a method for distributing and positioning a symbiotic bacterium Wolbachia wLui in liriomyza sativae in different insect states. The method comprises the following steps: firstly, collecting each insect-state individual; secondly, a fluorescence labeling specific probe primer of the Wolbachia wLhui strain is synthesized; then, hybridizing Wolbachia wLhui strains in the eggs, the larvae, the pupae and the male and female adult bodies by adopting a specific probe; and finally, observing and shooting the tissue distribution of the Wolbachia wLhui strain in the bodies of different insect states of the liriomyza sativae by using a fluorescence confocal microscope. The method disclosed by the invention has the characteristics of specificity, sensitivity, effectiveness, simplicity in operation and low cost, provides important technical support for interaction research of Wolbachia wLhui strains and liriomyza sativae, and provides reference for rapid positioning of symbiotic bacteria in various insect states of other liriomyza sativae.

The present disclosure is directed to materials and method for genetically modifying Wolbachia, as well as arthropods comprising the modified Wolbachia.

The invention provides a culex neo-wolbachia subtype as well as a detection primer, a detection method and a detection kit thereof. The invention discloses a novel Wolbachia subtype, and designs a specific detection primer, a probe and a detection method aiming at a gene segment of the novel Wolbachia. By utilizing the detection primer and the probe disclosed by the invention, the neowolbachia culex subtype can be rapidly, efficiently and specifically detected according to the detection method disclosed by the invention. The method has the advantages of rapidness, high efficiency, simplicity and convenience in operation, high specificity, high sensitivity and the like.

The application discloses a skin-biomimetic composite microporous membrane and a manufacturing process thereof. The skin-biomimetic composite microporous membrane comprises, from top to bottom, a woven mesh layer, a cracked PU film layer and an adsorption layer. The woven mesh layer is provided with mesh holes through which mouth parts of mosquitoes can pass. The cracked PU film layer has cracks and can be pierced by the mouth parts of mosquitoes. The adsorption layer is used for adsorbing mosquito feeding liquid. The woven mesh layer is used for supporting the cracked PU film layer, improving the structural strength of the microporous membrane, and reducing the occurrence of wrinkling, deformation and the like. The cracks of the cracked PU film layer can allow a small amount of mosquito feeding liquid to pass through, so as to emit an odor to attract mosquitoes. After the mosquitoes approach, the cracked PU film layer is pierced by the mouth parts, so that the mosquito feeding liquid adsorbed by the adsorption layer is sucked, so that the purposes of attracting mosquitoes and feeding mosquitoes are achieved. The application facilitates the cultivation of mosquitoes containing Wolbachia, reduces human work, reduces labor cost, and has a better feeding effect.