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Fluorescent cell markers

A marker and cell technology, applied in animal cells, vertebrate cells, artificial cell constructs, etc.

Inactive Publication Date: 2014-01-01
KODE BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006]Cellular labeling by binding of fluorophores to surface-expressed proteins may affect cellular function

Method used

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Embodiment Construction

[0065] The specification of International Application PCT / NZ2005 / 000052 (publication number WO 2005 / 090368) describes a water-soluble synthetic molecule, which is F-S 1 -S 2 -L structure.

[0066] In these structures, F is a carbohydrate that is spontaneously and stably incorporated into the lipid bilayer that includes the cell membrane.

[0067] Preferred structures described in the specification of this international application include substructures:

[0068]

[0069] wherein n=3 to 5, X is H or C, and * is not H.

[0070] Typically, M is H, but can also be replaced by another monovalent cation such as Na + 、K + or NH 4 + substitute.

[0071] F is a fluorophore in the structure of the present invention, which has different physicochemical properties from carbohydrates. Select the spacer (S 1 -S 2 ) to provide a structure that is readily dispersible in an aqueous vehicle, such as saline.

[0072] Without wishing to be bound by theory, it is believed that the ce...

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Abstract

The preparation and use of fluorescent cell markers of the structure F—S1-S2-L is described where F is a fluorophore, S1-S2 is a spacer linking F to L, and L is a diacyl lipid.

Description

technical field [0001] The present invention relates to fluorescent cell markers. The present invention particularly relates to fluorescent cell markers comprising a fluorophore of fluorescein, BODIPY or one of their derivatives. Background technique [0002] The compounds fluorescein, BODIPY, and their derivatives contain fluorophores. [0003] Fluorescein is water soluble. The use of fluorescein as a cell marker requires its binding to reactive groups such as isothiocyanates. The isothiocyanate group of fluorescein isothiocyanate (FITC) can react with the amine group of protein. [0004] FITC is used to label cells by binding to surface expressed proteins. Labeled cells can then be sorted by fluorescence-activated cell sorting (FACS). [0005] The fluorophore of BODIPY has favorable spectral properties over that of fluorescein. Derivatives of BODIPY are also used to label cells by binding to surface expressed proteins. [0006] Cell labeling by binding of fluoropho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C07F5/02C12N5/071C07D311/78C07F9/09G01N33/53
CPCC09K2211/1011G01N33/92C09K2211/1029C09K11/06G01N33/582C09K2211/1088C09K2211/1044C07F9/6561C07F9/65522G01N33/5005G01N2458/00C07F9/6596C09K2211/1048C09K2211/1055G01N33/80
Inventor 叶连娜·科尔恰金娜尼乔莱·博温斯蒂芬·亨利
Owner KODE BIOTECH
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