Fluorescence-patch clamp-micropipette detection device

Abstract

The invention discloses a fluorescence-patch clamp-micropipette detection device. An experimental accommodating cavity, a three-dimensional micromanipulator and a patch clamp are arranged on an experiment platform, and two sides of the experimental accommodating cavity are hollowed out for a micropipette and a glass electrode to enter; fluorescent cells are contained in the experimental accommodating cavity, the micropipette sucks red blood cells, the fluorescent cells and the red blood cells are positioned in an extracellular fluid in the experimental accommodating cavity, and the glass electrode is filled with an electrode fluid; the patch clamp comprises a patch clamp amplifier, a patch clamp probe and a patch clamp probe bracket; full-spectrum light emitted from the fluorescence lightsource passes through a color filter to form fluorescence incident light, the fluorescence incident light enters the cells in the experimental accommodating cavity, and fluorescence of the cells returns to be received by means of a fluorescence camera. According to the fluorescence-patch clamp-micropipette detection device, the influence of the cell membrane potential change on the membrane protein interaction can be detected while a high signal-to-noise ratio fluorescence image is collected to research transmembrane signal transduction of the membrane protein, and the coupling relationship among the three spectrum phase information can be recorded while the fluorescence spectrum, the electrophysiological spectrum, the adhesion state spectrum and other three spectrum phase information aresynchronously recorded.

Description

technical field

[0001] The invention relates to the integration of fluorescence imaging technology, patch clamp technology, and micropipette technology, and in particular to a detection device capable of studying membrane protein transmembrane signal transduction while detecting the regulation of membrane potential on the interaction between protein molecules. Background technique

[0002] The membrane potential of the cell membrane is a key regulator of cell life activities. The membrane potential of neurons plays an important role in regulating the life activities of neurons, and is an important biophysical factor that dynamically regulates the structure and function of brain neural networks; in non-neuronal cells, the membrane potential of cells also regulates their proliferation , differentiation and other life activities. Patch clamp technology is an effective means to study membrane potential-related issues, and its whole-cell recording mode can realize accurate and r...

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