Two-parameter dynamic fluorescent detecting system and method thereof

A fluorescence detection, dual-parameter technology, applied in the field of fluorescence detection systems, can solve problems such as high cost and complex system, achieve fast dynamic detection, complex operation process, and realize the effect of dual-parameter synchronous rapid detection

Active Publication Date: 2019-04-19
SHANGHAI JIAO TONG UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Although applicable to a variety of measured substance components, each detection process can only measure a single parameter, and the system is complex and costly

Method used

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  • Two-parameter dynamic fluorescent detecting system and method thereof
  • Two-parameter dynamic fluorescent detecting system and method thereof
  • Two-parameter dynamic fluorescent detecting system and method thereof

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Embodiment

[0058] Such as Figure 1-3 It is a schematic structural diagram of a preferred embodiment of a dual-parameter dynamic fluorescence detection system of the present invention, as figure 1 As shown, the figure includes: box 1, light source 2, emission filter 3, cuvette 4, first photomultiplier tube 5a, second photomultiplier tube 5b, first receiving filter 6a, second Receiving filter 6b, power supply 7, data collector 8, instrument cover 9, light-shielding cover 10.

[0059] Such as figure 1 , image 3 As shown, a schematic structural diagram of a dual-parameter dynamic fluorescence detection device of the present invention, such as figure 2 As shown, the box body 1 is a sealed box body, and the interior of the box body 1 is divided into six chambers, the upper central chamber is used to install the light source 2, and the two chambers on the left and right sides of the upper part are used to install the power supply 7 respectively. With the data collector 8, the chamber in ...

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Abstract

The invention provides a two-parameter dynamic fluorescent detecting system and a method thereof. The system comprises a light source, a transmitting optical filter, a colorimetric ware, a first photomultiplier, a second photomultiplier, a first receiving optical filter, a second receiving optical filter and a data collector. The light source, the transmitting optical filter and the colorimetric ware form a fluorescent exciting light path. After monochromatic light excitation, a quantum dot and a sensitive substance respectively excite two fluorescent signals with different wavelengths. The fluorescent intensities of the two different wavelengths are respectively detected through the first photomultiplier and the second photomultiplier, thereby realizing synchronous quick measurement of the two parameters. The two-parameter dynamic fluorescent detecting system and the method thereof have advantages of small device size and small weight.

Description

technical field [0001] The invention relates to a fluorescence detection system and method, in particular to a dual-parameter dynamic fluorescence detection system and method. Background technique [0002] Among the optical detection methods, the inherent detection sensitivity of the fluorescence method is high, the amount of sample required is small, and the detection limit is usually lower than other methods. A variety of measurement parameters are available, so it has a wide range of applications. At present, fluorescence method has been widely used in biochemistry, chemistry, drug analysis, food inspection, geography, metallurgy, medicine, environmental science, life science research and other fields. [0003] At present, the instruments based on the fluorescence method mainly include fluorescence analyzers and fluorescence spectrophotometers, both of which can determine the properties and contents of fluorescent substances. The fluorescence analyzer is mainly a tool f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 陶卫赵辉吕娜王侃郑超刘权胡艳丽夏润之
Owner SHANGHAI JIAO TONG UNIV
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