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Self-driven micro-fluidic chip with integrated processing and amplified color developing functions

A microfluidic chip, self-driven technology, applied in the field of microbial detection, can solve problems such as expensive detection costs, and achieve the effect of simplifying the detection operation process

Pending Publication Date: 2019-12-31
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fully automated all-in-one Real-time PCR instruments have been commercialized (e.g., GeneXpert, Cepheid); however, high testing costs associated with high-precision instruments remain a barrier to large-scale adoption

Method used

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  • Self-driven micro-fluidic chip with integrated processing and amplified color developing functions
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  • Self-driven micro-fluidic chip with integrated processing and amplified color developing functions

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Embodiment 1

[0047] A self-driven microfluidic chip with integrated processing and amplification and color development functions, which is composed of several pathogen detection monomers; the various liquid pools on the pathogen detection monomer are connected by channels, and the channels are controlled by nanometers through shielding technology. Material build. Such as figure 1 and figure 2 As shown, the self-driven microfluidic chip can be provided with a total of 7 pathogen detection monomer structures with 6 sample combinations. The sample liquid pool of each monomer structure includes sample pool 1, nucleic acid denaturation pool 2, amplification detection pool 3 and overflow pool 4 arranged in sequence according to the liquid flow direction; Passage 5 for nucleic acid extraction. The structural surface of the nucleic acid extraction channel 5 is molecularly modified using the principle of solid-phase reversible immobilization (SPRI). Seven amplification detection pools 3 surrou...

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Abstract

The invention belongs to the technical field of microbiological detection and particularly relates to a self-driven micro-fluidic chip with integrated processing and amplified color developing functions. The chip is formed by combining a plurality of pathogen detecting monomers; sample liquid pools on the pathogen detecting monomers communicate with one another through channels; and the channels are constructed by nanometer materials and by a shielding technology. The self-driven micro-fluidic chip can perform amplification directly from tissue lysis fluid at one step, can effectively reduce the sample detection cost, can improve the detection efficiency, can guarantee the detection accuracy and can improve the epidemic disease screening ability of shrimp seed industry fundamentally.

Description

technical field [0001] The invention belongs to the technical field of microorganism detection, and in particular relates to a self-driven microfluidic chip with integrated processing and amplification and color development functions. Background technique [0002] Judging from the current situation and trend of the development of disease prevention and control of prawn farming in my country, it is the key point to promote the technological upgrading and structural adjustment of my country's prawn farming industry under the guidance of biosecurity, and the development of biosecurity work requires a larger sample size and more pathogens. Accurate, sensitive, and low-cost point-of-care testing (POCT) technology. An ideal POCT analysis integrates sample preparation, amplification, and detection, and can get rid of the limitations of the detection environment. Polymerase chain reaction (PCR) is the current standard for high-sensitivity molecular detection, however, it is not suit...

Claims

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Application Information

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IPC IPC(8): C12M1/40C12M1/38C12M1/34C12M1/00C12Q1/70C12Q1/6893C12Q1/6844
CPCB01L3/502707B01L3/5027C12Q1/701C12Q1/6893C12Q1/6844B01L2300/0864B01L2300/0887B01L2300/166B01L2400/0406C12Q2565/629
Inventor 曹志黄倢董宣邹培卓万晓媛李晨
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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