The invention provides a method for separating X sperms and Y sperms, belongs to the field of
livestock breeding. The method comprises the following steps: diluting a latex
stock solution, adding a
buffer solution and a pancreatin solution, uniformly mixing, carrying out water bath overnight, centrifuging to remove supernatant, and adding the
buffer solution to obtain a latex suspension; and diluting an NT-4
antibody, carrying out water bath, dropwise adding the NT-4
antibody into the latex suspension, carrying out water bath, and placing at
room temperature to obtain an
antibody latex solution, and taking
semen of a male
mammal, adding a
semen diluent, uniformly mixing, adding the antibody latex solution, uniformly mixing, and carrying out water bath incubation, wherein the upper half-layer solution in the incubation product contains Y sperms, and the
lower half-layer solution in the incubation product contains X sperms. According to the invention, the method does not generate
side effect, does not affect
maternal health, does not reduce
semen quality, reduces the malformation rate, is stable in separation effect and suitable for wide popularization and application, is simple and convenient to operate, improves the
sperm separation efficiency, and can greatly reduce the
workload of operators, save manpower, and reduce the labor cost.