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Method for separating X sperms and Y sperms

A technology for sperm and semen, applied in the field of animal husbandry, can solve problems such as high cost and difficult operation, and achieve the effects of reducing deformity rate, improving separation efficiency and stable separation effect.

Pending Publication Date: 2022-02-25
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods have the disadvantages of difficult operation and high cost, and are not suitable for widespread application.

Method used

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  • Method for separating X sperms and Y sperms
  • Method for separating X sperms and Y sperms
  • Method for separating X sperms and Y sperms

Examples

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Effect test

Embodiment 1

[0043] The separation method of embodiment 1 bull X sperm and Y sperm

[0044] Dilute the 10% polystyrene latex microsphere stock solution to 2% with distilled water, add boric acid buffer solution and 1% trypsin solution and mix well, place in a water bath at 45°C overnight, centrifuge at 2000-3000g, discard the supernatant, and then Add boric acid buffer to prepare 1% latex suspension;

[0045] Dilute the NT-4 antibody to 50-100ug / ml with PBS solution, place it in a 56°C water bath for 30 minutes, take 0.2ml of the NT-4 antibody dilution and slowly add it dropwise to 1ml of 1% latex suspension, gently Shake gently to promote adsorption, then place it in a 56°C water bath for 2 hours, shake it 2-3 times in the middle, and then place it at room temperature for 4 hours to obtain the antibody latex solution;

[0046] Take 1ml bull semen and add it to 4ml semen diluent, mix well, then add 0.2ml antibody latex solution, mix well, and incubate in 30℃ water bath for 30min;

[0047...

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Abstract

The invention provides a method for separating X sperms and Y sperms, belongs to the field of livestock breeding. The method comprises the following steps: diluting a latex stock solution, adding a buffer solution and a pancreatin solution, uniformly mixing, carrying out water bath overnight, centrifuging to remove supernatant, and adding the buffer solution to obtain a latex suspension; and diluting an NT-4 antibody, carrying out water bath, dropwise adding the NT-4 antibody into the latex suspension, carrying out water bath, and placing at room temperature to obtain an antibody latex solution, and taking semen of a male mammal, adding a semen diluent, uniformly mixing, adding the antibody latex solution, uniformly mixing, and carrying out water bath incubation, wherein the upper half-layer solution in the incubation product contains Y sperms, and the lower half-layer solution in the incubation product contains X sperms. According to the invention, the method does not generate side effect, does not affect maternal health, does not reduce semen quality, reduces the malformation rate, is stable in separation effect and suitable for wide popularization and application, is simple and convenient to operate, improves the sperm separation efficiency, and can greatly reduce the workload of operators, save manpower, and reduce the labor cost.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry, and in particular relates to a method for separating X sperm and Y sperm. Background technique [0002] In recent years, with the advancement of science and technology and the continuous deepening of research, in order to reduce production costs and obtain greater production benefits in animal husbandry production, in addition to adopting reasonable nutrition methods and good feed management technologies, producers Attention has been set to controlling the sex of domestic animals. Livestock sex control refers to the technology of intervening by artificial means to make female breeding livestock breed offspring of a specific sex according to people's wishes. As far as animal husbandry is concerned, breeding farms that aim to promote breeding stock hope to produce more male animals, while farms that focus on economic interests, especially dairy and poultry farms, hope to obtain more femal...

Claims

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Application Information

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IPC IPC(8): C12N5/076
CPCC12N5/061C12N2509/00
Inventor 李纯锦周虚袁晨丰曹茂盛张博琦
Owner JILIN UNIV
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