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Assembled type multifunctional sensor device and preparation method thereof

A sensor device and multi-functional technology, which is applied in the field of component multi-functional sensor devices based on functional particles and its preparation, can solve the problems of low flux, key technologies that need to be developed, and restrictions on wide application

Inactive Publication Date: 2006-11-22
张爱华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its main problems include low throughput and key technologies that still need to be developed, which limit its wide application.

Method used

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  • Assembled type multifunctional sensor device and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0046] First, the polystyrene particles with a tumbler structure containing magnetic particles with a uniform size and a diameter of about 50 microns on the upper surface and a circular surface with a diameter of about 50 microns are obtained by the casting method, and then the upper surface is treated with nitrogen plasma gas to make it There are amino groups on the surface, and then the hepatitis A antibody is linked with the linking reagent glutaraldehyde to obtain a sensing functional unit capable of detecting the hepatitis A antigen. Repeat the above steps to prepare polystyrene sensing functional units capable of detecting hepatitis B and hepatitis C in batches with square and diamond-shaped tumbler structures on the surface and embedded magnetic particles. Put one of the above three sensing functional units into a microbox with a transparent upper cover and a flat lower bottom with a diameter of about one centimeter, and react with human serum samples under magnetic stir...

Embodiment 2

[0048] First prepare silica microspheres with a diameter of 10 microns in batches through the microsphere synthesis method, and then immerse them in 1% aminopropyltrihexyloxysilane ethanol solution to react for 2 hours, take them out and clean them, and connect them in batches with glutaraldehyde Anti-avidin and one of the monoclonal antibodies against influenza B virus, monoclonal antibody against influenza A virus and monoclonal antibody against influenza C virus, and then the zinc selenide quantum dot particles with different spectral responses were labeled After the avidin reacts with the above three microspheres and washes them, the three microspheres are put into a detachable centrifuge tube cover embedded in a microcavity, and the microcavity has micropores facing the inside of the centrifuge tube. Inject the solution of the blood sample to be analyzed and the three kinds of fluorescent-labeled influenza virus polyclonal antibodies into the centrifuge tube in which the a...

Embodiment 3

[0050] First prepare silica microspheres with a diameter of 10 microns containing superparamagnetic magnetic particles in batches by the microsphere synthesis method, then immerse them in 1% aminopropyl trihexyloxysilane ethanol solution and react for 2 hours, then take them out for cleaning Afterwards, glutaraldehyde was used to connect anti-avidin to one of the monoclonal antibodies to influenza B virus, monoclonal antibody to influenza A virus and monoclonal antibody to influenza C virus in batches, and then labeled with different spectral responses. The avidin of the zinc selenide quantum dot particle reacts with the above three microspheres and washes them, and injects the three microspheres into a microfluidic chip through an injection hole. Inject the solution of the blood sample to be analyzed and the three kinds of fluorescently labeled polyclonal antibodies against influenza virus through another injection hole and carry out magnetic stirring and mixing reaction at an...

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Abstract

The disclosed assembly-type multifunction sensor fit for large-scale production comprises: a mobile sensing unit (1) arranged on a sensing energy-conversion platform (2), a control device (3) on side of (3) better on bottom, and an auxiliary unit. This invention also discloses the preparation method.

Description

technical field [0001] The invention relates to a sensor and a preparation method thereof, in particular to a sensor for synchronously detecting biological and chemical substances in large quantities, in particular to a component-type multifunctional sensor based on functional particles and a preparation method thereof. Background technique [0002] Multifunctional sensor devices capable of detecting multiple materials at the same time are the development direction of biological and chemical sensor devices due to their small size, large amount of information, good performance, convenient use, and high reliability. of high attention. At present, multifunctional sensor devices for biological and chemical materials are mainly concentrated on array sensor chips and microfluidic chips. The array sensor chip mainly detects a variety of biological and chemical substances by reacting with samples after preparing an array of sensing sensitive materials by spotting and in-situ synthe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N31/00
Inventor 张爱华张继中
Owner 张爱华
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