Method and device for quantitative PCR multi-wavelength fluorescence detection

A fluorescence detection, multi-wavelength technology, used in fluorescence/phosphorescence, microbial determination/inspection, biochemical equipment and methods, etc. Effect

Active Publication Date: 2010-05-12
HANGZHOU BIOER TECH CO LTD
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Problems solved by technology

If the excitation light is the adjacent well, the excitation light will be transmitted to the adjacent well through the test tube made of transparent material, ...

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  • Method and device for quantitative PCR multi-wavelength fluorescence detection
  • Method and device for quantitative PCR multi-wavelength fluorescence detection

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Embodiment Construction

[0020] Further illustrate the present invention below in conjunction with accompanying drawing.

[0021] refer to figure 1 , the multi-wavelength fluorescence detection device for quantitative PCR of the present invention comprises n fluorescence wavelength detection channels spaced apart from each other as a standard hole position of a module, a polyhedron mirror 11 and an optical sensor 12, in the illustrated example, n=2, polyhedron reflection The mirror 11 has two working surfaces, and each fluorescence wavelength detection channel has a light source excitation fiber path 1 and a fluorescence receiving fiber path 2. In this example, the light source excitation fiber and the fluorescence receiving fiber adopt Y-shaped bifurcated fibers. A light source 3, a first lens 4, a first optical filter 5, and a first focusing mirror 6 are sequentially arranged on the light source excitation fiber path along the light source excitation direction, and a second lens 7, The second filte...

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Abstract

The invention discloses a method and a device for quantitative PCR multi-wavelength fluorescence detection. N fluorescence wavelength detection passages with the mutual interval as a module standard hole site are arranged, each fluorescence wavelength detection passage is provided with a light source exciting optical fiber path and a fluorescence receiving optical fiber path, the light sources of the n light source exciting optical fiber paths respectively pass through a collimating lens and an exciting light filter so as to obtain exciting light with required wavelength, and then the exciting light is irradiated onto n test tube exciting reagent samples through focusing; the fluorescence of the n test tube exciting reagent samples respectively enters the fluorescence receiving optical fiber paths, the fluorescence with the required wavelength is obtained through the collimating lens and the exciting light filter, is irradiated onto a plane reflecting mirror and is then refracted onto n working surfaces of the same polyhedral reflecting mirror through the plane reflecting mirror, and the fluorescence of the n fluorescence wavelength detection passages is converged onto a photoelectric surface of an optical sensor through the polyhedral reflecting mirror so as to realize the real-time synchronous detection of the multi-wavelength fluorescence. The invention can greatly improve the detection efficiency, no exciting light interference is generated in detection, and quantitative PCR analysis results are exact.

Description

technical field [0001] The invention relates to a multi-wavelength fluorescence detection method for quantitative PCR and a device thereof. Background technique [0002] The working principle of the quantitative PCR detection system is an instrument that automatically cycles the reactants between the specified denaturation temperature, annealing temperature and extension temperature. Through the temperature cycle of denaturation, annealing and extension, the target DNA can be amplified by millions in a short time. times. [0003] The quantitative PCR detection system irradiates the test tubes with different wavelengths of excitation light. When the reagents in the test tubes are excited to emit fluorescence of specific wavelengths, optical sensors, such as PMT (photomultiplier tube), PD (photosensitive diode), APD (avalanche photodiode) ) and so on to collect the fluorescence intensity signal and send it to the computer in time for real-time data display and analysis. [0...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 刘志华项伟平吕才树王勇李社刚
Owner HANGZHOU BIOER TECH CO LTD
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