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Method for evaluating soil health through quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms

A technology for analysis and evaluation, microorganisms, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as time-consuming, high requirements for testing equipment and experimental techniques, and inability to obtain information on microorganism types, saving time and consumption less effect

Inactive Publication Date: 2011-02-16
FUDAN UNIV
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Problems solved by technology

Although the use of molecular biology methods (such as: construction of clone library, denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP), etc.) Not the same, the detection equipment and experimental technology require high requirements, take a long time, and have certain limitations
Although the method of constructing a clone library can obtain accurate sequence information, the disadvantage is that a large amount of sequencing is required for each sample, and the cost required is relatively high.
The advantage of the DGGE method is the simultaneous analysis and comparison of multiple samples, but the disadvantage is poor reproducibility and the inability to perform direct comparisons between different analyses.
Although the T-RFLP method has good repeatability, it cannot obtain information about the type of microorganisms corresponding to each peak

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  • Method for evaluating soil health through quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms
  • Method for evaluating soil health through quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms
  • Method for evaluating soil health through quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms

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Embodiment Construction

[0024] The tidal flat ecosystem is an important ecological barrier in the coastal economic belt. Healthy tidal flats are an important guarantee for the sustainable development of my country's offshore aquaculture industry and the basis for the protection of fishery resources. Due to the siltation of river runoff, more than 500,000 mu of land is newly formed every year. Therefore, using tidal flats to reclaim land to expand the cultivated land area has become the main measure to expand the cultivated land area in coastal areas, and tidal flats have become an important reserve land resource in my country. As one of the three major estuaries in my country, the Yangtze Estuary is one of the main distribution areas of muddy tidal flats in my country. The rich sediment carried by the runoff of the Yangtze River was deposited near the mouth, forming a vast tidal flat wetland. It is very important to determine the degree of soil development in time during the reclamation of tidal fla...

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Abstract

The invention belongs to the technical field of biological detection, and in particular relates to a method for evaluating soil health through the quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms. The method of the invention comprises the following steps of: synthesizing a suitable quantitative PCR primer according to microorganism parameters for evaluating the soil health; constructing standard product plasmids of all the parameters and establishing a standard curve; and extracting sample DNA (Deoxyribonucleic Acid) and detecting the copy numbers of all the types of microorganism genes in the samples through quantitative PCR so as to judge the soil health state. The invention integrates various molecular biology methods needed for evaluating the soil health till now into a whole and is rapid, simple, convenient and effective.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a method for evaluating soil health through quantitative PCR analysis of various genes of various microorganisms. Background technique [0002] Soil quality refers to the comprehensive measurement of three aspects: soil fertility quality, soil environmental quality and soil health quality. Within the scope of the ecosystem, soil maintains biological production capacity, protects environmental quality and promotes the health of animals and plants. In order to monitor the changes of cultivated land soil quality, many countries and regions have been or are working on the construction of cultivated land soil quality monitoring methods and monitoring systems in the region. In this regard, Canada is leading the way. Since 1989, Canada has included monitoring changes in agricultural soil health status in the Ministry of Agriculture's national benchmark project. The baseline ...

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/06
Inventor 全哲学孟晗任文伟李壳
Owner FUDAN UNIV
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