Method for evaluating soil health through quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms

A technology for analysis and evaluation, microorganisms, applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as time-consuming, high requirements for testing equipment and experimental techniques, and inability to obtain information on microorganism types, saving time and consumption less effect

Inactive Publication Date: 2011-02-16
FUDAN UNIV
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of molecular biology methods (such as: construction of clone library, denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP), etc.) Not the same, the detection equipment and experimental technology require high requirements, take a long time, and have certain limitations
Although the method of constructing a clone library can obtain accurate sequence information, the disadvantage is ...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for evaluating soil health through quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms
  • Method for evaluating soil health through quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms
  • Method for evaluating soil health through quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] The tidal flat ecosystem is an important ecological barrier in the coastal economic belt. Healthy tidal flats are an important guarantee for the sustainable development of my country's offshore aquaculture industry and the basis for the protection of fishery resources. Due to the siltation of river runoff, more than 500,000 mu of land is newly formed every year. Therefore, using tidal flats to reclaim land to expand the cultivated land area has become the main measure to expand the cultivated land area in coastal areas, and tidal flats have become an important reserve land resource in my country. As one of the three major estuaries in my country, the Yangtze Estuary is one of the main distribution areas of muddy tidal flats in my country. The rich sediment carried by the runoff of the Yangtze River was deposited near the mouth, forming a vast tidal flat wetland. It is very important to determine the degree of soil development in time during the reclamation of tidal fla...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of biological detection, and in particular relates to a method for evaluating soil health through the quantitative PCR (Polymerase Chain Reaction) analysis of microorganisms. The method of the invention comprises the following steps of: synthesizing a suitable quantitative PCR primer according to microorganism parameters for evaluating the soil health; constructing standard product plasmids of all the parameters and establishing a standard curve; and extracting sample DNA (Deoxyribonucleic Acid) and detecting the copy numbers of all the types of microorganism genes in the samples through quantitative PCR so as to judge the soil health state. The invention integrates various molecular biology methods needed for evaluating the soil health till now into a whole and is rapid, simple, convenient and effective.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to a method for evaluating soil health through quantitative PCR analysis of various genes of various microorganisms. Background technique [0002] Soil quality refers to the comprehensive measurement of three aspects: soil fertility quality, soil environmental quality and soil health quality. Within the scope of the ecosystem, soil maintains biological production capacity, protects environmental quality and promotes the health of animals and plants. In order to monitor the changes of cultivated land soil quality, many countries and regions have been or are working on the construction of cultivated land soil quality monitoring methods and monitoring systems in the region. In this regard, Canada is leading the way. Since 1989, Canada has included monitoring changes in agricultural soil health status in the Ministry of Agriculture's national benchmark project. The baseline ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12Q1/06
Inventor 全哲学孟晗任文伟李壳
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap