Method and device for quantitative PCR multi-wavelength fluorescence detection

Abstract

The invention discloses a method and a device for quantitative PCR multi-wavelength fluorescence detection. N fluorescence wavelength detection passages with the mutual interval as a module standard hole site are arranged, each fluorescence wavelength detection passage is provided with a light source exciting optical fiber path and a fluorescence receiving optical fiber path, the light sources ofthe n light source exciting optical fiber paths respectively pass through a collimating lens and an exciting light filter so as to obtain exciting light with required wavelength, and then the exciting light is irradiated onto n test tube exciting reagent samples through focusing; the fluorescence of the n test tube exciting reagent samples respectively enters the fluorescence receiving optical fiber paths, the fluorescence with the required wavelength is obtained through the collimating lens and the exciting light filter, is irradiated onto a plane reflecting mirror and is then refracted onton working surfaces of the same polyhedral reflecting mirror through the plane reflecting mirror, and the fluorescence of the n fluorescence wavelength detection passages is converged onto a photoelectric surface of an optical sensor through the polyhedral reflecting mirror so as to realize the real-time synchronous detection of the multi-wavelength fluorescence. The invention can greatly improve the detection efficiency, no exciting light interference is generated in detection, and quantitative PCR analysis results are exact.

Description

technical field [0001] The invention relates to a multi-wavelength fluorescence detection method for quantitative PCR and a device thereof. Background technique [0002] The working principle of the quantitative PCR detection system is an instrument that automatically cycles the reactants between the specified denaturation temperature, annealing temperature and extension temperature. Through the temperature cycle of denaturation, annealing and extension, the target DNA can be amplified by millions in a short time. times. [0003] The quantitative PCR detection system irradiates the test tubes with different wavelengths of excitation light. When the reagents in the test tubes are excited to emit fluorescence of specific wavelengths, optical sensors, such as PMT (photomultiplier tube), PD (photosensitive diode), APD (avalanche photodiode) ) and so on to collect the fluorescence intensity signal and send it to the computer in time for real-time data display and analysis. [0...

AI Technical Summary

Problems solved by technology

If the excitation light is the adjacent well, the excitation light will be transmitted to the adjacent well through the test tube made of transparent material, forming a background and causing false excitation, and its influence is more than 10% of the typical fluorescence signal intensity, which cannot meet the requirements

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