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Screening method and application of reference genes in real-time fluorescent quantitative PCR (Polymerase Chain Reaction) analysis of plum blossom

A real-time fluorescence quantitative, internal reference gene technology, applied in the field of garden plant molecular biology, can solve problems such as expression level changes

Active Publication Date: 2022-05-10
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the expression levels of the current internal reference genes may vary in different varieties, tissues and flowering stages

Method used

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  • Screening method and application of reference genes in real-time fluorescent quantitative PCR (Polymerase Chain Reaction) analysis of plum blossom
  • Screening method and application of reference genes in real-time fluorescent quantitative PCR (Polymerase Chain Reaction) analysis of plum blossom
  • Screening method and application of reference genes in real-time fluorescent quantitative PCR (Polymerase Chain Reaction) analysis of plum blossom

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[0036] 1 Materials and methods

[0037] 1.1 Experimental materials

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Abstract

The invention discloses application of GAPDH, UBQ, Actin7, PP2A and Actin1 genes as reference genes and special primers thereof in real-time fluorescent quantitative PCR (Polymerase Chain Reaction) analysis of plum blossom. The invention also discloses a screening method of the reference gene in real-time fluorescent quantitative PCR analysis of plum blossom, which can provide theoretical basis and technical guarantee for molecular level research of plum blossom.

Description

technical field [0001] The invention relates to the field of molecular biology of garden plants, in particular to a screening method and application of internal reference genes in real-time fluorescent quantitative PCR analysis of plum blossoms. Background technique [0002] Plum blossom (prunus mume) is a perennial woody plant of the genus Prunus in the family Rosaceae. It is native to southern China and has been cultivated for more than 3,000 years. It is a famous ornamental tree species. With the deepening of plum blossom genome sequencing and molecular research, the selection of suitable internal reference genes will provide an important guarantee for further research on the formation of important agronomic traits and molecular breeding of plum blossoms. [0003] Real-time fluorescence quantitative PCR (qRT-PCR) has the characteristics of high accuracy, high sensitivity, strong specificity, and wide detection range. It has great significance for the study of gene express...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6851C12N15/11
CPCC12Q1/6895C12Q1/6851C12Q2600/166C12Q2545/101C12Q2531/113C12Q2563/107C12Q2561/113Y02A50/30
Inventor 董彬赵宏波叶勇王艺光杨丽媛肖政钟诗蔚方遒
Owner ZHEJIANG FORESTRY UNIVERSITY
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