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Phaeodactylum tricornutum culture medium

A technology of Phaeodactylum tricornutum and culture medium, applied in the field of Phaeodactylum tricornutum culture medium, can solve problems such as culture failure, and achieve the effects of increased algal cell concentration, high cell density and reasonable formula compatibility

Active Publication Date: 2015-09-30
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, seawater f / 2 medium or seawater No. 3 medium is commonly used to cultivate Phaeodactylum tricornutum, but this medium is suitable for the growth of many kinds of single-celled algae, which may easily lead to culture failure.

Method used

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  • Phaeodactylum tricornutum culture medium

Examples

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Effect test

Embodiment 1

[0011] (1) Use natural seawater to configure Phaeodactylum tricornutum culture medium of the following formula: NH 4 NO 3 30mg / L, NaH 2 PO 4 5mg / L, FeCl 3 2mg / L, Na 2 EDTA 3mg / L, Scandium Chloride 0.1mg / L, Triacontanol 0.1mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 ·4H 2 O 23μg / L, MnCl 2 ·4H 2 O 178μg / L, CuSO 4 ·5H 2 O 10μg / L, Na 2 MoO 4 ·2H 2 O 7.3μg / L, CoCl 2 ·6H 2 O 12 μg / L.

[0012] (2) Take Phaeodactylum tricornutum in the logarithmic growth phase and inoculate it into the medium for cultivation. The light is illuminated for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at about 25 degrees Celsius, and the cell concentration of Phaeodactylum tricornutum in the medium was recorded at intervals, as shown in Table 1.

Embodiment 2

[0014] (1) Use natural seawater to configure Phaeodactylum tricornutum culture medium of the following formula: NH 4 NO 3 50mg / L, NaH 2 PO 4 6mg / L, FeCl 3 5mg / L, Na 2 EDTA 6mg / L, Scandium chloride 1mg / L, Triacontanol 1mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 ·4H 2 O 23μg / L, MnCl 2 ·4H 2 O 178μg / L, CuSO 4 ·5H 2 O 10μg / L, Na 2 MoO 4 ·2H 2 O 7.3μg / L, CoCl 2 ·6H 2 O 12 μg / L.

[0015] (2) Take Phaeodactylum tricornutum in the logarithmic growth phase and inoculate it into the medium for cultivation. The light is illuminated for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at about 25 degrees Celsius, and the cell concentration of Phaeodactylum tricornutum in the medium was recorded at intervals, as shown in Table 1.

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Abstract

The invention discloses a phaeodactylum tricornutum culture medium. The phaeodactylum tricornutum culture medium comprises, by mass concentration, 30-50 mg / L of NH4NO3, 5-8 mg / L of NaH2PO4, 2-5 mg / L of FeCl3, 3-6 mg / L of Na2EDTA, 0.005-0.01 mg / L of VB1, 0.01-0.05 mg / L of VB12, 22 to 24 microgram / L of ZnSO4.4H2O, 175-180 microgram / L of MnCl2.4H2O, 9-11 microgram / L of CuSO4.5H2O, 7.0-7.5 microgram / L of Na2MoO4.2H2O and 11-13 microgram / L of CoC12.6H2O. The phaeodactylum tricornutum culture medium further comprises 0.1-1 mg / L of a plant growth regulatory factor, namely, triacontanol, and 0.1-1 mg / L of scandium salt. The culture medium has the advantages of being rapid in growth rate and high in cell density when used for culturing phaeodactylum tricornutum, and is suitable for large-scale batched production.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a Phaeodactylum tricornutum culture medium. Background technique [0002] Phaeodactylum tricornutum belongs to the diatom phylum Pinus algae, and is a primary productivity algae in common marine ecosystems. Phaeodactylum tricornutum is rich in a variety of unsaturated fatty acids, especially eicosapentaenoic acid (EPA), making it an open bait for many precious aquatic animals and a raw material for marine biopharmaceuticals. [0003] At present, seawater f / 2 medium or seawater No. 3 medium is commonly used for culturing Phaeodactylum tricornutum, but this medium is suitable for the growth of a variety of unicellular algae, which can easily lead to culture failure. To solve this problem, we specially designed a medium suitable for the growth of Phaeodactylum tricornutum. SUMMARY OF THE INVENTION [0004] The technical problem to be solved by the present invention is to provide a Phae...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟彭小伟宋佳巍张君纲王琦张云江灵芝孙小雪
Owner NINGBO FUTIAN BIOTECH
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