Method for efficiently collecting phaeodactylum tricornutum

A technology of Phaeodactylum algae and Trigonella, applied in the field of microalgae cultivation, can solve the problems of restricting the development and utilization of fucoxanthin, and achieve the effects of fast flocculation formation, good flocculation and wide pH range

Inactive Publication Date: 2018-07-03
云南爱尔发生物技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Method for efficiently collecting phaeodactylum tricornutum
  • Method for efficiently collecting phaeodactylum tricornutum
  • Method for efficiently collecting phaeodactylum tricornutum

Examples

Experimental program
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Embodiment 1

[0040] After a period of cultivation, 300L of high-density algae solution, OD 680 =5.1, add 1440g of dipotassium hydrogen phosphate and 720g of calcium chloride to the algae solution. After flocculent precipitation occurs, use a centrifugal pump to pump the algae solution into a filter press for filtration. The filter cloth is 1200 mesh. 6.2 kg of Phaeodactylum tricornutum wet algal mud was harvested.

Embodiment 2

[0042] After a period of cultivation, 1000L of high-density algae liquid, OD 680 =5.4. Add 4800g of dipotassium hydrogen phosphate and 2400g of calcium chloride to the algae solution. After flocculent precipitation occurs, the algae solution is pumped into a filter press for filtration with a filter cloth of 1200 mesh. 20.4 kg of Phaeodactylum tricornutum wet algal mud was harvested. Embodiment three:

Embodiment 3

[0043] Embodiment three:

[0044] After a period of cultivation, 2000L of high-density algae liquid, OD 680 =5.6, add 9600g of dipotassium hydrogen phosphate and 4800g of calcium chloride to the algae solution. After flocculent precipitation occurs, the algae solution is pumped into a filter press for filtration with a filter cloth of 1200 mesh. 56.1 kg of Phaeodactylum tricornutum wet algal mud was harvested.

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Abstract

The invention discloses a method for efficiently collecting phaeodactylum tricornutum. The method comprises the following steps: (1) adding calcium chloride and dipotassium phosphate; (2) forming calcium phosphate precipitate; and (3) filtering with a filter press. The method is simple and quick to operate, and does not influence fucoxanthin in phaeodactylum tricornutum cells.

Description

Technical field [0001] The invention belongs to the technical field of microalgae cultivation, and specifically relates to a method for efficiently collecting Phaeodactylum tricornutum. Background technique [0002] Phaeodactylum tricornutum is a typical single-celled microalgae. It has three different forms: ovoid, spindle, and triple radial. These three forms can be transformed under different environmental conditions. For example, under normal liquid culture conditions, there are mostly three radial cells and a small number of spindle cells, neither of which has a siliceous cell wall. The length of the three radial cells is about 10-18 microns (the vertical distance between the two arms), and there is a nucleus in the center of the cell with 1 to 3 pieces of yellow-brown pigment bodies. The spindle cells are 20 microns long and have blunt ends of the arms. At the same time, it is a kind of marine economic single-celled microalgae with very economic value. It belongs to the g...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N1/02C12R1/89
CPCC12N1/02C12N1/12
Inventor 吴澄张吉和杨泽娥郑学军王兴勇张勇
Owner 云南爱尔发生物技术股份有限公司
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