Aureococcus anophagefferens algistat and preparation method for same

A Chrysococcus algae inhibitor, Chryseococcus antifeeding technology, applied in botanical equipment and methods, herbicides, algicides, biocides, etc., can solve the problems of low ecological security, and achieve simple preparation methods , long-lasting effect, fast onset effect of algae inhibition

Inactive Publication Date: 2016-07-20
LIAONING OCEAN & FISHERIES SCI RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to many limitations such as low ecological security, it is dif...

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  • Aureococcus anophagefferens algistat and preparation method for same
  • Aureococcus anophagefferens algistat and preparation method for same
  • Aureococcus anophagefferens algistat and preparation method for same

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Embodiment 1

[0033] The invention relates to an algae-inhibiting agent for Chrysococcus, which is prepared from the following steps: suction-filtering, extracting, concentrating and drying the algae liquid of Phaeodactylum tricornutum.

[0034] A kind of preparation method of described antifeeding Chrysococcus algicide, such as figure 1 shown, including the following steps:

[0035] (1) Phaeodactylum tricornutum is cultivated to the late stage of exponential growth; the expanded cultivation of Phaeodactylum tricornutum: adopt 1000 milliliters of f / 2 culture medium to cultivate, and culture condition is that light intensity is 2000 lux, light-dark cycle D:L=12 hours : 12 hours at 20°C. Shake the algae liquid 3 times a day, cultivate Phaeodactylum tricornutum to the late stage of exponential growth, and the algal cell density is 6.8×10 10 per liter, that is, the total number of algal cells of Phaeodactylum tricornutum is 6.8×10 10 indivual;

[0036] (2) carry out vacuum suction filtratio...

Embodiment 2

[0056] The invention relates to an algae-inhibiting agent for Chrysococcus, which is prepared from the following steps: suction-filtering, extracting, concentrating and drying the algae liquid of Phaeodactylum tricornutum.

[0057] A kind of preparation method of described antifeeding Chrysococcus algicide comprises the steps:

[0058] (1) Phaeodactylum tricornutum is cultivated to the late stage of exponential growth; the expanded cultivation of Phaeodactylum tricornutum: adopt 1000 milliliters of f / 2 culture fluid to cultivate, and culture condition is that light intensity is 2500 lux, light-dark cycle D:L=12 hours : 12 hours at 20°C. Shake the algae liquid 3 times a day, cultivate Phaeodactylum tricornutum to the late stage of exponential growth, and the algal cell density is 6.8×10 10 per liter, that is, the total number of algal cells of Phaeodactylum tricornutum is 6.8×10 10 indivual;

[0059] (2) carry out vacuum suction filtration through the filter membrane of desc...

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Abstract

The invention provides an aureococcus anophagefferens algistat and a preparation method for the same. The preparation method for the aureococcus anophagefferens algistat comprises the following steps: (1) cultivating phaeodactylum tricornutum to a later exponential growth stage to obtain an alga solution; (2) performing vacuum suction filtration on the alga solution to obtain alga cell-free filtrate; (3) performing extraction, reduced pressure concentration and nitrogen concentration on the alga cell-free filtrate to obtain the aureococcus anophagefferens algistat. The aureococcus anophagefferens algistat is derived from natural microalgae, is high in environmental affinity, can quickly act for algal inhibition, and has long-lasting effects; in addition, the preparation method is simple and low in preparation cost, and can be widely applied to inhibition and killing of aureococcus anophagefferens in offshore areas, aquaculture ponds and the like on a large scale.

Description

technical field [0001] The invention belongs to the technical field of water body pollution control, and in particular relates to an antifeeding algae inhibitor for Chrysococcus and a preparation method thereof. Background technique [0002] Aureococcus anophagefferens, belonging to Chrysophyta and Pelagophyceae, is a microalgae with an individual size of 2-3 microns, so its quantity can only be monitored by molecular biological means. From the 1980s to the present, Chrysococcus spp. has repeatedly formed large-scale harmful algal blooms in the United States. During the algal blooms, the cell density is extremely high (109 / liter), and the water body of the algal blooms is yellowish brown, so it is called brown tide. The nomenclature is used by US research and regulatory agencies and accepted by the international harmful algal bloom research community. my country is the third country to experience a brown tide after the United States and South Africa. Since 2009, brown tide...

Claims

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Application Information

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IPC IPC(8): A01N65/03A01P13/00C02F1/50
CPCA01N65/00A01N65/03C02F1/50
Inventor 宋伦毕相东孙明董少杰王昆郭永军邵泽伟张树林吴金浩李楠田金李爱
Owner LIAONING OCEAN & FISHERIES SCI RES INST
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