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Culture medium and culture method for mixing culturing of phaeodactylum tricornutum bohlin and nitzschia closterium

A culture method and mixed culture technology, applied in the field of microalgae culture, can solve the problems of long doubling time of algae cells, slow growth speed, troublesome operation, etc., and achieve the effect of shortening the culture period, slow growth speed and easy operation

Active Publication Date: 2014-06-04
江苏津沂菊源生物健康产业研究院有限公司
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Problems solved by technology

[0006] The cultivation of Phaeodactylum tricornutum and Nitzschia lunata traditionally use f / 2 medium and open cement pond culture, the doubling time of algal cells is long, the growth rate is slow, the investment is large, the yield is low, easy to pollute, and the operation is troublesome

Method used

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  • Culture medium and culture method for mixing culturing of phaeodactylum tricornutum bohlin and nitzschia closterium

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Embodiment Construction

[0020] 1) The Phaeodactylum tricornutum and Nitzschia minor for inoculation are purified in advance (conventional methods in the art, as long as the purification can be realized, such as Soxhlet extraction method), to ensure that the algal species are in the exponential growth phase during inoculation;

[0021] 2) 920 plant growth stimulant is a white crystalline powder, which is not easy to dissolve in water. It can be fully dissolved in alcohol first (generally dissolve with 50 ml of alcohol or 60% white wine for more than 1 hour per gram), and then add water according to the required concentration dilution;

[0022] 3) Due to iron citrate (FeC 6 h 5 o 7 ·5H 2 O) It is difficult to dissolve, you can add a small amount of tap water and heat it slightly to 80-90°C on the stove, and keep stirring until it is completely melted;

[0023] 4) Various nutrient salts should be added in the order provided in the formula to avoid chemical reactions;

[0024] 5) All kinds of nutrie...

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Abstract

The invention discloses a high-yield mixing culturing method of phaeodactylum tricornutum bohlin and nitzschia closterium, aiming at solving the problems that the phaeodactylum tricornutum bohlin and the nitzschia closterium are individually cultured with an f / 2 culture medium in an open cement pit, which causes low growth rate, low yield, easy polluting, and complex operation in the traditional culture method. The culture medium, disclosed by the invention, is characterized in that a mineral water barrel is utilized for mixing culturing of the phaeodactylum tricornutum bohlin and the nitzschia closterium, thus high fatty acid content can be obtained, the cost is low, the pollution is difficult to cause, the operation is easy, and the workload is greatly decreased; in addition, peptone, agar, maltose, potassium sorbate, glucose, sea mud extracting liquor, soil extract liquor, human urine, and cow and sheep manure leaching liquor are added to the culture medium, so that the nutrition is comprehensive and balanced, the growth speed of algae can be greatly raised, and the output is increased by 180%; moreover, the mixing culturing enables the mutualism of the two kinds of algae, and the two kinds of algae can absorb advantages mutually so as to promote each other; nutritive salt is fully utilized, so that the economic benefit is also increased.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, and in particular relates to a high-yield technology for mixed cultivation of Phaeodactylum tricornutum and Nitzschia minor. Background technique [0002] Phaeodactylum tricornutum Bohlin is a marine unicellular microalgae belonging to Diatomphyta, Featheria, Phaeodactylaceae, Phaeodactylum family, Phaeodactylum genus. The cells are ovoid, spindle-shaped or radial, and the shape can change under different environmental conditions. The oval cells are 8 microns long and 3 microns wide, with only one siliceous shell surface, a nucleus in the center, and 1-3 pieces of yellowish-brown pigment bodies. Spindle cells are 20 microns long with blunt arms at the ends. Parallel division into 2 identical cells for reproduction. Cells do not have siliceous shells, so they are different from diatoms during fission, and the algal bodies do not shrink. Live within the salinity range of 9-92‰, and the opt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 王培磊
Owner 江苏津沂菊源生物健康产业研究院有限公司
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