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Method for increasing fucoxanthin content of Phaeodactylum tricornutum by using methyl jasmonic acid

A technology of Phaeodactylum sp. fucoxanthin and methyl jasmonic acid is applied in the field of biomedicine and achieves the effects of increased content, low cost, simple and convenient operation

Inactive Publication Date: 2015-04-22
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the use of various elicitors to increase the content of fucoxanthin

Method used

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  • Method for increasing fucoxanthin content of Phaeodactylum tricornutum by using methyl jasmonic acid
  • Method for increasing fucoxanthin content of Phaeodactylum tricornutum by using methyl jasmonic acid
  • Method for increasing fucoxanthin content of Phaeodactylum tricornutum by using methyl jasmonic acid

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1 methyl jasmonic acid affects the growth of Phaeodactylum tricornutum

[0037] Take 21 groups of 300ml sterilized MAD culture solution, respectively, and insert them into Phaeodactylum tricornutum algae at a ratio of 1:10. Phaeodactylum tricornutum were treated with methyljasmonic acid at concentrations of 50 μmol / L, 100 μmol / L, 200 μmol / L, 500 μmol / L, 1000 μmol / L, and 2000 μmol / L, and 3 parallel groups without any treatment. From the day when Phaeodactylum tricornutum was inserted into the culture medium, the concentration of the algae liquid in each group needs to be measured by a spectrophotometer every day, and then measured for another 6 days.

[0038] Such as figure 1As shown, after treatment with methyl jasmonic acid (MeJA), the growth of Phaeodactylum tricornutum was affected differently, and the absorbance value in the stable phase was Meja(50μmol / L)>Meja(200μmol / L)>Meja( 100 μmol / L)>Meja (500 μmol / L)>Meja (2000 μmol / L)>Meja (1000 μmol / L). When t...

Embodiment 2

[0040] Take 7 groups of 400ml sterilized seawater and 7 groups, add 400 μl of MAD mother solution respectively, add Phaeodactylum tricornutum at a ratio of 1:10, and after cultivating for 2 days, add methyl jasmonic acid to the 6 groups of algae culture solution, and The concentrations are 50μmol / L, 100μmol / L, 200μmol / L, 500μmol / L, 1000μmol / L, 2000μmol / L.

[0041] Such as figure 2 As shown, from the growth curve of Phaeodactylum tricornutum, it can be known that the first day to the sixth day is the logarithmic growth period, the sixth day to the seventh day is the stable period, and the following days are the decay period. Therefore, it can be known that Phaeodactylum tricornutum has reached the end of the logarithmic phase after the 6th day of cultivation, and the maximum biomass can be obtained. Therefore, the algae liquid was collected after 6 days of cultivation.

[0042] Take 80ml of each group of Phaeodactylum tricornutum solution into a centrifuge tube, centrifuge a...

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Abstract

The present invention discloses a method for increasing the fucoxanthin content of Phaeodactylum tricornutum by using methyl jasmonic acid. The method comprises: mixing sterilized seawater and an MAD mother liquor to obtain a nutrition liquid, adding a Phaeodactylum tricornutum mother liquor to the nutrition liquid to culture, culturing for 2 days, adding methyl jasmonic acid, continuously culturing for 6 days, collecting the algae liquid, carrying out centrifugal separation, removing the supernatant, collecting the algae, carrying out a freeze-drying treatment on the algae for 2 days, and grinding for spare. According to the present invention, with the method, the fucoxanthin content in the Phaeodactylum tricornutum can be significantly increased, the operations are simple and convenient, the cost is low, the production cycle is short, and the method is suitable for batch production and industrial production.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for increasing the fucoxanthin content of Phaeodactylum tricornutum with methyl jasmonic acid. Background technique [0002] Fucoxanthin is a class of orange-yellow carotenoids widely present in brown algae and diatoms. Its structure is quite special, with a conjugated double bond, epoxy group, and carbonyl group, which together form a polyene chain compound. Since its structure contains oxygen atoms, it also belongs to lutein and has antioxidant effect. Cardiovascular disease, cancer, and chronic diseases have now become the diseases with the highest mortality rates. The probability of obesity in developed and developing countries is increasing rapidly year by year. However, there are many reasons for these diseases and this phenomenon, and the treatment of drugs is not always effective. Researchers are studying the functional ingredients in food that have therap...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/12C12R1/89
CPCC12N1/38
Inventor 朱帅旗龚一富刘浩马颖瑞张静周丽亚王何瑜
Owner NINGBO UNIV
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