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Phaeodactylum tricornutum culture medium

A technology for Phaeodactylum tricornutum and culture medium, which is applied in the field of Phaeodactylum tricornutum culture medium, can solve the problems of unsuitable Phaeodactylum tricornutum seed solution preparation, low growth rate, etc., and achieves better effect, high biomass, and cell density. big effect

Inactive Publication Date: 2018-06-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantage of this application is that the invention added additional plant growth regulators on the basis of autotrophic culture, which can promote the growth of Phaeodactylum tricornutum to a certain extent, but the growth rate of Phaeodactylum tricornutum under autotrophic conditions is obviously low Suitable for heterotrophy and polyculture, not suitable for the preparation of seed liquid in large-scale cultivation of Phaeodactylum tricornutum

Method used

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  • Phaeodactylum tricornutum culture medium
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  • Phaeodactylum tricornutum culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1: Study on the change trend of different glycerol concentrations on the growth of Phaeodactylum tricornutum and the content of fucoxanthin in the cells

[0054] 1.1 Design of culture conditions for Phaeodactylum tricornutum

[0055] Use sea salt to configure the following basal medium for Phaeodactylum tricornutum with a salinity of 20g / L: NaNO 3 0.85g / L; NaH 2 PO 4 ·H 2 O 10mg / L; Na 2 SiO 3 9H 2 O 30mg / L; FeCl 3 ·6H 2 O 3.15mg / L; Na 2 EDTA·2H 2 O4.36mg / L; CuSO 4 ·5H 2 O 9.8ug / L; Na 2 MoO 4 2H 2 O 6.3ug / L; ZnSO 4 ·7H 2 O 22ug / L; CoCl 2 ·6H 2 O 10ug / L; MnCl 2 4H 2 O 180ug / L.

[0056] Add different concentrations of glycerol (0, 0.05, 0.1, 0.15mol / L) to the basal medium, adjust the pH to 8.0, and sterilize under high temperature and high pressure (121°C, 20min). Inoculate seed solution (initial OD450nm at about 0.5, cell number 1X10 6 ), placed in a constant temperature shaker at 20°C for cultivation, with light intensity of 1000±500LUX an...

Embodiment 2

[0059] Example 2: Study on the change trend of different nitrogen sources on the growth of Phaeodactylum tricornutum and the content of fucoxanthin in the cells

[0060] 2.1 Culture condition design

[0061] Use sea salt to configure the following Phaeodactylum basal medium with a salinity of 20g / L: glycerol 0.1mol / L; NaH 2 PO 4 ·H 2 O 10mg / L; Na 2 SiO 3 9H 2 O 30mg / L; FeCl 3 ·6H 2 O 3.15mg / L; Na 2 EDTA·2H 2 O4.36mg / L; CuSO 4 ·5H 2 O 9.8ug / L; Na 2 MoO 4 2H 2 O 6.3ug / L; ZnSO 4 ·7H 2 O 22ug / L; CoCl 2 ·6H 2 O 10ug / L; MnCl 2 4H 2 O 180ug / L.

[0062] Different kinds of nitrogen sources (sodium nitrate, urea, tryptone) were added to the basal medium respectively, and the concentration of the nitrogen source was 0.01mol / L. Adjust the pH to 8.0. Except for urea, which was sterilized by 0.22um sterile filter membrane, the others were sterilized by high temperature and high pressure (121°C, 20min). Inoculate seed solution (initial OD450nm at about 0.5, cell number...

Embodiment example 3

[0065] Implementation case 3: Study on the change trend of different tryptone concentrations on the growth and intracellular fucoxanthin content of Phaeodactylum tricornutum

[0066] 3.1 Culture conditions and design

[0067] Use sea salt to configure the following Phaeodactylum basal medium with a salinity of 20g / L: glycerol 0.1mol / L; NaH 2 PO 4 ·H 2 O 10mg / L; Na 2 SiO 3 9H 2 O 30mg / L; FeCl 3 ·6H 2 O 3.15mg / L; Na 2 EDTA·2H 2 O4.36mg / L; CuSO 4 ·5H 2 O 9.8ug / L; Na 2 MoO 4 2H 2 O 6.3ug / L; ZnSO 4 ·7H 2 O 22ug / L; CoCl 2 ·6H 2 O 10ug / L; MnCl 2 4H 2 O 180ug / L.

[0068] Add tryptone with different nitrogen content: 0.005, 0.01, 0.015, 0.02mol / L to the basal medium, adjust the pH to 8.0, and sterilize under high temperature and high pressure (121°C, 20min). Inoculate seed solution (initial OD450nm at about 0.5, cell number 1X10 6 ), placed in a constant temperature shaker at 20°C for cultivation, with light intensity of 1000±500LUX and rotation speed of 150rpm. ...

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Abstract

The invention discloses a phaeodactylum tricornutum culture medium. A formula of the culture medium comprises tryptone. Preferably, the formula contains a mixed nitrogen source. The culture medium isfavorable for accelerating the growth speed of the phaeodactylum tricornutum, increasing the cell density and promoting the fucoxanthin accumulation so as to improve the culture efficiency. The phaeodactylum tricornutum culture medium is particularly suitable for large-scale culture.

Description

technical field [0001] The invention relates to a medium for microalgae, in particular to a medium for Phaeodactylum tricornutum. Background technique [0002] Fucoxanthin is a carotenoid involved in photosynthesis, accounting for more than 10% of the total carotenoids in nature, and widely exists in algae such as brown algae, diatoms and golden algae. Studies by Peng J and others have found that fucoxanthin has significant effects in anti-cancer, anti-endotoxin-inflammation, anti-oxidation, anti-malarial, and protection of liver and blood vessels. In addition, studies by Kuipers R S and others have confirmed that fucoxanthin can significantly improve insulin resistance properties, reduce blood glucose levels, and have a significant effect on weight loss. [0003] At present, the raw materials used to extract fucoxanthin are mainly large seaweeds such as kelp and wakame. However, due to the low content of fucoxanthin in macroalgae, the high cost of cultivation, the difficu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 魏东宋培钦杨润青
Owner SOUTH CHINA UNIV OF TECH
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