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Method for preparing biodiesel from marine microalgae

A biodiesel, marine microalgae technology, applied in the preparation of biofuels, liquid hydrocarbon mixtures, petroleum industry, etc., can solve problems such as long-term growth strategy, long growth cycle, climate impact, etc. , to achieve the effect of good quality, simple cost and high output

Inactive Publication Date: 2016-08-17
卞佳林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the disadvantages of occupying arable land area, long growth cycle, and being affected by climate, oil plants cannot become a long-term strategy for the supply of raw material oil for biodiesel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for preparing biodiesel based on marine microalgae, comprising the following steps:

[0021] Step 1, washing Phaeodactylum tricornutum, removing water through plate and frame pressure filtration, to obtain wet algae;

[0022] Step 2: In parts by weight, take 1 part of the wet algae obtained in Step 1 and add it to 5 parts of water, then add 0.01 part of cellulase, 0.03 part of protease, and 0.04 part of sodium citrate, mix, enzymatically hydrolyze at 45°C for 5 hours, and sterilize Enzyme, obtain enzymolysis solution;

[0023] Step 3, adjust the pH of the enzymolysis solution obtained in step 2 to 9.0, add an equal volume of chloroform-methanol, extract, and take the organic phase;

[0024] Step 4, adding the organic phase obtained in Step 2 to a mixed solution of concentrated sulfuric acid, tetrahydrofuran and methanol of 2 times the volume, shaking the shaker for 4 hours and then standing still to obtain the upper ester phase;

[0025] Step 5: wash the este...

Embodiment 2

[0029] A method for preparing biodiesel based on marine microalgae, comprising the following steps:

[0030] Step 1, washing Phaeodactylum tricornutum, removing water through plate and frame pressure filtration, to obtain wet algae;

[0031] Step 2: In parts by weight, take 1 part of the wet algae obtained in Step 1 and add it to 8 parts of water, then add 0.03 part of cellulase, 0.06 part of protease, and 0.07 part of sodium citrate, mix, enzymatically hydrolyze at 50°C for 5 hours, and sterilize Enzyme, obtain enzymolysis solution;

[0032] Step 3, adjust the pH of the enzymolysis solution obtained in step 2 to 9.5, add an equal volume of chloroform-methanol, extract, and take the organic phase;

[0033] Step 4, adding 3 times the volume of the mixed solution of concentrated sulfuric acid, tetrahydrofuran and methanol to the organic phase obtained in step 2, shaking the shaker for 4 hours and then standing still to obtain the upper ester phase;

[0034] Step 5: wash the es...

Embodiment 3

[0038] A method for preparing biodiesel based on marine microalgae, comprising the following steps:

[0039] Step 1, washing Phaeodactylum tricornutum, removing water through plate and frame pressure filtration, to obtain wet algae;

[0040] Step 2: In parts by weight, take 1 part of the wet algae obtained in Step 1 and add it to 6 parts of water, then add 0.04 part of cellulase, 0.05 part of protease, and 0.07 part of sodium citrate, mix, enzymolyze at 45°C for 2 hours, and extinguish Enzyme, obtain enzymolysis solution;

[0041] Step 3, adjust the pH of the enzymolysis solution obtained in step 2 to 10.0, add an equal volume of chloroform-methanol, extract, and take the organic phase;

[0042] Step 4, adding 3 times the volume of the mixed solution of concentrated sulfuric acid, tetrahydrofuran and methanol to the organic phase obtained in step 2, shaking the shaker for 4 hours and then standing still to obtain the upper ester phase;

[0043] Step 5: wash the ester phase o...

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Abstract

The invention provides a method for preparing biodiesel from marine microalgae, comprising the steps of: cleaning Phaeodactylum tricornutum, carrying out plate-and-frame filter pressing to remove water, adding the wet Phaeodactylum tricornutum into water, adding cellulase, protease and sodium citrate, mixing the materials, carrying out enzymolysis at 45-55 DEG C for 2-5h, carrying out enzyme deactivation, regulating the pH value of the obtained enzymolysis liquid to 9.0-10.0, adding an equal volume of chloroform-methanol, carrying out extraction, adding the organic phase to mixed liquid of concentrated sulfuric acid, tetrahydrofuran and methanol, standing after shaker oscillation, washing and centrifuging the obtained ester phase with petroleum ether-95v / v%ethanol to obtain an oil phase and concentrating the oil phase. The preparation method has the advantages of high yield of biodiesel, good quality, simple production process and low cost and can be widely popularized.

Description

technical field [0001] The invention belongs to the technical field of bioenergy, and in particular relates to a method for preparing biodiesel with marine microalgae as a base material. Background technique [0002] Biodiesel refers to methyl ester or ethyl ester fuel made from oil crops, wild oil crops, engineering microalgae and other aquatic vegetable oils, as well as animal oils, waste cooking oil, etc., through transesterification process. As a clean biofuel that can replace petrochemical diesel, the performance of biodiesel is basically the same as that of current petrochemical diesel, and it has good environmental characteristics and biodegradability, so it has broad development prospects. [0003] The choice of oil raw materials is mainly determined by the cost of raw materials and the extensiveness of their sources. According to statistics, the cost of biodiesel preparation is the cost of raw materials, so using cheap raw materials and increasing the conversion ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C10G1/04C10G1/00
CPCC10G1/00C10G1/04C10G2300/1014Y02E50/10
Inventor 不公告发明人
Owner 卞佳林
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