62results about How to "Rapid Detection Analysis" patented technology

The invention discloses a core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material, and a preparation method and application thereof. According to the molecularly imprinted solid phase extraction magnetic material, surface-aminated Fe3O4 nano-particles serve as a magnetic core, and a rhodamine 6G molecularly imprinted polymer membrane serves as a shell. The core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material has a memory function for a three-dimensional structure of rhodamine 6G molecules, and rhodamine 6G can be specifically and selectively separated and enriched. The core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material is applied to selective adsorption and dynamic extraction and separation of a trace amount of rhodamine 6G in the food sample analysis pretreatment; the rhodamine 6G can be quickly separated and detected; according to the method, the recovery rate is high and the relative standard deviation is small; the core-shell rhodamine 6G molecularly imprinted solid phase extraction magnetic material has the characteristics of small using amount of organic solvents, simplicity, quickness and high efficiency in operation, and the like.

The invention discloses a wide-range electron beam excited fluorescence imaging and spectral measurement device and a method thereof. The wide-range electron beam excited fluorescence imaging and spectral measurement device comprises a electron microscope scanning system, a scanning signal generator, a wide-range fluorescence collecting, coupling and transmitting system, a fluorescence intensity detector, a fluorescence spectral detector, a synchronous scanning signal collector and a cooperative control and data processing output system. Since a wide-range fluorescence coupler is introduced, fluorescence signals excited within an imaging view field by the microscope scanning system can be converged and coupled to a fluorescence transmission light path with high collection efficiency, the problems that, to images acquired by wide-range fluorescence scanning imaging, it is difficult to calculate and compare fluorescence excitation intensity or fluorescence yield and fluorescence spectruminformation of different positions according to uniform standards are solved, and wide-range rapid detection and analysis on the basis of electron beam excited fluorescence signals can be completed.

The invention belongs to the technical field of the gas detection, and relates to a detection device and method of non-methane total hydrocarbon. The detection device comprises a multi-channel sampling valve, a first quantitative loop, a second quantitative loop and a detector; switchable quantitative mode and detection mode are formed through the multi-channel sampling valve; the quantitative mode is a sample gas channel including the first quantitative ring and the second quantitative ring; the detection mode is two carrier gas channels; one carrier gas channel comprises the first quantitative ring and a methane column, and the other carrier gas channel comprises the second quantitative ring and a total hydrocarbon column; and two carrier gas channels are connected with a detector. Through the detection device and method of non-methane total hydrocarbon provided by the invention, the detection of the non-methane total hydrocarbon can be realized since a fourteen-way valve is adoptedto conform to the dual column in the HJ38-2017 standard, the flow is simplified, the problem that the existing non-methane total hydrocarbon detection device is complex in structure can be solved at the same time, and the detection device has the advantages of being simple in structure, and convenient for installation and operation.

The invention discloses an electron beam excitation fluorescence large-range direct detection imaging apparatus and a method thereof. The imaging apparatus comprises a scanning electron microscope system, a scanning signal generator, a fluorescence collection coupling system, a semiconductor photodetector, a scanning synchronization signal collector and a synergic control and data processing output system. According to the present invention, by using the modular architecture, the configuration adjustment and the subsequent upgrade of each module are flexible and convenient; and by introducingthe large-area semiconductor photoelectric detection chip of the semiconductor photodetector, the fluorescence excited by the scanning electron microscope system in the large imaging visual field range can be concentrated and coupled to the semiconductor photodetector at the same high collection efficiency, such that the problem that the fluorescence excitation intensities or the fluorescence excitation yields at different positions of the image obtained through the large-range fluorescence scanning imaging are difficultly calculated and compared by using the standard is solved so as to complete the large-range rapid detection and analysis based on the electron beam excitation fluorescence signals.

The invention discloses a preparation method and detection method of a fluorescence sensor for an aflatoxin toxin production gene nor-1. The fluorescence sensor is a long-arm hairpin probe based on specific recognition of the aflatoxin toxin production gene nor-1; and the two ends of the long-arm hairpin probe are provided with NH2 and SH correspondingly, a CdTe/CdS quantum dot of an upper core-shell structure is modified by the NH2, and upper gold nanoparticles (AuNPs) are modified by the SH. The preparation method comprises the steps: a CdTe/CdS quantum dot solution is prepared for standby application; the AuNPs are prepared for standby application; and the long-arm hairpin probe is modified. Detection comprises construction of the fluorescence sensor, when the target nor-1 appears, thenor-1 and the hairpin probe are subjected to specific complementary pairing, the hairpin probe is straightened, the distance between the CdTe/CdS quantum dot and the AuNPs is enlarged, transferring offluorescent resonance energy is blocked, thus fluorescence of the CdTe/CdS quantum dot is recovered, the concentration of the nor-1 is tested through the fluorescence recovery intensity, and thus thenor-1 is quickly detected. The prepared fluorescence sensor has the advantages that flexibility, simpleness, quickness and the like are achieved, the toxin production gene is detected from the source, the cost is low, and detection is quick.

The invention provides a method for constructing a fluorescence resonance energy transfer (FRET) sensor based on graphene and quantum dots and a method for detecting a CaMV35S promoter by means of the sensor. The method comprises the steps of 1, preparing multiwalled carbon nanotubes/graphene oxide nanoribbons (MWCNTs/GONRs) dispersion liquid; 2, preparing nitrogen sulfur heterotactic graphene quantum dots (NSGQDs) solution; 3, preparing nitrogen sulfur heterotactic graphene quantum dots-capture probe (NSGQDs-ssDNA) dispersion liquid; 4, constructing the FRET sensor; 5, constructing a standard curve; 6, detecting transgenic soybean by means of the FRET sensor constructed based on MWCNTs/GONRs-NSGQDs-ssDNA. The FRET sensor has the advantages of being convenient and flexible to operate and high in analysis speed and is suitable for quick detection and analysis.

The invention discloses a concealed channel detection method based on a multi-scale flow analysis technology, and the method comprises the following steps: obtaining the information of a detection andanalysis scene, and collecting the network traffic data in the detection and analysis scene through DPDK; preprocessing the collected network traffic data to obtain a single data packet, a session flow and a communication flow; establishing a corresponding meta-database according to the related characteristics of the single data packet, the session flow and the communication flow; importing the obtained single data packet, session flow and communication flow into the corresponding meta-database for black and white matching to obtain priori knowledge; establishing a multi-scale feature analysis model; and importing the obtained single data packet, session flow and communication flow into the multi-scale feature analysis model for analysis and detection, and generating a detection report. According to the invention, the false alarm rate and missing report rate of concealed channel detection can be effectively reduced, the comprehensiveness and accuracy of concealed channel detection areimproved, and the transmission security of network information is ensured.

The invention discloses a low-molecular-weight substance mass spectrometry detection method based on a palladium-gold core-shell micro-nano material, and particularly relates to application of the low-molecular-weight substance mass spectrometry detection method in serum low-molecular-weight substance detection. The palladium-gold alloy core-shell micro-nano particles are used as a matrix material, a serum sample does not need to be subjected to any pretreatment steps such as enrichment or separation, and low-molecular-weight substances in serum can be efficiently and quickly detected and analyzed by taking a very small amount of sample. The detection method is high in accuracy, low in cost and high in detection flux, meets the requirements of clinical serum detection, and has clinical application potential.

The invention discloses a method for rapidly detecting and analyzing the quality of sericite. The method comprises the following steps: acquiring a physical structure of a sericite sample to be detected by adopting an X-ray diffraction analyzer; acquiring the element content of the sericite sample to be detected by adopting a microcomputer multi-element analyzer, and acquiring spectral information of the sericite sample to be detected by adopting a spectrum analyzer; analyzing the physical structure, element content and spectral information of the sericite sample to be detected by adopting an optimal correction model, so as to obtain a quality analysis result of the sericite sample to be detected, wherein a process of establishing the optimal correction model comprises the following step: S1: acquiring the physical structures of the plurality of sericite samples by the X-ray diffraction analyzer under the conditions that the working voltage is 38kV-42kV, the working current is 28mA-32mA and the wavelength is 10-20 angstroms, so as to obtain information of the plurality of physical structures. The method disclosed by the invention can be used for comprehensively and rapidly detecting and analyzing the quality of the sericite, and is low in cost and high in detection and analysis efficiency.

The invention discloses a primer and a probe for the real-time fluorescent PCR detection of transgenic alfalfa J163 strains and a detection method and applications thereof. For transgenic alfalfa J163 strains which are provided by Monsanto Company in America and do not have the Security Certificate of Agricultural Genetically Modified Organisms awarded by the Ministry of Agriculture in China, the invention provides a pair of specific primer and probe for the first time, through the optimization of real-time fluorescent PCR detection systems, a specific real-time fluorescent PCR detection system for alfalfa J163 strains is successfully established, and the system is simple in operation, good in specificity and repeatability, accurate in detection results and high in stability, detects that the lowest DNA concentrations is 15 pg, and can quickly, accurately and steadily carry out detection analysis and authentication on the components of transgenic alfalfa J163 strains.

The invention discloses a high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method for the determination of indaziflam and metabolites of the indaziflam. The method comprises the steps as follows: establishing a standard working curve of a soil matrix of a known concentration gradient of indaziflam and four metabolites of the indaziflam by the HPLC-MS/MS; determining theresidual amount of the indaziflam and the four metabolites of the indaziflam in a soil sample extraction and purification liquid by the HPLC-MS/MS, recording peak areas of extracted ion chromatograms, quantifying the matrix with an external standard method, obtaining measured values of the indaziflam and the four metabolites of the indaziflam in the soil sample extraction and purification liquid; and then substituting the measured values into a quantitative calculation formula to finally obtain the residual amount of the indaziflam to be detected and the four metabolites of the indaziflam insoil samples. The method provided by the invention has the advantages that the sample pretreatment operation is simple and convenient, the dosage of an organic solvent is small, the target pesticideextraction rate is high, the purification effect is good, and the instrument analysis time is short. In addition, the instrument detection limit and the method detection limit are very low.

The invention discloses a boiler water-cooled wall temperature distribution rapid detection, analysis and evaluation system. The system comprises the following steps: cleaning the surface of a water-cooled wall pipe; positioning and marking: numbering the wall pipes in the horizontal counterclockwise direction and marking the numbered wall pipes; collecting and recording wall temperature data of the water-cooled wall: moving a thermal infrared imager to shoot the water-cooled wall, wherein an obtained heat map comprises two marks; water wall pipe position identification: automatically identifying and judging the arrangement and position distribution of the wall pipes through marks on the heat map; analyzing the wall temperature: automatically extracting the highest temperature, the lowesttemperature and the average temperature of each water wall pipe by a computer; alarming when the wall temperature of the water-cooled wall is abnormal: the system gives an alarm when the wall temperature is abnormal; report generation and processing: selecting data and information needing to be formed into a report from a database, and automatically generating the report; and alarm query statistics: querying alarm record data according to different categories. The method is simple and accurate in wall pipe number positioning and identification, can quickly identify, extract and sort out the heat map, and is low in cost and high in accuracy.

The invention relates to a sample pretreatment and amplification integrated nucleic acid analysis system based on a pipette tip and the application of the system. According to the system, a nucleic acid extraction card integrated on the pipette tip is mainly used for pretreating the sample, and the nucleic acid is extracted by use of a solid-phase extraction method; next, a hand-held heating table is utilized to perform nucleic acid amplification on a nucleic acid reaction liquid; in addition, an ultraviolet lamp is used for visually judging and reading the result of detection by use of naked eyes; as a result, the real-time detection and the field detection of the nucleic acid sample are realized. The nucleic acid analysis system can be widely applied to nucleic acid detection and analysis in the fields of environmental detection, disease diagnosis, inspection andquarantine and medicolegal expertise.

The invention provides a high-flux drug detection micro-fluidic chip. The high-flux drug detection micro-fluidic chip consists of a substrate layer, a modification layer and a reaction layer, wherein the reaction layer consists of n reaction modules; each reaction module consists of an entrance, a reaction channel and an outlet. The chip modified by polylysine is hydrophilic, facilitates a sample to be detected to flow into the chip, and has an adsorption effect on drug complete antigens; the polylysine of the modification layer is matched with PDMS (Polydimethylsiloxane) of the reaction layer, so that the hydrophilic feature of the polylysine can be combined with the high-protein adsorption feature of the PDMS to achieve a better detection effect; detection of different samples occurs in different reaction channels, so that the phenomenon of mutual interference is avoided, and non-specific adsorption is lowered; a single chip is provided with a plurality of reaction modules to realize high-flux rapid result detection and analysis; the sizes, intervals and number of the reaction modules can be adjusted as required to realize simultaneous field detection of a plurality of samples and drugs, so that different detection requirements are met.

The invention discloses a detection analysis method of sodium thiocyanate. The method comprises the following steps: (1) preparing a sodium thiocyanate standard solution; (2) dividing milk samples into a detection sample and a sample to be labeled, adding the sodium thiocyanate standard solution into the sample to be labeled to obtain a labeled sample; (3) respectively adding a sodium thiocyanate detection reagent A into the detection sample and the labeled sample, shaking for uniformly mixing, collecting supernatant, adding a sodium thiocyanate reagent B, and uniformly shaking; and (4) comparing the detection sample with the labeled sample, observing and analyzing the color of the detection sample relative to the labeled sample, and determining the content of the sodium thiocyanate in the detection sample by virtue of a labeling amount of the labeled sample. The monitoring analysis method integrates the high speed of an ordinary kit detection method and high precision of a chromatographic analysis method, and has good effect for improving the detection and analysis efficiency and detection and analysis precision.

The invention discloses an accurate and rapid organophosphorus pesticide detection method for vegetables. The method comprises the following steps: preparing a standard sample solution; evenly mixingacetone and cyclohexane to obtain eluent; putting vegetables and silicon dioxide into a grinding machine to be ground and smashed with the grinding pressure being 0.12-1. 0.18 MPa and with the grinding time being 20-20 min. 40 minutes to obtain a sample; sequentially adding anhydrous sodium sulfate, a sample and anhydrous sodium sulfate to the bottom of the injector, compacting, leaching with theeluent, flowing out through a needle hole part of the injector, collecting the eluent, volatilizing the eluent in a ventilation cabinet until the eluent is dry, adding acetone for dissolving, and fixing the volume to obtain a to-be-detected solution; and analyzing by using a gas chromatography detector. The method disclosed by the invention is simple, high in detection sensitivity, high in reproducibility and high in detection speed.

The invention discloses a preparation method and micromolecule detection application of a metal organic framework material, and relates to the field of metal organic framework materials. The preparation method comprises the steps that: dimethylformamide, ethyl alcohol, deionized water, triethylamine and terephthalic acid are mixed and added into a closed container, and metal chloride is added, so that a mixture can be formed; centrifuging and washing are performed after water bath ultrasonic treatment; then dispersing is performed in deionized water, and low-speed centrifugal separation is performed to obtain the ultrathin metal organic framework material; and the ultrathin metal organic framework material is applied to small molecule detection. According to the framework material and the method, the metal ions and the thickness in the metal organic framework material can be accurately adjusted, the defects of a traditional matrix are overcome, the sample size is low, and serum small molecules can be rapidly detected with high throughput and high sensitivity.